1963
DOI: 10.1038/198488a0
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Relaxing Factor in Extracts of Blood Platelets and its Function in the Cells

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Cited by 35 publications
(7 citation statements)
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“…The following day the platelets were centrifuged in a graduated conical centrifuge tube in a GLC centrifuge at 1400 g for 30 min. The pellets were resuspended in 3 vol of homogenizing medium (30 mM KCI, 5 mM MgC12, 10 mM potassium oxalate, and 20 mM Tris-HCI, pH 7.0) (Grette, 1963). 1 ml portions were homogenized in a smooth-walled glass tube with a motor-driven teflon pestle (Arthur H. Thomas Co., Philadelphia, Pa., Cat.…”
Section: Preparation Of Platelet Fractionsmentioning
confidence: 99%
“…The following day the platelets were centrifuged in a graduated conical centrifuge tube in a GLC centrifuge at 1400 g for 30 min. The pellets were resuspended in 3 vol of homogenizing medium (30 mM KCI, 5 mM MgC12, 10 mM potassium oxalate, and 20 mM Tris-HCI, pH 7.0) (Grette, 1963). 1 ml portions were homogenized in a smooth-walled glass tube with a motor-driven teflon pestle (Arthur H. Thomas Co., Philadelphia, Pa., Cat.…”
Section: Preparation Of Platelet Fractionsmentioning
confidence: 99%
“…DISCUSSION Although contractile properties have been attributed to many vertebrate cells, apart from muscle, only platelets have so far been shown to contain an actomyosin-like protein. Moreover, it has been claimed on the basis of physicochemical studies that both actin and myosin can be distinguished in platelets (13) and that even a relaxing principle may play a role (30). With improvement in fixation, embedding, and other electron microscopic techniques thin fibrils resembling those in smooth FIGURE 2 Details of glycerinated platelets which have been reacted with HMM.…”
Section: Methodsmentioning
confidence: 99%
“…These changes include pseudopod formation, movement of granules, increased metabolism, and the specific release of certain platelet constituents such as adenine nucleotides, calcium, 5-hydroxytryptamine, and certain enzymes (Holmsen et at., 1969). Release of calcium (Murer, 1969) is of particular interest since comparisons with other secretory cells (Douglas, 1968; Stormorken, 1969) and limited experimental evidence (Grette, 1962;Sneddon, 1972) suggest that Ca2+ may be the agent that mediates other changes.The most potent physiological stimulator of platelets is thrombin, the enzyme that catalyzes conversion of fibrinogen to fibrin as well as other reactions in the overall process of blood coagulation. Thrombin is one of a class of proteolytic enzymes that are distinguishable by an active serine believed to function as a nucleophilic catalyst in the hydrolysis of peptides (for review, see Magnussion, 1971).…”
mentioning
confidence: 99%