Relationships of microclimatic variables to colonization of rose debris byBotrytis cinereaand the biocontrol agentClonostachys rosea

Abstract

Biological control of Botrytis cinerea by Clonostachys rosea is an alternative to chemical control of rose Botrytis blight in greenhouses. Environmental conditions affect the colonization of senescing and dead tissues by both fungi. The contribution of microclimatic variables to debris colonization/sporulation by both fungi was estimated by path coefficient analysis. We monitored daily values of: maximum, average, and minimum temperatures (T max , T ave , and T min ), and relative humidity (RH max , RH ave , and RH min ); accumulated rainfall; vapour pressure deficit average; hours with RH /90% (RH 90 ); and average temperature during RH 90 (T ave90 ). Association of variables accumulated between the first and seventh day before sampling explained colonization/sporulation variation: R 2 0/0.81 Á0.86 for B. cinerea and 0.91 Á0.96 for C. rosea . RH max and RH 90 were the main factors that directly favoured colonization/sporulation of both fungi. Colonization/sporulation negatively correlated with RH min , T min , and T ave for B. cinerea and T min , T ave , and T ave90 for C. rosea . The antagonist can suppress B. cinerea colonization/sporulation on rose debris under a wide range of environmental conditions.

In vitro evaluation of drying supports and adhesive polymers as adjuvants for biocontrol of Diplodia seriata by Trichoderma harzianum and Clonostachys rosea

“…(Jensen et al, 2004), Sclerotinia sclerotiorum (Lib.) de Bary (Rodríguez et al, 2011), and others (Jensen et al, 2000;Morandi et al, 2003;Morandi et al, 2007). Trichoderma harzianum Rifai and C rosea have also been tested individually and together, as conidium suspensions, for the control of Diplodia seriata and Neofusiccocum australe in greenhouse and field assays (Arriagada, 2015).…”

Brazilian isolates of Clonostachys rosea: colonization under different temperature and moisture conditions and temporal dynamics on strawberry leaves

“…2000) even under conditions favourable for the pathogen (Morandi et al. 2003), while the ecological requirements for both pathogen and antagonist were similar (Morandi et al. 2006).…”

“…This isolate suppressed B. cinerea sporulation and controlled grey mould in several hosts (Peng and Sutton 1991;Sutton et al 1997Sutton et al , 2002Morandi et al 2003). Regardless of developmental stages of rose tissues, Cr established and suppressed B. cinerea sporulation (Morandi et al 2000) even under conditions favourable for the pathogen (Morandi et al 2003), while the ecological requirements for both pathogen and antagonist were similar (Morandi et al 2006).…”