Relative role of bone and kidney in the hypercalcaemia associated with the rat walker carcinosarcoma 256

Hurtado, Jesús; Esbrit, Pedro; Rapado, A

doi:10.1016/0277-5379(91)90066-m

Cited by 9 publications

(3 citation statements)

References 13 publications

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“…It is one of the few types of tumor available for experimental studies, with the advantage of being easily obtained in vivo and presenting quick development, but with the disadvantage of presenting great aggressiveness, very quick development and short latency period 58,[83][84][85][86][87][88][89][90][91][92][93] .…”

Section: Carcinosarcomamentioning

confidence: 99%

Influence of pneumoperitoneum on tumor implantation in parietal incision: a comparative study of trocar incision and laparotomy in rats

Kagohara¹,

Costa²

2012

J. Coloproctol. (Rio J.)

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Kagohara OH, Costa SL. Influence of pneumoperitoneum on tumor implantation in parietal incision: a comparative study of trocar incision and laparotomy in rats. J Coloproctol, 2012;32(3): 214-222. ABSTRACT:Videolaparoscopic surgery has enabled great progress in surgical procedures. However, the literature shows great controversy concerning its indication in oncological surgery for possibly increasing the implantation of metastases. This experimental study was carried out to help solve this controversy and analyze the influence of pneumoperitoneum as a cause of this implantation. A comparative study between incisions of trocars of videolaparoscopy and conventional laparotomy was conducted. For this analysis, 30 Wistar rats were divided into two equal groups: laparotomic group (A), that underwent a 5 cm laparotomy, which was left exposed for 15 minutes; and another (B), in which a 3 mmHg pneumoperitoneum was created with CO 2 and two more trocars were inserted, maintaining the insufflation for the same period. In both groups, the tumor, Walker 256 carcinosarcoma, was inoculated in the surgical procedure. The evaluation was performed within the fifth and the seventh postoperative days. The result of the macroscopic evaluation indicated that the tumor invasion in group A reached 93.33% and the implantation in the laparoscopy incision reached 73.33%. Nevertheless, the histopathological exam showed tumor implantation in all incisions of both groups (100%). The study concluded that parietal tumor implantation is not influenced by pneumoperitoneum in incisions of trocars when compared with conventional laparotomy in rats.Keywords: laparoscopy; laparotomy; neoplasm metastasis; implantation; carcinosarcoma. RESUMO:A cirurgia videolaparoscópica proporcionou grande avanço nas técnicas cirúrgicas. No entanto, há controvérsia na literatura sobre sua indicação na cirurgia oncológica por possivelmente aumentar a implantação tumoral de metástases. No intuito de contribuir para esclarecer essa controvérsia, realizou-se este estudo experimental visando analisar a influência do pneumoperitônio como causa dessa implantação. Realizou-se um estudo comparativo entre incisões dos trocartes da videolaparoscopia e da laparotomia convencional. Para isso, foram utilizados 30 ratos Wistar divididos em dois grupos iguais: um laparotômico (A), que foi submetido à laparotomia com 5 cm de extensão, a qual ficou exposta por 15 minutos; e outro (B), no qual se efetuou pneumoperitônio de 3 mmHg com CO 2 e colocaram-se mais dois trocartes, mantendo-se insuflado pelo mesmo período. Em ambos os grupos, inoculou-se o tumor no ato operatório, o carcinossarcoma 256 de Walker. A avaliação deu-se entre o quinto e sétimo dia de pós-operatório. Como resultado na avaliação macroscópica, há invasão tumoral no grupo A de 93,33% e implantação na incisão laparoscópica de 73,33%. No exame histopatológico, porém, revelou-se implantação tumoral em todas as incisões de ambos os grupos (100%). Conclui-se que a implantação tumoral não sofre influência do pneumoperitônio...

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Section: Carcinosarcomamentioning

confidence: 99%

Influence of pneumoperitoneum on tumor implantation in parietal incision: a comparative study of trocar incision and laparotomy in rats

Kagohara¹,

Costa²

2012

J. Coloproctol. (Rio J.)

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“…It reproduces, in part, human humoral hypercalcemia of malignancy (HHM) syndrome and, thus, it can be used as an experimental model of HHM [3]. The role of the kidney in the hyper calcemia associated to Walker-256-bearing rats has been studied and there is evidence for an altered tubular hand ling of calcium (an increase in tubular calcium reabsorp tion) [4], Serum 1,25-dihydroxyvitamin D (l,25(OH),D) levels are often depressed in the human HHM syndrome, however a small group of patients with solid tumors and hypercalcemia have increased 1,25(OH)2 levels [5,6], Con trary to the human HHM syndrome, serum levels of l,25(OH)2D are elevated in Walker 256 tumor-bearing rats [7,8]. The effect of this elevation in intestinal calcium absorption has not been studied.…”

Section: Introductionmentioning

confidence: 99%

Osseous and Intestinal Compartments in the Humoral Hypercalcemia of Malignancy Associated to Walker 256 Tumor in Rats

Torres¹,

Piedra²,

Rapado³

1993

Oncology

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The objective of the present work has been to study some aspects of bone and intestinal compartments in rats with Walker 256 carcinosarcoma, an experimental model of humoral hypercalcemia of malignancy (HHM). The results have been compared to those obtained in control animals and, also, to those obtained in Yoshida sarcoma-bearing rats, which were used as tumoral controls without hypercalcemia. Urinary hydroxyproline/creatinine ratio (OHProl/creat) is increased, in both Walker 256 and Yoshida tumor-bearing animals, showing the nonspecifity of this bone marker. However, serum tar-trate-resistant acid phosphatase (TRAP) levels are increased in Walker 256 tumor-bearing animals, but they are normal in Yoshida tumor-bearing animals, indicating that TRAP is a better index of bone resorption than OHProl/ creat in the HHM syndrome. The decrease of bone calcium content in Walker 256 tumor-bearing rats, not shown by Yoshida-bearing rats, also reflects an increased bone resorption due to HHM. Serum and bone osteocalcin levels are similar in control, Walker 256 and Yoshida tumor-bearing rats, but we observed a decrease in serum alkaline phosphatase levels in Walker 256 and Yoshida tumor-bearing animals, which could also be a nonspecific tumor effect, due to the presence of the neoplasia. Our results support the convenience of the employment of a nonhypercalcemic tumor group as control in the HHM study, in addition to the healthy controls. We have also observed higher 1,25-dihydroxyvitamin D serum levels in Walker 256 tumor-bearing rats than in control and Yoshida tumor-bearing rats. On the other hand, we have found normal levels in the fractional rate of intestinal calcium absorption in Walker-256 tumor-bearing rats, in spite of their high calcium levels, and a significant decrease of this parameter in Yoshida sarcoma-bearing animals. These results support the concomitant contribution of intestinal compartment to hypercalcemia, in the experimental model of HHM studied.

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“…O carcinossarcoma 256 de Walker foi descoberto em 1928 no laboratório de George Walker do John Hopkins University School of Medicine a partir do desenvolvimento espontâneo de um adenocarcinoma de mama em rata, que por sua vez é mantido em vários laboratórios, por sucessivas inoculações (BRITO et al, 2009;DORNELAS et al, 2006). É um dos poucos modelos de tumores, disponíveis para estudos experimentais nas áreas médicas e biológicas em diferentes linhas de pesquisa (BEVILACQUA et al, 1984;BLACK et al, 1994;EARLE, 1935;GUAITANI et al, 1982;HURTADO et al, 1991;KLULBES et al, 1987;MOTA;REZKALLAH-IWASSO, 1981;MUND et al, 2007;GUASTALE, 1992).…”

Section: Tumor De Walker 256unclassified

Avaliação dos efeitos do azul de metileno fotoativado no modelo experimental do Tumor de Walker 256.

Petrellis¹

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Aos meus pais e familiares pelo estímulo, confiança e apoio recebido ao longo da minha vida.Ao Prof. Dr. Rodrigo Álvaro Brandão Lopes Martins, pela oportunidade, confiança e por me aceitar como aluna de doutorado. Aos professoresDr.Durvanei Augusto Maria do Instituto Butantan e Dra. Marília Cerqueira Leite Seelaender do Departamento de Biologia Celular e do Desenvolvimento do ICB, pelo apoio científico e experiência em pesquisas com câncer durante a realização deste trabalho. A Simone Aparecida Teixeira e Rosangela Aparecida dos Santos Eichler pelo apoio e auxílio técnico em análises de biologia molecular recebido durante a realização dos experimentos. A toda e equipe do laboratório, em especial Patrícia de Almeida, pela amizade, carinho, apoio, compreensão, paciência, incentivo e convivência durante este período. Aos amigos Rodney Capp Pallotta e Rodrigo Labat, pela amizade, paciência e ajuda científica recebida durante a realização deste trabalho. Agradeço aos demais professores do Departamento de Farmacologia, em especial ao Prof. Dr. Marcelo Nícolas Muscará e Profa. Dra. Maria Helena Catelli de Carvalho, pela oportunidade e confiança na realização dos experimentos em seus laboratórios. A todos os funcionários do Departamento de Farmacologia do ICB, em especial a Mônica Nunes e Camila Gonçalves Trindade, pela ajuda e orientação nos prazos e datas durante o doutorado. Agradeço a Capes pela conceção da bolsa de doutorado no Departamento de Farmacologia do ICB -USP. "A mente que se abre a uma nova idéia jamais voltará ao seu tamanho original". Albert Einstein Palavras-chave: Terapia Fotodiâmica. Laser. Agentes Fotosensibilizantes. Azul de Metileno. Câncer.Tumor de Walker 256.

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Relative role of bone and kidney in the hypercalcaemia associated with the rat walker carcinosarcoma 256

Cited by 9 publications

References 13 publications

Influence of pneumoperitoneum on tumor implantation in parietal incision: a comparative study of trocar incision and laparotomy in rats

Influence of pneumoperitoneum on tumor implantation in parietal incision: a comparative study of trocar incision and laparotomy in rats

Osseous and Intestinal Compartments in the Humoral Hypercalcemia of Malignancy Associated to Walker 256 Tumor in Rats

Avaliação dos efeitos do azul de metileno fotoativado no modelo experimental do Tumor de Walker 256.

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