Relative Effectiveness of Iron in Complex with Organic Ligands on Functional Iron Status of Maize Plants

A comparative study on the efficacy of synthetic organic ligands on functional iron status in plants

“…Fe-EDDHA is more suitable than FeCl 3 under hydroponics and might be a relatively less-poorer Fe-ligand for wheat. Fe-EDDHA is the poorest Fe-ligand for maize plants (Gupta et al 2024).…”

“…PS exhibited a negative correlation with shoot Fe concentration, as Fe-EDDHA-treated plants experienced Fe de ciency and thus, they produced more PS. Likewise, Fe-EDDHA lies in the negative coordinates of PC1 and PC2 for maize plants (Gupta et al 2024).…”

“…Wheat plants were treated with 25 µM FeCl 3 (control) or Fe-ligands, viz., 25 µM Fe 3+ -EDTA (Feethylene diaminetetraacetic acid), 50 µM Fe 3+ -EDTA, 25 µM Fe 3+ -EDDHA (Fe-ethylenediamine-N, N' bis (o-hydroxyphenyl) acetic acid), and 25 µM Fe 3+ -citrate and were grown under hydroponic culture conditions (photoperiod 14 h dark/10 h light at 35°C, 75% relative humidity, and 200 µmol m − 2 s − 1 light intensity). Maize plants were also grown under similar conditions but at a different supply of Fe-ligands (150 µM) as described earlier (Gupta et al 2024). The nutrient solution was comprised of 2 mM KNO 3 ; 2.0 mM Ca(NO 3 ) 2 ; 1.0 mM MgSO 4 ; 0.65 mM NaH 2 PO 4 ; 0.05 mM NaCl; 5.0 µM MnSO 4 ; 0.5 µM CuSO 4 ; 0.5 µM ZnSO 4 ; 15 µM H 3 BO 3 ; 0.1 µM Na 2 MoO 4 ; 0.05 µM CoSO 4 ; and 0.05 µM NiSO 4 (Hewitt 1966).…”

“…The oven-dried shoots and root samples were digested in nitric acid and perchloric acid (10:1 v/v mixture) as described earlier (Gupta et al 2024). Fe-bipyridyl (10 mM) complex formed in the presence of ascorbic acid (200 mM) were measured at 520 nm using a double-beam UV-Vis spectrophotometer.…”

“…Wheat plants have been considered more Fe-e cient than maize. Therefore, wheat and maize were supplied with Feligands (Fe-EDTA, Fe-EDDHA, and Fe-Citrate) at 25 µM and 150 µM, respectively (Gupta et al 2024). In this work, we aimed to provide a comparative insight of wheat with maize following differences in functional Fe entities (total chlorophyll, shoot Fe concentration, Fe translocation e ciency, ferric chelate reductase activity, phytosiderophore production, and lipid peroxidation).…”