Abstract:In an effort to improve upon tomato varieties on the Ghanaian market, this study was carried out to identify genotypes with desirable qualities for further breeding work. The study was executed by crossing some varieties of Solanum lycopersicon; cherry red, cherry yellow, roma and wosowoso with a wild tomato, Solanum pimpinellifolium. The progenies were cultivated for four generations to obtain these lines. Fruits harvested from these lines of F 4 were analysed for the following physico-chemical properties; dry matter, pH, total titratable acidity, total soluble solids and vitamin C. The total soluble solids and total titratable acidity were used to deduce flavour and sweetness indices for the fruits. Significant (p<0.05) differences were observed among and within the various breeding lines. The dry matter, pH and total soluble solids of the fruits ranged from 5.21-8.43%, 4.08-4.59 and 4.00-7.80% respectively. Total titratable acidity, ascorbic acid, flavour and sweetness indices also ranged from 0.35-0.88%, 16.84-46.09 mg/100g, 0.07-0.16 and 5.33-13.64 respectively. In all wosowoso breeding lines were the most promising of all the breeding lines. Most of the progenies obtained had characteristics significantly (p<0.05) different from each other and then from those of their respective parents (controls). This shows that the various lines were still in the process of segregating and had not yet attained the status of pure lines.