Relative Contribution of Cytochrome-mediated and Cyanide-resistant Electron Transport in Fresh and Aged Potato Slices

Theologis, Athanasios; Laties, George G.

doi:10.1104/pp.62.2.232

Cited by 135 publications

(105 citation statements)

References 23 publications

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“…The engagement of the alternative path reflects the inability of the Cyt path to accommodate the total enhanced flux due to aging. Maximal Kyt values (measured in the presence of uncoupler) in fresh and aged potato slices are approximately the same (28,29). Accordingly, the elevated observable Cyt-mediated rates of aged slices are not to be attributed to the de novo synthesis of Cyt components and, hence, of mitochondria.…”

Section: Slice Aging and Cn Resistancementioning

confidence: 76%

“…the mitochondria (1). It seems that aging activates and utilizes pre-existing respiratory capacity without the biogenesis of new mitochondria (28,29).…”

Section: Biochemicalsmentioning

confidence: 99%

“…The contribution of the alternative path of the wound and wound-induced respiration was estimated (2,28,29), as well as its magnitude, to establish the extent of its participation. …”

Section: Introductionmentioning

confidence: 99%

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Membrane Lipid Breakdown in Relation to the Wound-induced and Cyanide-resistant Respiration in Tissue Slices

Theologis¹,

Laties²

1980

Plant Physiol.

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A study of a variety of bulky storage organs and fruits reveals that fresh slices fall into two categories with respect to their sensitivity to CN. Fresh slices in the first class are CN-sensitive, whereas slices of the second class are resistant to, and often stimulated by, CN alous (17). Their CN sensitivity has been attributed to the loss of the alternative path (27) due to extensive membrane lipid destruction initiated by slicing. Furthermore, regeneration of the CNresistant pathway, and the burgeoning of glycolysis and the tricarboxylic acid cycle (12,14) in aged potato slices were viewed as the result of specific membrane biosynthesis (33,34). The question arises whether these physiological transitions are ubiquitous in tissue slices or unique to the potato slice.Here, tissue slices from a variety of bulky storage organs and fruits were examined to determine whether slicing in general initiates a burst of lipolytic activity analogous to that found in potato and how slicing affects CN resistance and the utilization of exogenous glucose. The contribution of the alternative path of the wound and wound-induced respiration was estimated (2,28,29), as well as its magnitude, to establish the extent of its participation. MATERIALS AND METHODS PLANT

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Section: Slice Aging and Cn Resistancementioning

confidence: 76%

“…the mitochondria (1). It seems that aging activates and utilizes pre-existing respiratory capacity without the biogenesis of new mitochondria (28,29).…”

Section: Biochemicalsmentioning

confidence: 99%

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Membrane Lipid Breakdown in Relation to the Wound-induced and Cyanide-resistant Respiration in Tissue Slices

Theologis¹,

Laties²

1980

Plant Physiol.

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“…The pieces were placed in a cuvette and the respiration was estimated by oxygen consumption (mmol O 2 g -1 FW h -1 ) with the aid of an electrode of Clark-type at following temperatures: 10°, 20°, 25° and 35° C. Activity of the alternative respiratory pathway (v alt ) was studied using method of specific inhibitors (BAHR and BONNER, 1973;THEOLOGIS and LATIES, 1978) and was estimated as the difference between total respiration (without inhibitors) and respiration in the presence of benzhydroxamic acid (BHAM, 25.0 mmol). All experiments were carried out in triplicate.…”

Section: Methodsmentioning

confidence: 99%

Temperature-dependent alterations of respiration in leaves of two selected vascular plant species: the role of the alternative oxidase pathway

Pystina

Danilov

2002

Bragantia

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Effects of higher temperatures on respiration and activity of alternative oxidase (AOX) were studied in mature leaves of Ajuga reptans L. and Rhodiola rosea L. Total respiration in both A. reptans and R. rosea increased exponentially with the increasing temperature of 10 °C to 35 °C. Respiration in the presence of benzhydroxamic acid (BHAM) also increased exponentially in accordance with the increasing temperature in the leaves of both A. reptans and R. rosea. Relative activity of the alternative pathway decreased significantly in the leaves of A. reptans with increasing temperatures. However, an increase in the relative activity of the alternative pathway was detected in the leaves of R. rosea. Thermoresistance of the alternative respiratory pathway was considered to be higher in R. rosea compared to A. reptans. We suppose that A. reptans and R. rosea have different mechanisms regulating partitioning of electrons to the alternative respiratory pathway. Key words: alternative respiratory pathway, respiration, alternative oxidase, leaves, temperature. RESUMO ALTERAÇÕES DE RESPIRAÇÃO DEPENDENTES DE TEMPERATURA EM FOLHAS DE DUAS ESPÉCIES DE PLANTAS VASCULARES SELECIONADAS: O PAPEL DO CAMINHAMENTO ALTERNATIVO DA OXIDASEEstudaram-se os efeitos de temperaturas mais altas na respiração e na atividade do caminhamento alternativo da oxidase (AOX) em folhas maduras de Ajuga reptans L. e de Rhodiola rosea L. Verificou-se que a respiração total, tanto em A. reptans como em R. rosea, apresentou aumento exponencial com a temperatura crescente de 10 ºC para 35 ºC. Observou-se, também, que na presença de ácido benzidroxâmico (BHAM) houve aumento exponencial na respiração com a variação crescente da temperatura nas folhas das duas plantas estudadas. A atividade relativa do caminhamento alternativo diminuiu significativamente nas folhas de A. reptans com temperaturas crescentes; verificou-se, porém, aumento nas folhas de R. rosea. A termorresistência do caminhamento respiratório alternativo foi considerada mais alta em R. rosea que em A. reptans. Supõe-se que ambas possuem diferentes mecanismos que regulam a partição de elétrons para o caminhamento respiratório alternativo. Palavras-chaves: caminhamento respiratório alternativo, respiração, oxidase alternativo, folhas, temperatura.( 1 )

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“…Aging of the sections brings on further increases in the rate ofrespiration which is then principally cyanide-resistant (I 1). In each case, the cyanide-resistant respiration remains unexpressed in the absence of metabolic inhibitors (12). Ultrastructural changes accompany sectioning (13) breakdown products (6).…”

mentioning

confidence: 99%

Wounding Stimulates Cyanide-sensitive Respiration in the Highly Cyanide-resistant Leaves of Bryophyllum tubiflorum Harv.

Kinraide¹,

Marek²

1980

Plant Physiol.

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The rate of respiration in sectioned leaves of Bryophylim tubiflorwm Harv. increases with decreasing section thickness. The rates of uninhibited respiration in 2-and 8-millimeter-thick sections are 74 and 46 microliters of 02 per gram fresh weight of unmptured tissue per hour at 20 C, whereas the rate in the presence of cyanide is 31 microliters of 02 in each case.Te rates are unaffected by salicylhydroxamic acid, but cyanide and salicylbydroxamic acid together completely eliminate 02 uptake. The capacity of the alternative respiratory pathway is thus initially high (estimated at 84% of the uninhibited respiratory rate in whole leaves) and remains constant but probably unexpressed subsequent to the rapid induction of wound respiration.When the succulent leaves of Bryophyllum tub(florum Harv. are cut into transverse sections the rate of respiration increases in a reciprocal relationship to sec-tion thickness. The respiratory rise is rapidly induced and is always observed at the first measurement. Subsequently, the rate in the thinner sections declines somewhat over a 3-to 4-h period, then remains constant for many additional hours. The rates, even in thick sections, are unaffected by the concentration of external 02 between 5 and 00%o or by the presence (1%) or absence of CO2 (5). Little more is known of wounding phenomena in Bryophyllum leaves except that wound respiration inhibits dark accumulation of malic acid (5). In order to characterize the wound respiration further we have determined the effect of sectioning upon cyanide-resistant respiration.Our interest in cyanide-resistant respiration was inspired by the effect of sectioning upon potato tuber tissue. Whole tubers respire at low rates and are cyanide-resistant (11). Fresh slices experience a sharp increase in respiration that is predominantly cyanidesensitive unless the tubers from which the sections were taken had been aerobically treated with ethylene (10) or one of several other compounds (3). Aging of the sections brings on further increases in the rate ofrespiration which is then principally cyanide-resistant (I 1). In each case, the cyanide-resistant respiration remains unexpressed in the absence of metabolic inhibitors (12). Ultrastructural changes accompany sectioning (13), and the substrates for the immediate 5-fold increase in respiration are the membrane-lipid

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Relative Contribution of Cytochrome-mediated and Cyanide-resistant Electron Transport in Fresh and Aged Potato Slices

Cited by 135 publications

References 23 publications

Membrane Lipid Breakdown in Relation to the Wound-induced and Cyanide-resistant Respiration in Tissue Slices

Membrane Lipid Breakdown in Relation to the Wound-induced and Cyanide-resistant Respiration in Tissue Slices

Temperature-dependent alterations of respiration in leaves of two selected vascular plant species: the role of the alternative oxidase pathway

Wounding Stimulates Cyanide-sensitive Respiration in the Highly Cyanide-resistant Leaves of Bryophyllum tubiflorum Harv.

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