Relationships Between Barley Hordeins and Malting Quality in a Mutant of cv. Triumph. II. Genetic and Environmental Effects on Water Uptake

Molina-Cano, José Luis; Sopena, A.; Polo, J.P.; Bergareche, Carmen; Moralejo, Marian; Swanston, J. S.; Glidewell, S. M.

doi:10.1006/jcrs.2002.0440

Cited by 42 publications

(39 citation statements)

References 25 publications

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“…Furthermore, only few studies have evaluated protein composition in mature barley grains and relations to malt quality (Molina-Cano et al, 2002;Wang et al, 2007). Studies about changes in protein composition during malting and how this is related to protein composition in mature grainare even scarcer.…”

Section: Introductionmentioning

confidence: 99%

Soil and starter fertilizer and its effect on yield and protein composition of malting barley

Malik

Holm

Johansson

2012

J. Soil Sci. Plant Nutr.

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Fertilizer application and growing locations are known to influence yield and protein concentration of malting barley. The aim of the present investigation was to evaluate the influence of soil and starter fertilizer on yield and protein composition in mature and maltedbarley. The cultivar Prestige was grown in two different soils (Lunnarp and LaxmansÅkarp) in combination with the use/non-use of starter fertilizer in climate chambers. Yield parameters, protein concentration and composition was measured. Effect of soil on plant emergence, yield and protein composition was significant while the effect of starter fertilizer was not. More nitrogen rich and low humus content soil (Lunnarp) resulted in higher grain yield and polymerization of proteins and lower protein concentration than the other soil. Combination of soil and starter fertilizer influenced protein composition in mature and malted barley. Breakdown of proteins were significantly higher at certain combination of soil and starter fertilizer than with other combinations. The Lunnarp soil combined with starter fertilizer was preferable to obtain high yield, low protein concentration and large grain size in mature grains. When breakdown of proteins at malting was taken into consideration as well, Lunnarp soil together with no starter fertilizer might be the best option.

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Section: Introductionmentioning

confidence: 99%

Soil and starter fertilizer and its effect on yield and protein composition of malting barley

Malik

Holm

Johansson

2012

J. Soil Sci. Plant Nutr.

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“…The quantity and distribution of the B-hordeins were shown to be important factors for malting quality. The high B-hordein content in the sub-aleurone region (Molina-Cano et al 2002) and low D/B hordein ratio were well associated with improved malting quality (Peltonen et al 1994). Allelic variation of the B-hordein genes is therefore important for genetic improvement of barley for malt quality because specific alleles of the B-hordein gene family can be enriched without affecting their biological properties .…”

Section: Introductionmentioning

confidence: 99%

“…Prolamins in barley (also called hordeins), the major storage proteins in the endosperm, are one of the major factors affecting food processing, malting and feed quality of barley (Peltonen et al 1994;Liu et al 1996;Molina-Cano et al 2002). Prolamins contain polymeric components with interchain disulfide bonds and monomeric components with intrachain disulfide bonds.…”

Section: Introductionmentioning

confidence: 99%

Structural and expressional analysis of the B-hordein genes in Tibetan hull-less barley

Han

Deng

et al. 2009

Genetica

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The B-hordein gene family was analyzed from two Tibetan hull-less barley cultivars Z09 and Z26 (Hordeum vulgare subsp. vulgare). Fourteen B-hordein genes, designated BZ09-2 to BZ09-5 (from Z09) and BZ26-1 to BZ26-10 (from Z26), were sequenced. Seven of them, similar to a previously reported BZ09-1 from Z09, were predicted to encode putative active proteins each with a signal peptide, a repetitive domain, and a C-terminal region; seven of them were predicted to be pseudogenes. The B-hordein gene family was analyzed using all known representatives of B-hordein sequences and two most similar LMW-GSs of Triticum aestivum. Alignment of these seven putative proteins with known B-hordeins and two most similar LMW-GSs of T. aestivum revealed that they shared a common motif. A large variation was observed between numbers of repeat units of predicted B-hordeins of Z26 and Z09. Phylogenetic analysis revealed that all BZ26 clones were clustered in a subgroup, and BZ09-1 formed another subgroup by itself in the putative eight active genes. In addition, six 5 0 -upstream regulatory sequences of the B-hordein genes were isolated from the two accessions by a single oligonucleotide nested PCR, and several different mutations were identified in the cis-acting element GLM and two distinctive sequences (Z09P-2 and Z26P-3). Phylogenetic analysis of 5 0 -upstream regulatory regions of the B-hordein genes showed that members from the same accession were clustered together except for two distinct members. Quantitative real time PCR analysis indicated distinct expression levels of B-hordein genes in four developing stages of developing grains in two accessions. These findings suggest B-hordein genes have intrinsic differences between accessions, and this knowledge will be useful for incorporating the B-hordeins protein in barley breeding programs.

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“…Moreover, no obvious C 259 hordeins profiles were found in Dhow. Previous studies showed 260 that a high total content of C hordein was consistently associated 261 with greater water absorption during malting and better malting 262 quality [25]. Recently, Holopainen et al [14] proved that C hordeins (Fig.…”

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confidence: 99%

“…Hordein (prolamin) and 20 glutelin are the two major storage proteins accounting for 35-55% 21 and 35-40% total protein, respectively, and are mainly contained in 22 barley endosperm. Based on their electrophoresis mobilities and 23 sulphur content, hordeins are classified into three broad groups 24 named sulphur-rich (S-rich), sulphur-poor (S-poor) and high 25 molecular weight (HMW) prolamins, with several subgroups 26 within the S-rich group [35]. The B (S-rich, 28-45 kDa) and 27 C (S-poor, 49-72 kDa) hordeins represent the two main fractions 28 accounting for 70-90% and 10-20% of the total hordeins, 29 respectively, while the D (around 100 kDa) and g fractions 30 (molecular mass less than 20 kDa) are the minor components 31 (less than 5% of the total hordein fraction) [34].…”

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confidence: 99%

MALDI-TOF mass spectrometry provides an efficient approach to monitoring protein modification in the malting process

Dai

Harasymow

Roumeliotis

et al. 2014

International Journal of Mass Spectrometry

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Proteins in barley grains are determinants of beer quality, which are modified during malting to provide nutrition for yeast during brewing. Different malting barley varieties behave differently during malting. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to characterize storage proteins including albumin, globulin, hordein and glutelin in grains and malt of parental varieties and a doubled haploid (DH) population. Each parental variety produces malt with particular quality profiles. The protein profiles of analyzed extracts in seed and malt were compared to find the most influenced proteins during the malting process. Our results revealed that malting procedure dramatically affected the composition and amounts of albumin, then hordein, glutelin and globulin in barley. Moreover, hordein and glutelin profiles in the DH population exhibited high levels of polymorphism. The genetic information represented by individual hordein and glutelin profiles was successfully mapped as molecular markers in a genetic linkage map. Twelve and 8 (hordein in seed and malt, respectively) or 10 and 8 (glutelin in seed and malt, respectively) segregating peaks were scored as polymorphic across the population, and all peaks were located to the chromosome 1H. The MALDI-TOF MS provides a method with high resolution and throughput to characterize mass patterns of extracted storage proteins in barley and malt.

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Relationships Between Barley Hordeins and Malting Quality in a Mutant of cv. Triumph. II. Genetic and Environmental Effects on Water Uptake

Cited by 42 publications

References 25 publications

Soil and starter fertilizer and its effect on yield and protein composition of malting barley

Soil and starter fertilizer and its effect on yield and protein composition of malting barley

Structural and expressional analysis of the B-hordein genes in Tibetan hull-less barley

MALDI-TOF mass spectrometry provides an efficient approach to monitoring protein modification in the malting process

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