Relationships between seminal plasma arginase activity and spermatological parameters in rams

Abstract

This study was conducted to evaluate the correlation between seminal plasma arginase activity and spermatological parameters in rams. In this study, five fertility-proven Awassi rams were used as material. Six ejaculates were collected from each ram by an artificial vagina. Spermatological parameters (semen volume, mass activity, sperm motility and concentration and abnormal sperm rate) were immediately determined in each ejaculate. For enzyme assay, the semen samples were centrifuged and stored at -20 °C for the analysis of arginase activity. The average seminal plasma arginase activity was 0.61 ± 0.20 U (mg protein)(-1) . There was a positive correlation between arginase activity and semen volume (r = 0.412, P< 0.05), semen mass activity (r = 0.610, P < 0.01), sperm motility (r = 0.447, P < 0.05) and sperm concentration (r = 0.808, P < 0.01). However, there was a negative correlation between arginase activity and abnormal sperm rate (r = -0.424, P < 0.05). In conclusion, this study clearly suggests that there is a significant correlation between seminal plasma arginase activity and spermatological parameters. In light of these results, seminal plasma arginase activity may be a biochemical criterion for determining sperm quality besides classical semen analysis parameters in rams.

Influence of vitamin E and vitamin E‐selenium combination on arginase activity, nitric oxide level and some spermatological properties in ram semen

“…However, there was an increase in seminal plasma arginase activity in vitamin E + selenium injected group, except for 72nd hr, but this increase was not statistically significant. Although some relationships between spermatological parameters and seminal plasma arginase activity have been reported (Gür & Kandemir, 2012;Türk, Gür, Kandemir, & Sönmez, 2011), no evidence was found effect of vitamin E and/or vitamin E + selenium combination on seminal plasma arginase activity.…”

“…However, there was an increase in seminal plasma arginase activity in vitamin E + selenium injected group, except for 72nd hr, but this increase was not statistically significant. Although some relationships between spermatological parameters and seminal plasma arginase activity have been reported (Gür & Kandemir, ; Türk, Gür, Kandemir, & Sönmez, ), no evidence was found effect of vitamin E and/or vitamin E + selenium combination on seminal plasma arginase activity. However, in a study conducted by adding vitamin E and selenium to diet, a significant increase in cardiac and renal arginase activity and a decrease hepatic arginase activity was observed in vitamin E ingested group, but no difference was found in the vitamin E + selenium combination group compared to the control group (Erişir, Beytut, Ozan, & Aksakal, ).…”

The kinetic properties of arginase in sperm cells of inferile men

“…It was found that plasma arginase activity was significantly lower than in the non-stress situation and during stress there was a negative correlation between the percentage of rapid progressive motility and arginase activity (Eskiocak et al, 2006). Similar relationships between the plasma arginase activity and the sperm concentration and sperm motility were detected in sheep and bulls (Gür et al, 2012;Türk et al, 2011). Although arginase activity has usually been detected in the reproductive tract, including spermatozoa, no data relating to the kinetic properties of enzyme in ejaculated spermatozoa has been reported.…”

“…Arginase activity was detected not only in spermatozoa, bu also in seminal plasma. A positive correlation has been shown between arginase activity and semen volume, semen mass activity, sperm motility and sperm concentration (Gür et al, 2012). It was found that plasma arginase activity was significantly lower than in the non-stress situation and during stress there was a negative correlation between the percentage of rapid progressive motility and arginase activity (Eskiocak et al, 2006).…”

The Peculiarities of Arginase Pathway of L-Arginine in Spermatozoa in Men With Different Forms of Pathospermia

“…Встановлено наявність позитивного кореляційного зв'язку між активністю аргінази і концентрацією сперматозоїдів, активністю аргінази й індексом рухливості сперматозоїдів. Аналогічні залежності між активністю аргінази сім'яної плазми та концентрацією і рухливістю сперматозоїдів виявлено у баранів і биків [15,16]. Слід зауважити, що отримані нами результати суперечать даним дослідників, які виявили, що аргіназна активність сперматозоїдів неплідних чоловіків з олігозооспермією є суттєво вищою, ніж у здорових донорів [13].…”