Relative Abundance of Human Bocaviruses in Urban Sewage in Greater Cairo, Egypt

Hamza, Hazem; Leifels, Mats; Wilhelm, Michael; Hamza, Ibrahim Ahmed

doi:10.1007/s12560-017-9287-3

Cited by 34 publications

(27 citation statements)

References 39 publications

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“…However, increasing data suggest a role for this virus, at least for HBoV-2, as an etiological agent of gastroenteritis in the pediatric population (25). HBoV can be discharged in untreated wastewaters through virus shedding and, consequently, disperse throughout water environments, as shown recently in Italy (30,31) and elsewhere (26,28,32). Bivalve mollusks concentrate several human pathogens transmitted by the fecal-oral route, including a wide range of enteric viruses (35)(36)(37), whose presence in these aquatic organisms may be evaluated to assess the safety of the product for human consumption (38) or for biomonitoring purposes (39,40).…”

Section: Resultsmentioning

confidence: 98%

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Detection of Human Bocavirus Species 2 and 3 in Bivalve Shellfish in Italy

Rosa

Purpari

Guercio

et al. 2018

Appl Environ Microbiol

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Human bocavirus (HBoV) has been shown to be a common cause of respiratory infections and gastroenteritis in children. Recently, HBoVs have been detected in sewage and river waters in Italy and worldwide. However, studies on their presence in other water environments and in bivalve mollusks are not yet available. In this study, 316 bivalve shellfish samples collected in three Italian regions over a 6-year period (2012 to 2017) were analyzed by nested PCR and sequencing using broad-range primer pairs targeting the capsid proteins VP1 and VP2 of HBoV. The virus was detected in 27 samples (8.5% of the total samples), and a statistically significant difference was found within the three regions. A further 13 samples, collected in geographic and temporal proximity to positive samples, were included in the study to assess the spread of HBoV in shellfish production areas at the time of contamination. Twelve of these additional samples were found to be positive for HBoV. All positive samples in this study were characterized as HBoV species 2 (17 samples; 8 different sequences) or species 3 (22 samples; 4 different sequences). This study reports the occurrence of HBoV in bivalve shellfish and shows evidence of considerable spatial spread of the virus throughout shellfish production areas. Further studies are needed to elucidate both the role of HBoV as an agent of gastroenteritis and the risk for foodborne transmission of this virus. Human bocavirus is recognized as an important cause of acute respiratory tract infections and has recently been considered an etiological agent of gastroenteritis in the pediatric population. Our findings document that HBoVs are detected in bivalve shellfish with a relevant prevalence and suggest that an assessment of the risk for foodborne transmission of these viruses should be undertaken.

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Section: Resultsmentioning

confidence: 98%

“…Recently, the presence of HBoV in environmental samples, including wastewaters, sewage sludge, and water impacted by sewage, has also been reported (26)(27)(28)(29)(30)(31)(32)(33), therefore suggesting a potential role of water in the transmission of this virus. To date, no information on the presence of HBoV in food matrices is available.…”

mentioning

confidence: 97%

Detection of Human Bocavirus Species 2 and 3 in Bivalve Shellfish in Italy

Rosa

Purpari

Guercio

et al. 2018

Appl Environ Microbiol

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“…There are currently limited data worldwide exploring the circulation of HBoV from environmental samples. Some studies have detected human bocavirus in river water [25,26] and wastewater samples [27][28][29]. Even though the role of HBoV in gastrointestinal infections is not fully understood, the risk of infection via contaminated water should be taken into consideration since many rural communities still face challenges of poor sanitation and hygiene practices [15].…”

Section: Discussionmentioning

confidence: 99%

Predominance of Human Bocavirus Genotype 1 and 3 in Outpatient Children with Diarrhea from Rural Communities in South Africa, 2017–2018

et al. 2020

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Human bocavirus (HBoV) is an emerging virus globally associated with diarrhea in young children. This study aims to investigate the prevalence of HBoV genotypes in children (≤5 years) from rural communities in South Africa (SA) suffering from acute gastroenteritis (AGE). A total of 141 fecal samples of children ≤5 years with acute gastroenteritis (AGE) were collected from rural primary health care facilities in the Vhembe district of SA between June 2017 and July 2018. Clinical symptoms and demographic data were also recorded. A total of 102 (72%) were outpatients, and 39 (28%) were hospitalized patients. Human bocavirus (HBoV) genotypes were determined using real-time multiplex PCR. DNA extracts of positive samples were confirmed by conventional PCR targeting the NS1 gene. Co-infection with other enteric viruses were determined in HBoV-positive samples using real-time PCR. HBoV was detected in eight (5.7%) children with AGE, of which three (37.5%) were HBoV1, three (37.5%) were HBoV3, and two (25%) were HBoV2. The majority of positive cases were identified in outpatients (62%) between the ages of 1 and 24 months. Co-infection in HBoV-positive samples with other enteric viruses included rotavirus (37.5%), adenovirus (37.5%), norovirus (25%), and astrovirus (12.5%). HBoV infections could be seen as a potential emerging diarrheal pathogen in South Africa. However, more studies are needed to understand the role of HBoV infections in children with AGE.

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“…There is currently limited data worldwide exploring the circulation of HBoV from environmental samples. Some studies have shown the prevalence of Human Bocavirus in river water (22,23) and wastewater samples (24)(25)(26). Even though the role of HBoV in gastrointestinal infections still remains to be not fully understood, the risk of infection via contaminated water should be taken into consideration since many rural communities still face challenges of poor sanitation and hygiene practices (11).…”

Section: Discussionmentioning

confidence: 99%

Human Bocavirus Prevalence In Children With Acute Gastroenteritis From Rural Communities In The Northen Region Of South Africa

Rikhotso

Khumela

et al. 2019

Preprint

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BACKGROUND: Acute gastroenteritis (AGE) is a leading cause of morbidity and mortality in young children worldwide. Human Bocavirus (HBoV) is an emerging virus globally associated with diarrhea. The aim of this study was to demonstrate the prevalence of HBoV genotypes in children (≤5 years) from rural communities in South Africa (SA) suffering from AGE. MATERIAL AND METHOD:A total of 141 fecal samples of children ≤5 years with acute gastroenteritis (AGE) were collected from rural Primary Health Care facilities in the Vhembe district of SA between June 2017 and July 2018. Clinical symptoms and demographic data were also recorded. A total of 102 (72%) were outpatients and 39 (28%) were hospitalized patients. Human Bocavirus (HBoV) genotypes were determined using Real-Time Multiplex PCR. DNA extracts of positive samples were confirmed by conventional PCR targeting the NS1 gene. Co-infection with other enteric viruses were determined in HBoV positive samples using Real-Time PCR. RESULTS: HBoV was detected in 8 (5.7%) children with AGE. Children were in the age group between 1-24 months. HBoV1 and HBoV3 genotypes were each detected in 3 (37.5%) stool samples and HBoV2 in 2 (25%) stool samples. Co-infection with other enteric viruses included Rotavirus (37.5%); Adenovirus (37.5%); Norovirus (25%) and Astrovirus (12.5%). CONCLUSION: HBoV infections could be seen as a potential emerging diarrheal pathogen in South Africa. Further studies are required to understand the role of HBoV infections in children and adults with acute gastroenteritis.

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Relative Abundance of Human Bocaviruses in Urban Sewage in Greater Cairo, Egypt

Cited by 34 publications

References 39 publications

Detection of Human Bocavirus Species 2 and 3 in Bivalve Shellfish in Italy

Detection of Human Bocavirus Species 2 and 3 in Bivalve Shellfish in Italy

Predominance of Human Bocavirus Genotype 1 and 3 in Outpatient Children with Diarrhea from Rural Communities in South Africa, 2017–2018

Human Bocavirus Prevalence In Children With Acute Gastroenteritis From Rural Communities In The Northen Region Of South Africa

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