Relationships between molecular weight and fluorescence properties for size-fractionated dissolved organic matter from fresh and aged sources

Cuss, Chad W.; Guéguen, Céline

doi:10.1016/j.watres.2014.10.013

Cited by 99 publications

(67 citation statements)

References 68 publications

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“…Studies in recent years have demonstrated the benets of DOM multidetector analysis to the interpretation of uorescence. [24][25][26] This approach combines the strengths of individual detection methods and thus may provide further advances in the characterization of DOM.…”

Section: Discussionmentioning

confidence: 99%

Emerging patterns in the global distribution of dissolved organic matter fluorescence

et al. 2019

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The spectra responsible for natural dissolved organic matter (DOM) fluorescence in 90 peer-reviewed studies have been compared using new similarity metrics. Numerous spectra cluster in specific wavelength regions. The emerging patterns suggest that most fluorescence spectra are not tied to biogeochemical origin, but exist across a wide range of different environments. † Electronic supplementary information (ESI) available: Denitions of shi-and shape sensitive congruence , information on the processing of OpenFluor models, and meta-analysis of model similarities. See

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Section: Discussionmentioning

confidence: 99%

Emerging patterns in the global distribution of dissolved organic matter fluorescence

et al. 2019

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“…Several studies have utilized HPSEC and demonstrated how this technique can be valuable for characterizing the composition of DOM. For example, using techniques similar to HPSEC for a set of freshwater samples and leaf leachates, Cuss and Guéguen () have suggested that, contrary to the size‐reactivity continuum for marine DOM, DOM increases in apparent molecular size with aging while protein‐like fluorophores seem to get encapsulated into lager moieties. Similarly, fluorescence and absorbance spectroscopy are a cost‐efficient techniques to trace dynamics and turnover of DOM and have thus been used extensively in the past decades (e.g., Coble ; Kothawala et al ; Gonçalves‐Araujo et al ).…”

Section: Discussionmentioning

confidence: 99%

Unraveling the size‐dependent optical properties of dissolved organic matter

Wünsch

Stedmon

Tranvik

et al. 2017

Limnology & Oceanography

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The size‐dependent optical properties of dissolved organic matter (DOM) from four Swedish lakes were investigated using High Performance Size Exclusion Chromatography (HPSEC) in conjunction with online characterization of absorbance (240–600 nm) and fluorescence (excitation: 275 nm, emission: 300–600 nm). The molecular size of chromophoric DOM (CDOM) was consistently higher than that of fluorescent DOM (FDOM), with an average difference of 0.37 kDa. The relative abundance of FDOM vs. CDOM ranged from 0.3 to 0.7 across lakes, and increased with decreasing average molecular size. Across sites, the CDOM spectral slopes of the large molecular size fraction were highly similar while the low molecular size fraction differed and contributed to different bulk spectral slopes. Our results indicate structural congruence of high molecular size DOM across systems while lake trophic status determined the characteristics of the low size range. Furthermore, the combination of HPSEC and parallel factor analysis (HPSEC‐PARAFAC2) allowed the decomposition of DOM fluorescence chromatograms. Three humic‐like components and one protein‐like component with broadly overlapping molecular size distributions were identified. This overlap provides further evidence for the supramolecular assembly hypothesis since fluorophores, as revealed by PARAFAC2, occur in aggregates of overlapping molecular size. Our results further suggest a link between the molecular size of these fluorophores and the associated supramolecular assemblies. This study demonstrates the potential for HPSEC and novel mathematical approaches to provide unprecedented insights into the relationship between optical and chemical properties of DOM in aquatic systems.

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“…We used three-dimensional Excitation Emission Matrices (EEMs) derived from total fluorescence scans to broadly characterize dissolved organic matter (DOM) into humic-like and protein-like fractions and to correlate specific fluorophores with those previously identified in the literature. DOM fluorescence was measured in ratio mode (S/R) using an Agilent G1321B fluorescence detector (Agilent Technologies, Santa Clara, USA) and methods outlined by Cuss and Gueguen (2015). Prior to each analysis, the system was rinsed 3 times with deionized water and 3 times with sample at room temperature.…”

Section: Dom Characteristicsmentioning

confidence: 99%

Basal thermal regime affects the biogeochemistry of subglacial systems

Dubnick¹,

Sharp²,

Danielson³

et al. 2019

Preprint

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Abstract. Ice formed in the subglacial environment can contain some of the highest concentrations of solutes, nutrients, and microbes found in glacier systems. Upon glacial melt, these materials are released to downstream freshwater and marine ecosystems and glacier forefields. Despite the potential ecological importance of basal ice, our understanding of its biogeochemical characteristics, and their spatial and temporal variability, remains limited. We hypothesize that the basal thermal regime of glaciers is a dominant control on subglacial biogeochemistry because it influences the degree to which glaciers mobilize material from the underlying substrate and controls the nature and extent of biogeochemical activity that occurs at glacier beds. Here, we characterize the solutes, nutrients, and microbes found in the basal regions of a cold-based glacier and three polythermal glaciers and compare them to those found in overlying glacier ice. Compared to its parent glacier ice, basal ice from polythermal glaciers was consistently enriched in major ions, dissolved organic matter (including a specific fraction of humic-like fluorescent material), and microbes, and occasionally enriched in dissolved phosphorus and reduced nitrogen (NH4+) and in a second dissolved component of humic-like fluorescent material. In contrast, the biogeochemistry of basal ice from the cold-based glacier was remarkably similar to that of its parent glacier ice. Although basal ice from the cold-based glacier may have acquired some inorganic and organic nutrients from the underlying substrate, it did not appear to contain significant amounts of either solutes or microbes derived from the glacier bed. These findings suggest that a glacier's basal thermal regime can play an important role in determining the mix of solutes, nutrients, and microbes that are acquired from subglacial substrates and/or produced in situ.

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Relationships between molecular weight and fluorescence properties for size-fractionated dissolved organic matter from fresh and aged sources

Cited by 99 publications

References 68 publications

Emerging patterns in the global distribution of dissolved organic matter fluorescence

Emerging patterns in the global distribution of dissolved organic matter fluorescence

Unraveling the size‐dependent optical properties of dissolved organic matter

Basal thermal regime affects the biogeochemistry of subglacial systems

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