Relative populations and toxin production by <i>Aspergillus flavus</i> and <i>Fusarium verticillioides</i> in artificially inoculated corn at various stages of development under field conditions

Zorzete, Patrícia; Castro, Rosimar S.; Pozzi, Cláudia Rodrigues; Israel, Ana Lia Martins; Fonseca, Homero; Yanaguibashi, Gianni; Corrêa, Benedito

doi:10.1002/jsfa.2930

Cited by 22 publications

(15 citation statements)

References 31 publications

(26 reference statements)

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“…Contrary, Sreenivasa et al (2011) isolated a high percentage of species, F. verticillioides (86%) and A. flavus (84.9%), from 86 maize kernel samples. According to Zorzete et al (2008) in analysed maize samples the species F. verticillioides was isolated in a greater percentage (34.6%) than the species A. flavus (0.5%) and the correlation coefficient between these two species was negative. Rocha et al (2009) established a statisticaly significant negative correlation (r=-0.61) between isolated Fusarium and Aspergillus genera.…”

Section: Discussionmentioning

confidence: 95%

Impacts of climatic conditions on aflatoxin B1 and fumonisins contamination of maize kernels and their co-occurrence

et al. 2018

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Agroecological and climatic conditions in Serbia greatly affected the development of toxigenic fungi and occurrence of mycotoxins in the maize. The presence of fungal toxigenic Aspergillus and Fusarium species and levels of aflatoxin B 1 (AFB 1) and sum of fumonisins B 1 , B 2 and B 3 (FBs) were established in 127 maize kernel samples harvested during 2012 (37 samples) and 2013 (90 samples). The periods of silking and grain filling of the maize in 2012 in comparison to 2013 were characterised with extremely dry spells, with high temperatures and low precipitation sums. The mean incidences of A. flavus and F. verticillioides were 50.4 and 11.7% in 2012 and 18.9 and 33.4% in 2013, respectively. According to the regulations of the World Health Organisation, unacceptable levels of AFB 1 (>20 μg kg-1) and FBs (>2000 μg kg-1) were established in the 30.6 and 24.1% samples of 2012 and 16.7 and 40% maize kernel samples of 2013, respectively. It can be concluded that high temperatures and low precipitation sums in 2012 favoured the development of A. flavus affecting the high level of AFB 1 , in comparison with F. verticillioides and the production of FBs. There was no positive correlation between the incidences of A. flavus and F. verticillioides, while a statistically significant positive correlation has been found between AFB 1 and FBs levels, in both investigated years (2012-2013). This indicates that the mycotoxin production depended more on weather conditions than on the distribution of corresponding toxigenic fungal species.

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Section: Discussionmentioning

confidence: 95%

Impacts of climatic conditions on aflatoxin B1 and fumonisins contamination of maize kernels and their co-occurrence

et al. 2018

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“…In the natural maize agro-ecosystem, temperatures of 20-30°C are typical between flowering and harvest, and  values of -18.2-2.8 MPa  (0.87-0.98 aw) can occur in maize cobs during early dough to full ripe stage (Zorzete et al, 2008;Battilani et al, 2011). Both pathogen and antagonist can colonize maize substrates at more than -9.8 MPa  (0.93 aw).…”

Section: Discussionmentioning

confidence: 99%

Relationship between environmental conditions, carbon utilisation patterns and Niche Overlap Indices of the mycotoxigenic species Fusarium verticillioides and the biocontrol agent Clonostachys rosea

2016

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Recently, it was shown that a strain of the fungal antagonist Clonostachys rosea 016 was able to inhibit fumonisin B1 mycotoxin production by Fusarium verticillioides FV1 when using different ratios of spores of each species in vitro. The objectives of the present work were therefore to: (a) compare the nutritional utilisation patterns and rates of uptake of key Csources in maize by the antagonist C. rosea 016 and that by the pathogen F. verticillioides FV1; (b) examine their Niche Overlap Indices (NOI) under different interacting environmental conditions; and (c) evaluate whether the rate of utilisation of key maize Csources influenced the competitiveness of either species using the Bioscreen®. It was found that water potential () temperature interactions had significant impacts on C-source utilisation patterns by C. rosea 016 and the pathogen. The NOIs, based on the utilisation of the C-sources by each strain divided by those utilised in common, showed that the antagonist and the pathogen occupied similar niches at -0.70 MPa +30°C and -2.8 MPa +25°C. Under the other conditions tested, they appeared to occupy separate niches suggesting niche exclusion. Temporal C-source utilisation patterns were then compared under different   temperature treatments. This showed that the dominant maize-based C-sources utilised by the pathogen and the antagonist were different. The pathogen F. verticillioides FV1 utilised carbohydrates rapidly followed by amino acids and then one fatty acid, palmitic acid. The antagonist C. rosea 016 utilised both carbohydrates and amino acids at a similar rate but more slowly than the pathogen. There were also differences in the utilisation of some individual amino acids and carbohydrates which might explain the occupation of different niches under some interacting environmental conditions. These findings are discussed in the context of why some competitors are able to inhibit mycotoxin production while others cannot.

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“…Trabalhos realizados no Brasil com grãos de cereais e oleaginosas indicam a presença de aflatoxinas no campo durante diferentes etapas de crescimento das plantas, e apontam fatores como a temperatura e atividade de água como os principais fatores responsáveis pela produção das micotoxinas (Zorzete et al 2008, Rocha et al 2009, Zorzete et al 2011. AFB 1 foi detectada em 14% das 200 amostras analisadas de grãos de milho recém-colhidos em Santa Maria (RS) com níveis variando entre 13,7 a 1.393 µg.kg .…”

Section: Quadro 2 Afb1 Nas Amostras Das Dietas Das Vacas Em Lactaçãounclassified

Estudo sobre a ocorrência de fungos e aflatoxina B1 na dieta de bovinos leiteiros em São Paulo

et al. 2015

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RESUMO.-A qualidade da dieta ofertada às vacas em lactação é uma preocupação dos agentes de saúde devido à possibilidade da detecção de micotoxinas prejudiciais a saúde humana e animal. Os objetivos do trabalho foram avaliar o perfil da micobiota, determinar a atividade de água (Aa) e a ocorrência natural de aflatoxina B 1 (AFB 1 ) em dietas ofertadas a vacas em lactação de fazendas leiteiras no estado de São Paulo, Brasil. As amostragens das dietas foram realizadas diretamente dos cochos de lote de 15 vacas, em dois dias consecutivos com intervalos de 24h e a cada 15 dias, perfazendo um período de 45 dias de amostragens por fazenda. A purificação e determinação de AFB 1 foram realizadas em colunas de imunoafinidade e Cromatografia Líquida de Alta Eficiência (CLAE). O estudo da micobiota presente nas amostras das dietas (288) The quality of the diet offered to lactating cows is a concern to health officials the possibility of detecting mycotoxins harmful to human and animal health. The objectives were to evaluate the profile of mycoflora, determine the water activity (Aw) and the natural occurrence of aflatoxin B 1 (AFB 1 ) in diets offered to lactating cows from dairy farms in the state of São Paulo, Brazil. Samples of the diets were taken directly from the troughs batch of 15 cows, on two consecutive days at intervals of 24 hours and every 15 days with a period of 45 sampling days per farm. Purification and determination of AFB 1 were performed on immunoaffinity columns and High Performance Liquid Chromatography (HPLC). The study of mycobiota present in samples of diets (288) . The water activity of the samples ranged from 0.91 to 0.98. We have detected the presence of AFB 1 in 31.44% of samples with levels between 1.68 a 194.51µg.kg- 1. Measures of good production, storage and use should be taken to reduce the occurrence of aflatoxin B 1 in the diet offered to lactating cow.

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Relative populations and toxin production by Aspergillus flavus and Fusarium verticillioides in artificially inoculated corn at various stages of development under field conditions

Cited by 22 publications

References 31 publications

Impacts of climatic conditions on aflatoxin B1 and fumonisins contamination of maize kernels and their co-occurrence

Impacts of climatic conditions on aflatoxin B1 and fumonisins contamination of maize kernels and their co-occurrence

Relationship between environmental conditions, carbon utilisation patterns and Niche Overlap Indices of the mycotoxigenic species Fusarium verticillioides and the biocontrol agent Clonostachys rosea

Estudo sobre a ocorrência de fungos e aflatoxina B1 na dieta de bovinos leiteiros em São Paulo

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