Relative molecular mass influences the results obtained when determining urinary proteins by laser nephelometry

Wood, W. G.; Steinhoff, Jürgen; Fricke, Harald

doi:10.1016/0022-1759(90)90246-r

Cited by 2 publications

(2 citation statements)

References 3 publications

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“…The higher values obtained by immunonephelometry in the hypertriacylglycerolaemic subgroup could be related to the difference observed between one-site (nephelometry) and two-site (TR-IFMA) assays (23). These higher values could be due to the sensitivity of the nephelometric method to the size of the analyte, owing to differences in light scattering (24). Indeed, the measurement of apolipoprotein B can be inaccurate in samples containing large triacylglycerol-rich particles (12).…”

Section: Discussionmentioning

confidence: 99%

A Sensitive Time-Resolved Immunofluorometric Assay for the Measurement of Apolipoprotein B in Cerebrospinal Fluid. Application to Multiple Sclerosis and Other Neurological Diseases

Osman¹,

Gaillard²,

Meillet³

et al. 1995

CCLM

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Summary: Although low density lipoprotein receptors have been described on oligodendrocytes, apolipoprotein B was thought to be absent or present in only very small amounts in cerebrospinal fluid (CSF). Several immunoassays have been used for the measurement of apolipoprotein B in serum. However, the majority of methods cannot be used to measure small amounts of apolipoprotein B in CSF. In this study, we describe a highly sensitive time resolved immunoftoorometric assay (TR-IFMA) using europium as label (detection limit: 0,3 g/l). The reliability of the TR-IFMA for the measurement of apolipoprotein B was first studied in serum. Serum and CSF apolipoprotein B concentrations were then determined in subjects free of neurological disorders and in patients with multiple sclerosis. Local intrathecal apolipoprotein B synthesis was calculated. Although the high sensitivity of the TR-IFMA allowed low amounts of apolipoprotein B in CSF to be detected (0.11 ± 0.06; 0.12 ± 0.06 mg/1 in controls and multiple sclerosis patients, respectively), no apolipoprotein B could be detected in CSF by electroimmunodiffusion. As suggested by the blood/CSF apolipoprotein B ratio (about 6000), no apolipoprotein B synthesis was observed by both using apolipoprotein B index and formula. This indicates its probable serum origin. Moreover, there was no difference between controls and multiple sclerosis patients in CSF, serum, blood/CSF, index, and local intrathecal apolipoprotein B synthesis. Finally, these results suggest that the role of apolipoprotein B in lipid transport in the central nervous system may be questionable.

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Section: Discussionmentioning

confidence: 99%

A Sensitive Time-Resolved Immunofluorometric Assay for the Measurement of Apolipoprotein B in Cerebrospinal Fluid. Application to Multiple Sclerosis and Other Neurological Diseases

Osman¹,

Gaillard²,

Meillet³

et al. 1995

CCLM

View full text Add to dashboard Cite

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“…Quantitative analysis of urinary proteins was performed using a standard immunoluminometric assay [ 12 , 13 ]. The urinary concentrations of albumin, IgG, and α 1 -microglobulin were measured with an automatic nephelometer (S100; Dade Behring, Schwalbach, Germa ny).…”

Section: Methodsmentioning

confidence: 99%

Proteinuria and the Clinical Course of Dobrava-Belgrade Hantavirus Infection

et al. 2018

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Purpose: Human infection with Dobrava-Belgrade virus (DOBV) in Northern Germany causes a mild form of hantavirus disease predominantly characterized by acute kidney injury due to interstitial nephritis. We evaluated the largest number of DOBV-infected patients so far regarding clinical course, proteinuria, and prognostic markers. Patients and Methods: Patients with DOBV-associated hantavirus disease admitted to the Renal Division of the University of Lübeck (Germany) between 1997 and 2012 were included in this study. Symptoms, clinical course, laboratory parameters, and urinary protein analysis were investigated at admission (baseline, t₀), 3–5 days (t_3–5), 10–17 days (t_10–17), and after 1 year of follow-up (t₃₆₅). Results: Of the 34 patients (male/female ratio: 23/11; age: 41 ± 14 years) included in the study, 4 underwent hemodialysis (HD). Glomerular filtration rate was 17 ± 14 mL/min at t₀ and increased to 27 ± 26 mL/min (t_3–5), 57 ± 20 mL/min (t_10–17), and 84 ± 16 mL/min (t₃₆₅). Albuminuria and tubular proteinuria (α₁- and β₂-microglobulin) decreased during follow-up; the urinary α₁-microglobulin concentration in patients who required HD was significantly higher than that in patients not requiring HD (t₀: 186 ± 51 vs. 45 ± 26 mg/g creatinine; t_3–5: 87 ± 14 vs. 32 ± 16 mg/g creatinine; t_10–17: 63 ± 18 vs. 28 ± 12 mg/g creatinine; p < 0.001). Conclusions: DOBV infection of inpatients in Northern Germany is associated with severe kidney injury that recovers within a few weeks and normalizes within 1 year. Tubular proteinuria is associated with the severity of kidney injury and the necessity of renal replacement therapy in these DOBV-infected patients.

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Relative molecular mass influences the results obtained when determining urinary proteins by laser nephelometry

Cited by 2 publications

References 3 publications

A Sensitive Time-Resolved Immunofluorometric Assay for the Measurement of Apolipoprotein B in Cerebrospinal Fluid. Application to Multiple Sclerosis and Other Neurological Diseases

A Sensitive Time-Resolved Immunofluorometric Assay for the Measurement of Apolipoprotein B in Cerebrospinal Fluid. Application to Multiple Sclerosis and Other Neurological Diseases

Proteinuria and the Clinical Course of Dobrava-Belgrade Hantavirus Infection

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