Relative charge density model on chitosan–fucoidan electrostatic interaction: Qualitative approach with element analysis

Formation of chitosan-fucoidan nanoparticles and their electrostatic interactions: Quantitative analysis

“…Chitosanfucoidan complex nanoparticles were prepared using the methodology reported elsewhere (26). A 0.1 %w/v chitosan solution was prepared to a final volume of 100 ml by dissolving 100 mg of chitosan (deacetylation degree of 75e85%, viscosity (0.5% in 5% acetic acid) of 5e20 cps, case no.…”

“…The pH of the chitosan solution before and after adding the fucoidan solution was measured using a methodology reported elsewhere (26). The unadjusted pH of the 0.1 %w/v chitosan solution (in 0.2 %w/v acetic acid) and 0.1 %w/v fucoidan solution was 3.69 and 7.08, respectively.…”

“…On the other hand, few studies have correlated the experimental data on the characterization and the formation of CFNs with the extent of the ionic interactions between chitosan and fucoidan using model-approach-analysis. Under these circumstances, a previous study (26) proposed a relative charge density model of prepared CFNs to provide insight into an analysis of the ionic interactions in terms of the polyelectrolyte complexes. Using the proposed relative charge density model, the extent of the ionic interactions can be predicted in terms of the pH (pH 2e6) and the fucoidan to chitosan mass ratio (FCMR) (1:0.05 through 1:1), where positively net charged CFNs were prepared.…”

“…In addition to the previous study (26), the formation of CFNs and their electrostatic interactions still need to be analyzed quantitatively. In the present study, the stoichiometric distributions of both positive amino groups and negative sulfate ions loaded in CFNs were obtained by deriving the expression of their loaded concentrations from the experimental data.…”

“…In the present study, the stoichiometric distributions of both positive amino groups and negative sulfate ions loaded in CFNs were obtained by deriving the expression of their loaded concentrations from the experimental data. In addition, the relative charge density model suggested previously (26) was applied to predict the distribution of both positive amino groups and negative sulfate ions loaded in CFNs in terms of the pH and FCMRs. The prediction was carried out quantitatively by correlating the stoichiometry of the amino groups and sulfate groups loaded in the CFNs, and the relative charge density model.…”