Relative comparison of tissue specific bioaccumulation and radiation dose estimation in marine and freshwater bivalve molluscs following exposure to phosphorus-32

Vernon, Emily L.; Smith, Jim T.; Jha, Awadhesh N.

doi:10.1016/j.jenvrad.2018.07.005

Cited by 10 publications

(15 citation statements)

References 57 publications

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“…Four 10-day Cu exposure experiments were performed between April 2016 and December 2017, the first two to determine genotoxic responses and the latter for tissue specific Cu accumulation measurements. Adult MG (shell length 44.5 ± 6.5 mm) and DP (shell length 26.7 ± 4.31 mm) were collected from Trebarwith strand (as in Dallas et al [4][5]) and Bude, Cornwall, UK (50.828059, -4.549053), respectively (Vernon et al [37]). DP was maintained in an artificial river water solution (2M CaCl2.2H20, 8 mM MgSO4.7H2O, 40 mM, 5 mM KNO3, 0.7 M NaHCO3), MG in filtered (< 10 µM), aerated natural seawater.…”

Section: Mussel Exposure Conditionsmentioning

confidence: 99%

“…DP was maintained in an artificial river water solution (2M CaCl2.2H20, 8 mM MgSO4.7H2O, 40 mM, 5 mM KNO3, 0.7 M NaHCO3), MG in filtered (< 10 µM), aerated natural seawater. Mussels were fed a solution of Isochrysis galbana algae (MG; Reed Mariculture, Campbell, CA, USA) or dried Chlorella powder, two hours prior to water changes and were kept at a 12:12 photoperiod at 15 °C as described in earlier studies from our laboratory [5,37].…”

Section: Mussel Exposure Conditionsmentioning

confidence: 99%

“…It is well accepted that following uptake, waterborne contaminants are not uniformly distributed among the tissues due to their inherent metabolic capabilities [4,17,37,[71][72][73][74].…”

Section: Tissue-specific Cu Accumulationmentioning

confidence: 99%

“…Species-specific differences in bioaccumulation and resultant biological response following exposure to environmental contaminants have been reported for different marine bivalves [4,32,[35][36]. Despite the fact that (a) freshwater and marine bivalves are comparable in their external and internal anatomical structures [37] and (b) contaminants including metals are highly relevant to both marine and freshwater environments, there have been no attempts to compare the relative bioaccumulation and biological responses in these ecologically relevant organisms. The marine, Mytilus galloprovincialis (MG) and freshwater mussel, Dreissena polymorpha (DP) were chosen for this study as ecologically relevant representatives of both coastal and inland water bodies [37][38][39].…”

Section: Introductionmentioning

confidence: 99%

“…Despite the fact that (a) freshwater and marine bivalves are comparable in their external and internal anatomical structures [37] and (b) contaminants including metals are highly relevant to both marine and freshwater environments, there have been no attempts to compare the relative bioaccumulation and biological responses in these ecologically relevant organisms. The marine, Mytilus galloprovincialis (MG) and freshwater mussel, Dreissena polymorpha (DP) were chosen for this study as ecologically relevant representatives of both coastal and inland water bodies [37][38][39]. As sessile, filter feeding organisms, both species are used extensively in monitoring programmes and in toxicological research as accurate bioindicators of their environmental surroundings [40].…”

Section: Introductionmentioning

confidence: 99%

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Assessing relative sensitivity of marine and freshwater bivalves following exposure to copper: Application of classical and novel genotoxicological biomarkers

Vernon

Jha

2019

Mutation Research/Genetic Toxicology and Environmental Mutagene

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Determination of relative sensitivity of biota following exposures to contaminants including metals is important for environmental protection. Copper (Cu), although biologically essential can be highly toxic to biota if present at higher concentrations in the natural environment. Given its ubiquitous presence within coastal and inland water bodies, we compared Cu-induced genotoxicity in two ecologically important mussel species, the freshwater Dreissena polymorpha (DP) and marine Mytilus galloprovincialis (MG), along with its tissue specific accumulation. Novel biomarker in terms of induction of gamma H2AX (γ-H2AX) foci, along with comet assay and induction of micronuclei (MN) were used to determine DNA damage response (DDR) in these two species following exposure to a range of Cu concentrations (18, 32, 56 µg L-1) for 10 days. Concentration-dependent increases in Cu concentration in gill tissue, as determined by Inductively Coupled Plasma Mass Spectrometry (ICP-MS), were paralleled by a greater degree of genotoxicity. An induction of γ-H2AX foci was present in all Cu exposure concentrations, proving this technique to be a sensitive and suitable biomarker of genotoxicity in bivalves. The multibiomarker approach adopted here suggests firstly that in parallel with MG, which is widely used to assess the health of marine and coastal environment, DP is also suitable representative of inland water bodies, and that there is a similar mechanism of action for the induction of genotoxicity between the two species, following exposure to Cu. Secondly, for genotoxicity assessment a battery of responses could simultaneously be assessed in these two bivalve species. Finally, for adequate protection of the environment it is vital to adopt a multi-biomarker, multi-species approach to determine adverse biological effects to gain a holistic understanding of the real threat posed by contaminants to hydrosphere.

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Section: Mussel Exposure Conditionsmentioning

confidence: 99%

Section: Mussel Exposure Conditionsmentioning

confidence: 99%

“…It is well accepted that following uptake, waterborne contaminants are not uniformly distributed among the tissues due to their inherent metabolic capabilities [4,17,37,[71][72][73][74].…”

Section: Tissue-specific Cu Accumulationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Assessing relative sensitivity of marine and freshwater bivalves following exposure to copper: Application of classical and novel genotoxicological biomarkers

Vernon

Jha

2019

Mutation Research/Genetic Toxicology and Environmental Mutagene

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2022

Marine Radioecology

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Genotoxic and epigenetic effects of diuron in the Pacific oyster: in vitro evidence of interaction between DNA damage and DNA methylation

Akcha

Barranger

Bachère

2020

Environ Sci Pollut Res

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Recently, research has contributed to better knowledge on the occurrence of pesticides in coastal water by identifying frequently detected substances, their concentration range and their acute and chronic toxicity for organisms. Pesticide pollution is of particular concern in France due to important agricultural activities and presence of several exoreic catchment areas that vehicle pesticides up to coastal waters, impacting non-target marine species. Several ecotoxicology questions remain to be addressed concerning the long-term effects of chronic pesticide exposure and the mechanisms involved in adaptation to chemical stress. In the present study, we brought new insights on the genetic and epigenetic effects of the herbicide diuron in oyster genitors. During gametogenesis, we exposed Crassostrea gigas to environmentally realistic herbicide concentrations (0.2-0.3 μg L−1 during two 7-day periods at half-course and end of gametogenesis). Diuron exposure was shown to decrease global DNA methylation and total methyltransferase activity in whole oyster tissue; this is consistent with the previous observation of a significant decrease in DNMT1 gene expression. Diuron effect seemed to be tissue-specific; hypermethylation was detected in the digestive gland, whereas diuron exposure had no effect on gill and gonad tissue. The genotoxicity of diuron was confirmed by the detection of one adduct in gonad DNA. By using in vitro approaches and human DNMT1 (DNMT1 has not been purified yet in bivalves), the presence of DNA lesions (adduct, 8-oxodGuo) was shown to interfere with DNMT1 activity, indicating a complex interaction between DNA damage and DNA methylation. Based on our results, we propose mechanisms to explain the effect of diuron exposure on DNA methylation, a widespread epigenetic mark.

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Relative comparison of tissue specific bioaccumulation and radiation dose estimation in marine and freshwater bivalve molluscs following exposure to phosphorus-32

Cited by 10 publications

References 57 publications

Assessing relative sensitivity of marine and freshwater bivalves following exposure to copper: Application of classical and novel genotoxicological biomarkers

Assessing relative sensitivity of marine and freshwater bivalves following exposure to copper: Application of classical and novel genotoxicological biomarkers

References

Genotoxic and epigenetic effects of diuron in the Pacific oyster: in vitro evidence of interaction between DNA damage and DNA methylation

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