Relationships between some transcription factors and concordantly expressed drought stress-related genes in bread wheat

Expression Profiling of Salt-Responsive Genes and Transcription Factors in Leaf Transcriptome of Arabidopsis thaliana

“…Cluster 64 included the TF gene NFYC3 encoding nuclear transcription factor Y C-3 subunit and the salt-responsive gene RFS6 encoding galactinol-sucrose galactosyltransferase 6. In light of our prior research on the COL1 gene encoding zinc finger CONSTANS transcription factors under drought stress in bread wheat (Triticum aestivum), which demonstrated a relationship via qPCR between the COL1 gene and the stress-responsive gene TSA encoding tryptophan synthase [17], the present study has corroborated this finding (Figure 4). In the current investigation, two versions of the COL gene, namely COL2 in cluster 30 and COL8 in cluster 11, exhibited analogous expression patterns, characterized by upregulation at the 12 h time point of salt (200 mM NaCl) stress, akin to the salt-responsive gene TSA within cluster 4 in A. thaliana leaves (Figure 4 and Tables S2, S3 and S7).…”

“…To validate the concordant expression of selected TFs and salt-responsive genes under salt stress conditions in tobacco VIGS lines, the expression levels of 4-week-old tobacco VIGS lines were quantified through quantitative Polymerase Chain Reaction (qPCR), as outlined in the extant literature [17]. A comprehensive roster of the six concordantly expressed TFs and salt-responsive genes, germane to the validation experiment, is presented in Table S1, with actin and ubiquitin genes serving as dual housekeeping genes throughout the qPCR validation process.…”

“…A precedent in this avenue of exploration reveals the coexistence of TFs and heat stress-responsive genes in conferring augmented stress tolerance, notably in the contexts of heat and water deficit stresses [16]. Further strides were undertaken in the investigation of concordant TF and stress-responsive gene expression via RNA-Seq modalities, focusing on botanical exemplars such as the love apple (Paris polyphylla) and wheat (Triticum aestivum), unveiling salient associations through the prism of knockout methodologies [17,18]. Against this backdrop, the motivation behind this investigation, aiming to harness RNA-Seq datasets of Arabidopsis thaliana under salt stress to unearth TFs characterized by their concordant expression alongside salt stress-responsive genes.…”