Relative Resistances of Microorganisms to Cathode Rays

Pepper, Rollin E.; Buffa, Nancy T.; Chandler, Velma L.

doi:10.1128/aem.4.3.149-152.1956

Cited by 32 publications

(12 citation statements)

References 4 publications

(4 reference statements)

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“…More specifically, Pepper, Buffa, and Chandler (1956) reported no difference in the resistance between aerobic spores as a group and anaerobic spores as a group when subjected to cathode rays. Essentially the same results were obtained in the present study where the destruction rates for the aerobic and anaerobic spores from a heterogeneous flora paralled one another and approached a logarithmic order.…”

Section: Discussionmentioning

confidence: 97%

Influence of Gamma Radiation on the Microflora of Cucumber Fruit and Blossoms123

Etchells¹,

Costilow²,

Bell³

et al. 1961

Applied Microbiology

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Section: Discussionmentioning

confidence: 97%

Influence of Gamma Radiation on the Microflora of Cucumber Fruit and Blossoms123

Etchells¹,

Costilow²,

Bell³

et al. 1961

Applied Microbiology

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“…Some investigators have reported a reduction in radiation resistance of spores in the frozen state (Edwards, Peterson, and Cummings, 1954;Pepper, Buffa, and Chandler, 1956), whereas others have recorded an increase in resistance at low temperatures (Denny et al, 1959;Ingram et al, 1959). Kempe and Graikoski (unpublished data) found that the radiation resistance of spores of C. botulinum 62A at -75 C was approximately the same as at 20 to 30 C.…”

Section: Discussionmentioning

confidence: 99%

Effect of Temperature of Liquid Nitrogen on Radiation Resistance of Spores of Clostridium botulinum1

Grecz

Snyder

Walker

et al. 1965

Applied Microbiology

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GRECZ, NICHOLAS (U.S. Army Natick Laboratories, Natick, Mass.), 0. P. SNYDER, A. A. WALKER, AND A. ANELLIS. Effect of temperature of liquid nitrogen on radiation resistance of spores of Clostridium botulinum. Appl. Microbiol. 13:527-536. 1965.-An apparatus consisting of a Dewar flask and a relay system controlling the flow of liquid nitrogen permitted the irradiation of samples in tin cans or Pyrex tubes at temperatures ranging from 0 i 1.5 C to -194 i 2 C. An inoculated pack comprising 320 cans of ground beef containing 5 X 104 spores of Clostridium botulinum 33A per can (10 cans per radiation dose) was irradiated with Co6O at 0 and -196 C. Incubation was carried out at 30 C for 6 months. Approximately 0.9 Mrad more radiation was required to inactivate the spores at -196 C than at 0 C. Cans irradiated at -196 C showed partial spoilage at 3.6 Mrad and no spoilage at 3.9 Mrad; the corresponding spoilage-no spoilage doses at 0 C were 2.7 and 3.0, respectively. The majority of positive cans swelled in 2 to 14 days; occasional swelling occurred as late as 20 days. At progressively higher doses, swelling was delayed proportionally to the radiation dose received. The remaining nonswollen cans had no toxin after 6 months of storage, although occasional cans contained very low numbers of viable spores comprising on the average 0.1% of the original spore inoculum.The Dio values in phosphate buffer were 0.290 Mrad for 0 C and 0.396 Mrad for -196 C; in ground beef, the corresponding D1o values were 0.463 Mrad and 0.680 Mrad, respectively. These D,o values indicate that the lethal effect of y rays decreased at -196 C as compared with 0 C by 13.5% in phosphate buffer, and by 47% in ground beef.1.5% agar.These two techniques, i.e., the spoilage endpoint, and the direct count technique, were also used for evaluation of spore survival in ground beef. With the end-point technique in glass tubes, the indications of viability of C. botulinum were turbidity, odor, and specific toxin; with canned beef, swelling of cans and specific toxin were the indicators. 527 on July 5, 2020 by guest http://aem.asm.org/ Downloaded from

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“…Soc. Microbiol., 1972), and it has been shown to be one of the most resistant microorganisms to this sterilizing process (3,19).…”

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confidence: 99%

Resistance and recovery studies on ultraviolet-irradiated spores of Bacillus pumilus

Abshire¹,

Bain²,

Williams³

1980

Appl Environ Microbiol

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A spore suspension model and a procedure for recovering ultraviolet (UV)-irradiated spores of Bacillus pumilus were investigated. A most-probable-number tube dilution method using double-strength Trypticase soy broth was found to be superior to the agar plate method for recovering optimal numbers of spores irradiated with sublethal doses of UV energy. Aqueous suspensions of B. pumilus survived UV doses up to 108,000 ergs/mm2 as determined by a most-probable-number recovery and estimation procedure. Resistance and stability data were consistent and reproducible, indicating the dependability of this method for recovering UV-damaged spores. The procedures used to collect information concerning resistance characteristics for two strains of B. pumilus are discussed.

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Relative Resistances of Microorganisms to Cathode Rays

Abstract: aspects of ionizing radiations as a means of sterilization. Report prepared for Industrial Liaison Conference on Food Sterilization.

Cited by 32 publications

References 4 publications

Influence of Gamma Radiation on the Microflora of Cucumber Fruit and Blossoms123

Influence of Gamma Radiation on the Microflora of Cucumber Fruit and Blossoms123

Effect of Temperature of Liquid Nitrogen on Radiation Resistance of Spores of Clostridium botulinum1

Resistance and recovery studies on ultraviolet-irradiated spores of Bacillus pumilus

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