This is the first report of the potential of Acacia fast growing trees in Thailand, A. mangium and the Acacia hybrid (A. mangium x A. auriculiformis), as raw material for ethanol production through a simultaneous saccharification and fermentation process by Saccharomyces cerevisiae TISTR 5339. Alkaline pulping was applied as the pretreatment process. Optimization of ethanol production was studied using response surface methodology based on central composite design. The optimized conditions of 100 g/L solid loading and an A600 of S. cerevisiae TISTR 5339 of 2 gave observed values of ethanol production of 35.7 and 27.3 g/L, which corresponded with the predicted values of 32.32 and 26.37g/L from A. mangium and A. hybrid, respectively. This condition was then used for up-scaling in a 10-L stirred bioreactor. The improved maximum ethanol concentrations of 37.84 and 36.52 g/L were obtained from A. mangium and Acacia hybrid, respectively, within 96 h of cultivation at 30 °C and no aeration rate.
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INTRODUCTIONWith the depletion of the world's petroleum, alternative non-petroleum-based sources of energy are being looked at with greater interest. Many countries have begun focusing on renewable resources for production of ethanol-based fuels. Lignocellulosic biomass from woody plants has the potential to become a major source of fermentable sugars for the production of ethanol because trees are the most abundant source of biomass. Thus, lignocellulosic biomass has been considered a new resource for ethanol production.Because of its chemical composition, lignocellulosic biomass is very different from types of biomass that have large content of sugars or starch, as is customarily used in the biofuel industry. The structure of these former materials, mainly composed by cellulose, hemicellulose, and lignin, requires the process for biofuels production to be adjusted for each type of biomass, according to their component characteristics. Thus, pretreatment has been recognized as a necessary upstream process to improve the formation of sugars for downstream microbial and enzymatic processing.Different kind of pretreatment methods, under a large variety of conditions, have been studied to improve the fermentability and digestibility. Mechanical size reduction is a physical pretreatment to increase enzyme-accessible surface areas (Zhu et al. 2009). In addition to these other pretreatment options, steam explosion is a technique based on PEER-REVIEWED ARTICLE bioresources.com Boondaeng et al. (2015). "Ethanol from Acacia," BioResources 10(2), 3154-3168. 3155 subjecting the biomass to pressurized steam for a short duration of time and then suddenly depressurizing the system. Due to the explosive decomposition, the fibers are separated and changes to the microstructure are brought about by the suddenly reduced pressure (Brodeur et al. 2011). The treatments result in increasing cellulose digestibility of pretreated biomass and solubilizing a significant portion of the hemicellulosic component (Nibedita et al. 2012). To help wi...