Relationships of the
 Escherichia coli
 O157, O111, and O55 O-Antigen Gene Clusters with Those of
 Salmonella enterica
 and
 Citrobacter freundii
 , Which Express Identical O Antigens

Samuel, Gabrielle; Hogbin, John-Paul; Wang, Lei; Reeves, Peter R.

doi:10.1128/jb.186.19.6536-6543.2004

Cited by 65 publications

(55 citation statements)

References 29 publications

(29 reference statements)

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“…These included four strains belonging to the O14 and O57 serogroups, for which specific primers were not designed. It is known that Salmonella enterica and Citrobacter freundii share similar but sequence-diversified O-AGCs with E. coli O157, O111, and O55 but produce O antigens that are identical to those produced by E. coli (18). Plainvert et al (19) showed that there are two types of O-AGCs in E. coli O45 strains; one type is related to intestinal pathogenic E. coli such as STEC, and the other is related to extraintestinal pathogenic E. coli.…”

Section: Discussionmentioning

confidence: 99%

Escherichia coli O-Genotyping PCR: a Comprehensive and Practical Platform for Molecular O Serogrouping

et al. 2015

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Each set contained six to nine primer pairs that amplify products of markedly different sizes. This genetic methodology (E. coli O-genotyping PCR) allowed for comprehensive, rapid, and low-cost typing. Validation of the PCR system using O-serogroup references and wild strains showed that the correct O serogroups were specifically and accurately identified for 100% (182/182) and 90.8% (522/575) of references and wild strains, respectively. The PCR-based system reported here might be a promising tool for the subtyping of E. coli strains for epidemiological studies as well as for the surveillance of pathogenic E. coli during outbreaks.

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Section: Discussionmentioning

confidence: 99%

Escherichia coli O-Genotyping PCR: a Comprehensive and Practical Platform for Molecular O Serogrouping

et al. 2015

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“…Three Salmonella O-antigen structures are known to be shared with other enteric organisms: Salmonella serogroup O35 and E. coli O111 have the same antigen structure, as do Salmonella serogroup O50 and E. coli O55, and Salmonella serogroup O30, E. coli O157, and Citrobacter freundii F90. The respective rfb gene clusters from Salmonella, E. coli, and C. freundii with identical O antigens are, in turn, also highly related to one another (50,56).…”

mentioning

confidence: 99%

“…The antigenic differences among the 46 Salmonella O serogroups are due mainly to genetic variation in their respective rfb gene clusters (58). The rfb gene clusters from 15 Salmonella serogroups have been characterized or sequenced: A (32), B (24), C 1 (28), C 2 (5), D 1 (32), D 2 (9), D 3 (9), E (54), O6,14 (18), O17 (19), O18 (19), O35 (56), O30, O50 (50), and O54 (26). The O antigens of serogroups A, B, C 2 , D 1 , D 2 , D 3 , and E have related structures, and their rfb regions are related.…”

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confidence: 99%

Multiplex, Bead-Based Suspension Array for Molecular Determination of Common Salmonella Serogroups

et al. 2007

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We report the development and evaluation of a Salmonella O-group-specific Bio-Plex assay to detect the six most common serogroups in the United States (B, C 1 , C 2 , D, E, and O13) plus serotype Paratyphi A. The assay is based on rfb gene targets directly involved in O-antigen biosynthesis; it can be completed 45 min post-PCR amplification. The assay correctly and specifically identified 362 of 384 (94.3%) isolates tested in comparison to traditional serotyping. Seventeen isolates (4.4%) produced results consistent with what is known about the molecular basis for serotypes but different from the results of traditional serotyping, and five isolates (1.3%) generated false-negative results. Molecular determination of the serogroup for rough isolates was consistent with a common serotype in most instances, indicating that this approach has the potential to provide O-group information for isolates that do not express an O antigen. We also report the sequence of the O-antigenencoding rfb gene cluster from Salmonella enterica serotype Poona (serogroup O13). Compared with other, previously characterized rfb regions, the O13 rfb gene cluster was most closely related to Escherichia coli O127 and O86. The O-group Bio-Plex assay described here provides an easy-to-use, high-throughput system for rapid detection of common Salmonella serogroups.Serotyping of salmonellae is a valuable phenotypic subtyping tool for understanding the epidemiology of this important food-borne pathogen. Salmonella isolates are serotyped using the Kauffmann-White scheme according to their O, H, and Vi antigens (4, 40). The O antigen contains multiple repeats of an oligosaccharide unit (O unit), which, together with lipid A and core oligosaccharides, form the lipopolysaccharide present in the outer membranes of gram-negative bacteria (7). Many of the genes required for O-antigen biosynthesis are organized in a large regulon termed the rfb gene cluster (47). rfb gene clusters have been characterized from a growing number of gram-negative bacteria; this operon is located between galF and gnd in Salmonella enterica and Escherichia coli (49). In general, rfb genes have a low GϩC content (usually less than 40%); the deviation in GϩC content from that of typical S. enterica genes (51%) suggests that rfb DNA originated in species other than S. enterica and was captured by lateral gene transfer (46, 56). Typically, three classes of genes are found in rfb clusters: (i) genes for synthesis of nucleotide sugars specific to the respective O antigen, (ii) sugar transferase genes to build the O subunit, and (iii) the O-antigen polymerase (wzy) and transport protein (wzx) genes for assembly of the O subunit into the O antigen (49).There are 46 O serogroups described in the KauffmannWhite scheme; serogroups were originally designated by alphabetic letters, and then it was necessary to continue with numbers 51 to 67. For consistency in the scheme, all serogroups were given a number designation; however, the most common serogroups (A to E) are commonly designated by ...

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“…There are several pairs of Salmonella and E. coli strains that are known to have identical or closely related O antigen organizations, such as serotype O35 and E. coli O111, serotype O50 and E. coli O55, and serotype O30 and E. coli O157 (33,36). Nonetheless, their gene clusters at the nucleotide level are divergent, and the average identities of wzx and wzy are 70 and 63%, respectively, which is enough difference to discriminate them from each other.…”

Section: Discussionmentioning

confidence: 99%

Development of a DNA Microarray for Molecular Identification of All 46 Salmonella O Serogroups

Guo

Liu

et al. 2013

Appl Environ Microbiol

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e Salmonella is a major cause of food-borne disease in many countries. Serotype determination of Salmonella is important for disease assessment, infection control, and epidemiological surveillance. In this study, a microarray system that targets the O antigen-specific genes was developed for simultaneously detecting and identifying all 46 Salmonella O serogroups. Of these, 40 serogroups can be confidently identified, and the remaining 6, in three pairs (serogroups O67 and B, E1 and E4, and A and D1), need to be further distinguished from each other using PCR methods or conventional serotyping methods. The microarray was shown to be highly specific when evaluated against 293 Salmonella strains, 186 Shigella strains, representative Escherichia coli strains, and 10 strains of other bacterial species. The assay correctly identified 288 (98%) of the Salmonella strains. The detection sensitivity was determined to be 50 ng genomic DNA per sample. By testing simulated samples in a tomato background, 2 to 8 CFU per gram inoculated could be detected after enrichment. This newly developed microarray assay is the first molecular protocol that can be used for the comprehensive detection and identification of all 46 Salmonella O serogroups. Compared to the traditional serogrouping method, the microarray provides a reliable, high-throughput, and sensitive approach that can be used for rapid identification of multiple Salmonella O serogroups simultaneously.

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Relationships of the Escherichia coli O157, O111, and O55 O-Antigen Gene Clusters with Those of Salmonella enterica and Citrobacter freundii , Which Express Identical O Antigens

Cited by 65 publications

References 29 publications

Escherichia coli O-Genotyping PCR: a Comprehensive and Practical Platform for Molecular O Serogrouping

Escherichia coli O-Genotyping PCR: a Comprehensive and Practical Platform for Molecular O Serogrouping

Multiplex, Bead-Based Suspension Array for Molecular Determination of Common Salmonella Serogroups

Development of a DNA Microarray for Molecular Identification of All 46 Salmonella O Serogroups

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