Relative abundance and localization of interferon-stimulated gene 15 mRNA transcript in intra- and extra-uterine tissues during the early stages of pregnancy in sheep

Alak, Ilyas; Hitit, Mustafa; Köse, Mehmet; Kaya, Muhsin; Uçar, Eyyüp Hakan; Atli, Zehra; Atlı, Mehmet Osman

doi:10.1016/j.anireprosci.2020.106347

Cited by 12 publications

(7 citation statements)

References 25 publications

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“…FISH analysis was performed to determine the circ_MYRF location in the endometrium tissues of P16 and C16 ( Figure 9 ). It is abundantly expressed in the uterine glandular epithelium (GE) and stroma, which is consistent with ISG15 expression in the endometrial tissue, as reported in a previous study [ 29 ].…”

Section: Resultssupporting

confidence: 89%

Integrating Analysis to Identify Differential circRNAs Involved in Goat Endometrial Receptivity

Wang

Zang

et al. 2023

IJMS

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Endometrial receptivity is one of the main factors underlying a successful pregnancy, with reports substantiating the fact that suboptimal endometrial receptivity accounts for two-thirds of early implantation event failures. The association between circRNAs and endometrial receptivity in the goat remains unclear. This study aims to identify potential circRNAs and regulatory mechanisms related to goat endometrial receptivity. Therefore, the endometrial samples on day 16 of pregnancy and day 16 of the estrous cycle were analyzed using high-throughput RNA-seq and bioinformatics. The results show that 4666 circRNAs were identified, including 7 downregulated and 11 upregulated differentially expressed circRNAs (DE-circRNAs). Back-splicing and RNase R resistance verified the identified circRNAs. We predicted the competing endogenous RNA (ceRNA) regulatory mechanism and potential target genes of DE-circRNAs. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses of these predicted target genes suggest that DE-circRNAs were significantly involved in establishing endometrial receptivity. Furthermore, Sanger sequencing, qPCR, correlation analysis and Fluorescence in Situ Hybridization (FISH) show that circ_MYRF derived from the host gene myelin regulatory factor (MYRF) might regulate the expression of interferon stimulating gene 15 (ISG15), thereby promoting the formation of endometrial receptivity. These novel findings may contribute to a better understanding of the molecular mechanisms regulating endometrial receptivity and promoting the maternal recognition of pregnancy (MRP).

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Section: Resultssupporting

confidence: 89%

Integrating Analysis to Identify Differential circRNAs Involved in Goat Endometrial Receptivity

Wang

Zang

et al. 2023

IJMS

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“…ISG15 regulates cytokine secretion and immune cell activation through ubiquitination or in its free form [ 31 ]. In domestic animals, ISG15 has been shown to be expressed in epithelial and stromal cells of the endometrium in cattle, horses, and sheep [ 32 , 33 , 34 ] and is located in the nucleus, nuclear stroma, cytoplasm, and organelles of bovine subcellular structures [ 32 ]. In the present study, LPS was used to induce an inflammatory response in gEECs to successfully construct an in vitro model of goat endometritis, as has been previously reported in earlier studies [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

ISGylation Inhibits an LPS-Induced Inflammatory Response via the TLR4/NF-κB Signaling Pathway in Goat Endometrial Epithelial Cells

Xiao

Zhang

et al. 2021

Animals

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Endometritis is a common and important reproductive disease of domestic animals. The principal factors responsible for the disease are infection with Gram-negative bacteria, the release of Lipopolysaccharides (LPS) and activation of the TLR4/NF-κB signaling pathway. However, we do not fully understand the interaction between endometrial immunity and bacterial infection in the disease etiology. The ubiquitin-like protein ISG15 can regulate the TLR4/NF-κB signaling pathway via the ISGylation modification system, modulating the inflammatory response. In the present study, we found that ISG15 protein was expressed mainly in the cytoplasm of goat endometrial epithelial cells (gEECs) and that the expression of key genes and proteins of ISGylation increased in LPS-induced gEECs. Overexpression and silencing of the ISG15 gene demonstrated that ISGylation inhibited an LPS-induced inflammatory response via the TLR4/NF-κB signaling pathway in gEECs. Here, we provide the experimental basis for further exploration of the role of the ISGylation modification system in the inflammatory response of endometrium and a potential method for the treatment of endometritis.

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“…Expression of this gene has been found in parts of the stroma along the uteroplacental interface in gestation. In addition, results from some studies demonstrate that ISG15-conjugated protein levels increase and then decrease during gestation (Alak et al, 2020), which indicates that it is a biologically active molecule that responds to IFN signaling from the conceptus and which temporarily targets proteins for regulation and modification associated with the gestation process ( Jain et al, 2012). Endometrial ISG15 is not simply a consequence of an antiviral state induced by high levels of IFN in the lumen of ruminants at gestational recognition, but is a uterine response to conceptus processes; development, implantation and placentation ( Joyce et al, 2005).…”

Section: Effect Of the Isg15 Gene On Embryonic Development In Sheepmentioning

confidence: 99%

Involvement of the Interferon Tau Gene in Maternal Recognition of Gestation in Sheep

Conde-Hinojosa

Gallegos‐Sánchez

Hernández

et al. 2021

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Objective: To describe the involvement of the interferon tau gene in the maternal recognition of pregnancy in sheep. Design/Methodology/Approach: A search and analysis of the scientific documents retrieved from the Web of Science and Scopus databases related to the functions of the interferon tau gene in the maternal recognition of pregnancy in sheep were conducted. Results: The interferon tau gene (IFNτ) participates in maternal recognition of pregnancy to avoid possible rejection of the embryo, and supports the secretion of progesterone involved in preparing the endometrium for implantation; it also inhibits myometrial motility to maintain pregnancy. IFNτ stimulates the transcription of so-called interferon-stimulated genes (ISGs), which are the effectors of cell-autonomous antiviral defense. One of the representative members of ISGs is the interferon 15-stimulated gene (ISG15) which regulates endometrial receptivity at implantation, as well as survival, growth and development of the conceptus. Study Limitations/Implications: Most embryonic losses occur between fertilization and maternal recognition of pregnancy. Understanding this issue is essential to understanding the possible causes of early pregnancy losses. Findings/Conclusions: Considerable progress has been made in the discovery of how the IFNτ and ISG15 genes act in maternal recognition of gestation in sheep

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Relative abundance and localization of interferon-stimulated gene 15 mRNA transcript in intra- and extra-uterine tissues during the early stages of pregnancy in sheep

Cited by 12 publications

References 25 publications

Integrating Analysis to Identify Differential circRNAs Involved in Goat Endometrial Receptivity

Integrating Analysis to Identify Differential circRNAs Involved in Goat Endometrial Receptivity

ISGylation Inhibits an LPS-Induced Inflammatory Response via the TLR4/NF-κB Signaling Pathway in Goat Endometrial Epithelial Cells

Involvement of the Interferon Tau Gene in Maternal Recognition of Gestation in Sheep

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