Relative Frequency of Blastocystis Subtypes 1, 2, and 3 in Urban and Periurban Human Populations of Arequipa, Peru

Ascuña-Durand, Kasandra; Salazar-Sánchez, Renzo; Castillo-Neyra, Ricardo; Ballón-Echegaray, Jorge

doi:10.3390/tropicalmed5040178

Cited by 8 publications

(21 citation statements)

References 53 publications

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“…This study showed that ST3 was the predominant subtype in boys and girls, followed by ST1 and ST2. This result was similar to that of previous studies in Thailand [35,36] and others in different countries, such as Malaysia [37], Turkey [38], Peru [39], and Italy [40]. Our finding showed 18S alleles of Blastocystis in each ST, which include allele 4 (ST1), allele 9 (ST2), and alleles 34 and 37 (ST3).…”

Section: Discussionsupporting

confidence: 92%

Prevalence and Subtype Distribution of Blastocystis Isolated from School-Aged Children in the Thai-Myanmar Border, Ratchaburi Province, Thailand

Abu

Sutthikornchai

Mahittikorn

et al. 2022

IJERPH

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Blastocystis is one of the most common enteric protozoa that inhabits the intestinal tract of humans and different animals. Moreover, it has a worldwide geographic distribution. Its main mode of transmission is via the fecal-oral route. At present, 26 subtypes are widely distributed across both humans and animals. The current study aimed to determine the prevalence and subtype distribution of Blastocystis among school-aged children living on the Thai-Myanmar border, Ratchaburi province, Thailand. In total, 508 samples were collected from children at six schools. The prevalence of Blastocystis infection was amplified and sequenced in the 600 bp barcode region of the small-subunit ribosomal RNA (SSU rRNA). The overall prevalence of Blastocystis infection was 3.35% (17/508). ST3 (11/17) was the most predominant subtype, followed by ST1 (5/17) and ST2 (1/17). A phylogenetic tree was constructed based on the Tamura92+G+I model using the maximum-likelihood algorithm. Surprisingly, all sequences of the ST3-positive samples were closely correlated with the cattle-derived sequence. Meanwhile, all sequences of the Blastocystis ST1-positive samples were closely correlated with the human-derived sequence. Nevertheless, further studies should be conducted to validate the zoonotic transmission of Blastocystis. Based on our findings, personal hygiene and sanitation should be improved to promote better health in children in this area.

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Section: Discussionsupporting

confidence: 92%

Prevalence and Subtype Distribution of Blastocystis Isolated from School-Aged Children in the Thai-Myanmar Border, Ratchaburi Province, Thailand

Abu

Sutthikornchai

Mahittikorn

et al. 2022

IJERPH

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“…However, studies in South America about Blastocystis specifically in animals are scarce. In some South American countries, there are studies reporting different Blastocystis STs in humans and their relationship with symptomatic patients (54)(55)(56)(57)(58). Countries from the Americas where animal samples have been tested from Blastocystis include United States (16,23,29,59), Brazil (25), Mexico (31), Ecuador (35), Peru (34) and Colombia (26).…”

Section: Discussionmentioning

confidence: 99%

Identification of Multiple Blastocystis Subtypes in Domestic Animals From Colombia Using Amplicon-Based Next Generation Sequencing

et al. 2021

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Blastocystis is frequently reported in fecal samples from animals and humans worldwide, and a variety of subtypes (STs) have been observed in wild and domestic animals. In Colombia, few studies have focused on the transmission dynamics and epidemiological importance of Blastocystis in animals. In this study, we characterized the frequency and subtypes of Blastocystis in fecal samples of domestic animals including pigs, minipigs, cows, dogs, horses, goats, sheep, and llama from three departments of Colombia. Of the 118 fecal samples included in this study 81.4% (n = 96) were positive for Blastocystis using a PCR that amplifies a fragment of the small subunit ribosomal RNA (SSU rRNA) gene. PCR positive samples were sequenced by next generation amplicon sequencing (NGS) to determine subtypes. Eleven subtypes were detected, ten previously reported, ST5 (50.7%), ST10 (47.8%), ST25 (34.3%), ST26 (29.8%), ST21 (22.4%), ST23 (22.4%), ST1 (17.9%), ST14 (16.4%), ST24 (14.9%), ST3 (7.5%), and a novel subtype, named ST32 (3.0%). Mixed infection and/or intra -subtype variations were identified in most of the samples. Novel ST32 was observed in two samples from a goat and a cow. To support novel subtype designation, a MinION based sequencing strategy was used to generate the full-length of the SSU rRNA gene. Comparison of full-length nucleotide sequences with those from current valid subtypes supported the designation of ST32. This is the first study in Colombia using NGS to molecularly characterize subtypes of Blastocystis in farm animals. A great diversity of subtypes was observed in domestic animals including subtypes previously identified in humans. Additionally, subtype overlap between the different hosts examined in this study were observed. These findings highlight the presence of Blastocystis subtypes with zoonotic potential in farm animals indicating that farm animals could play a role in transmission to humans.

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“…Parts of the metabarcoding data used in the present study had been generated for various independent research projects and routine diagnostic purposes and were recently used in a separate study (Stensvold et al, 2022). Data representing fecal samples collected in Peru and Tunisia (Ascuna-Durand et al, 2020;Chihi et al, 2019Chihi et al, , 2022 were added. Thus, the 1457 fecal DNA datasets represented populations sampled in Algeria, Denmark, Egypt, Peru, Tunisia, Turkey, and United Kingdom, with and without gastrointestinal symptoms.…”

Section: At Er I a L S A N D M Et Hod S Datamentioning

confidence: 99%

Further insight into the genetic diversity of Entamoeba coli and Entamoeba hartmanni

Stensvold

Ascuña-Durand

Chihi

et al. 2022

J Eukaryotic Microbiology

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Despite the species' wide distribution, studies of the genetic diversity within Entamoeba coli and Entamoeba hartmanni remain limited. In the present study, we provide further insight into the genetic diversity of both species based on analysis of partial nuclear small subunit ribosomal DNA sequences generated from human faecal DNAs from samples collected in Africa, South America and Europe. Reinforcing the previous recognition that E. coli is a species complex, our data confirm the existence of the two subtypes, ST1 and ST2, previously identified plus, potentially, a new subtype, ST3. While ST1 appears to be genetically quite homogenous, ST2 shows a substantial degree of intra-subtype diversity. ST2 was more common in samples collected outside Europe, whereas ST1 showed no geographical restriction. The potentially novel subtype is represented to date exclusively by sequences from South American and African samples. In contrast to previous reports, our new data also indicate substantial variation in E. hartmanni that could also support the establishment of subtypes within this species. Here, however, no links were identified between subtype and geographical origin.This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process which may lead to differences between this version and the Version of Record. Please cite this article as

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Relative Frequency of Blastocystis Subtypes 1, 2, and 3 in Urban and Periurban Human Populations of Arequipa, Peru

Cited by 8 publications

References 53 publications

Prevalence and Subtype Distribution of Blastocystis Isolated from School-Aged Children in the Thai-Myanmar Border, Ratchaburi Province, Thailand

Prevalence and Subtype Distribution of Blastocystis Isolated from School-Aged Children in the Thai-Myanmar Border, Ratchaburi Province, Thailand

Identification of Multiple Blastocystis Subtypes in Domestic Animals From Colombia Using Amplicon-Based Next Generation Sequencing

Further insight into the genetic diversity of Entamoeba coli and Entamoeba hartmanni

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