Relationships within Eucalyptus (Myrtaceae) using PCR-amplification and southern hybridisation of chloroplast DNA

Abstract

Chloroplast DNA based phylogenetic studies using PCR-amplification and digestion (40 species) combined with Southern blotting (23 species) suggest that Angophora and some representatives of the Eucalyptus subgenera Corymbia and Blakella form a monophyletic group. The subgenera Eudesmia, Monocalyptus, Gaubaea, Idiogenes, Symphyoinyrtus and Telocalyptus appear to form a well defined monophyletic group encompassing most of Eucalyptus, but the exact sister taxon to this group remains unresolved. The results suggest that subgenera Corymbia and Blakella are paraphyletic and that Telocalyptus should be submerged within the subgenus Symnphyoinyrtus.

ITS Sequence Data Resolve Higher Level Relationships Among the Eucalypts

“…The subgenera Corymbia and Blakella have recently been formally separated from the rest of Eucalyptus in a new genus Corymbia (Hill and Johnson, 1995). The most recent view of eucalypt phylogeny, which is relatively consistent across morphological and molecular studies (Hill and Johnson, 1995;Ladiges et al, 1995;Sale et al, 1993Sale et al, , 1996Udovicic et al, 1995) is of two major lineages, one comprising the ''bloodwoods' ' (Angophora and Corymbia) and the other comprising all other subgenera of Eucalyptus.…”

Population genetic analysis and phylogeny reconstruction in Eucalyptus (Myrtaceae) using high-throughput, genome-wide genotyping

“…Because of the expense, a paucity of markers and the large amount of labour involved, only small numbers of samples and markers were used, providing rather coarse resolution of phylogenetic relationships among higher eucalypt taxa (genera and subgenera; Sale et al 1993Sale et al , 1996. As DNA analytical methods progressed and became cheaper, fine-scale restriction site analysis of cpDNA was tested as a means to resolve relationships among closely related species within ser.…”

Phylogeny and classification of Eucalyptus subgenus Eudesmia (Myrtaceae) based on nuclear ribosomal DNA, chloroplast DNA and morphology

“…Outgroup taxa included were E. curtisii Blakely & C.T.White (monotypic subgenus Acerosae), E. tenuipes (subgenus Cuboidea) and E. cloeziana F.Muell. (monotypic subgenus Idiogenes) based on previous eucalypt studies Sale et al 1996;Steane et al 2002). As indicated by their treatment as subgenera by Brooker (2000), there is evidence from morphology and molecular data that these species are outside the main clades of Eucalyptus (subgenera Eudesmia, Eucalyptus and Symphyomyrtus) and therefore they are useful as outgroups.…”

“…Molecular phylogenetic analyses have focussed on the broad eucalypt group and have included different combinations of a few eudesmid species (Sale et al 1993(Sale et al , 1996Ladiges et al 1995;Udovicic et al 1995;Steane et al 1999;Udovicic and Ladiges 2000). Although based on a small sample of eudesmid species, analyses of Sale et al (1993, chloroplast DNA) and Udovicic and Ladiges (2000, chloroplast and nuclear DNA) provided further support for the monophyly of the subgenus, in addition to morphology discussed above.…”

“…Ladiges et al 1995;Udovicic et al 1995;Sale et al 1996;Steane et al 1999;Udovicic and Ladiges 2000). Despite differences of opinion of generic limits, both Hill and Johnson (1998) and Brooker (2000) recognised similar groups, including Eucalyptus subgenus Eudesmia (R.Br.)…”