Relationships among the cervical mucus urea and acetone, accuracy of insemination timing, and sperm survival in Holstein cows

Beran, Jan; Stádník, Luděk; Ducháček, Jaromír; Okrouhlá, Monika; Doležalová, Martina; Kadlecová, Veronika; Ptáček, Martin

doi:10.1016/j.anireprosci.2013.09.005

Cited by 17 publications

(19 citation statements)

References 28 publications

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“…The values of spermatozoa motility during TDT declined; this finding is in accordance with the results of previous studies (Defoin et al 2008;Beran et al 2011). The results reported by Stadnik et al (2002) and Beran et al (2013a) indicated individual differences in resistance of sperm to freezing as well as to environmental conditions after thawing, theoretically including the conditions in cow vagina and uterus after insemination. From this point of view, results of TDT should be very interesting and important.…”

Section: Discussionmentioning

confidence: 90%

“…Growing interest in improving the quality of frozen bull semen for the needs of the market in recent years has been determined by the declined fertility of dairy cows (Beran et al 2013a, Stadnik et al 2015a) and world-wide practical usage of artificial insemination (Shahverdi et al 2014). Therefore, it is necessary to ensure optimal genetic sires selection (Zhang et al 2015) as well as semen processing including semen collection (Dzyuba et al 2015), dilution (Meamar et al 2016), slow cooling to 4-6°C (Kristan et al 2014), and equilibration (Beran et al 2013b) as well as cryopreservation maintaining spermatozoa fertilization capability (Andrabi 2007) evaluated objectively if possible (Simonik et al 2015).…”

Section: Introductionmentioning

confidence: 99%

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Equilibration and freezing interactions affecting bull sperm characteristics after thawing

Doležalová¹,

Stádník²,

Biniová³

et al. 2016

CZECH J ANIM SCI

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ABSTRACT:The objective was to determine effects of equilibration length and freezing curve type as well as their interactions on motility and live spermatozoa proportion in bull sperm after thawing. The ejaculates of 6 sires were repeatedly collected. Fresh semen was diluted with one extender and divided into 3 groups equilibrated for 30, 120, and 240 min. Subsequently, half straws of each group were frozen using standard 3-phase or 2-phase freezing curve differing in the rate of temperature decrease. Th e spermatozoa motility (M) was evaluated immediately after thawing and at 30, 60, 90, and 120 min of thermodynamic test (TDT). Live spermatozoa proportion was evaluated after thawing and at the end of TDT. Average of spermatozoa motility (AM), decrease of spermatozoa motility (MD), average proportion of live spermatozoa (ALS), and decrease of live spermatozoa proportion (DLS) through the TDT were calculated. Significant inter-sire differences in AM (0.45-17.0%; P < 0.05-0.01), MD (0.76-12.57%; P < 0.05-0.01), and ALS (0.99-23.8%; P < 0.01) were detected. The longest equilibration ensured the highest M during TDT and AM (+2.72 and +4.58%; P < 0.05-0.01), however higher MD (+4.06%; P < 0.01) compared to standard length as well. Straws freezed using 2-phase curve achieved higher M through TDT, AM (+7.3%; P < 0.01) as well as ALS (+11.77%; P < 0.01). The 2-phase curve presented higher M compared to the 3-phase freezing curve within all equilibration lengths. Significant differences in AM, MD,; P < 0.05-0.01) between equilibration length vs freezing curve interactions were determined. Results document the importance of equilibration length, freezing curve, and their interaction effect on live spermatozoa proportion and sperm motility after thawing as well as necessity of individual conditions for bulls semen processing and insemination doses production.

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Section: Discussionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Equilibration and freezing interactions affecting bull sperm characteristics after thawing

Doležalová¹,

Stádník²,

Biniová³

et al. 2016

CZECH J ANIM SCI

Self Cite

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“…And in addition, decreases ferny-like patterns and increases atypical, and/or stellate structures. In general, it causes reduction in CM quality 4 . In the estrus, under the influence of estrogen, there is an increase in muco-protein secretions, sodium chloride and water content of CM which alter the pH of the CM before, during and after estus, Alkaline pH of CM is favorable for sperm motility 43 , so, with increased levels of estrogen, probably CM quality improves.…”

Section: Discussionmentioning

confidence: 99%

Effects of eCG in Milking Dairy Cows with High Levels of BUN and Synchronized with Ovsynch Protocol

Karami¹,

Rad²,

Mesgaran³

et al. 2016

Biosci., Biotech. Res. Asia

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Previous research indicates that high blood urea nitrogen (BUN) concentrationsare associated with decreased fertility in lactating dairy cows.This experiment was done to evaluate the effects of equine chorionic gonadotropin (eCG) administration (with or without eCG) on fertility of lactating dairy cows with different levels of BUN subjected to fixed-time artificial insemination (FTAI).Ovulation was synchronized in all cows using Presynch-Ovsynch program and start 37 days post parturition(pp). Blood samples were collected on days 50-55 pp to assess BUN and cows were classified as with high urea when BUN was 20< ng/mL(n=64); otherwise, they were classified as cows with optimal urea when BUN was >10 and <15 (n=62). Within each group, cows were assigned randomly to receive either 600 IU eCG concurrent with PGF2a treatment of the Ovsynch protocol (treatment cows)or with no further treatment (control cows). Blood samples were collected on Days 0 and 5 after FTAI to evaluate the effect of eCG administration on synchornization and plasma concentration of P4. Pregnancy diagnoses were performed by ultrasound. No treatment effects were detected for Pregnancy per AI at Days 30. However, P/AI at Days 30 was not affected by treatment with eCG but P/AI at 60 days post-AI in cow with high BUN levels in control group was less and differed significantly than treatment group with optimal BUN (OR=4.722; P=0.016). This differ significant, P/AI at 60 days post-AI no observed in cow with high BUN that treatment with eCG. P4 concentrations on Days 5 differed significantly between treatments and control group in both levels of BUN concentration. Inclusion of eCG in ovsynch protocol increased progestron concentrations in early time of luteal phase after AI. The use of eCG in ovsynch protocol will improve reproductive efficiency in dairy cows with high levels of BUN.

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“…Cattle fertility has been intensively studied in recent years with regard to females (Hanuš et al 2010;Beran et al 2013a). However, cows' reproduction results are affected by the male component as well.…”

Section: Reproduction Bull Sperm Extender Ldl Cholesterol Sperm Smentioning

confidence: 99%

Influence of selected factors on bovine spermatozoa cold shock resistance

et al. 2015

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The objectives of this study were to determine the effects of sire, extender, and addition of Low Density Lipoprotein (LDL) to extenders used on the percentage rate of spermatozoa survival after cold shock. Two groups of extenders were compared: without LDL addition (control variants) and LDL enriched (experimental variants). Three extenders were used: AndroMed ® extenders, and 6-10% LDL addition into the Triladyl ® extender. In total, 12 samples of fresh semen were collected from 4 bulls during a period of 8 weeks. Bovine spermatozoa cold shock resistance (1 ± 1 °C, 10 min) was evaluated by the percentage rate of live sperm using eosin-nigrosine staining immediately and after heat incubation (37 ± 1 °C, 120 min). The results showed the effect of sire as important and individual differences between selected sires in their sperm resistance against cold shock were confirmed. AndroMed ® and Bioxcell ® were found to be providing better protection of bull semen to cold shock compared to Triladyl ® due to lower decline of live sperm proportion. Our results detected a positive effect of LDL addition on sperm resistance against cold shock, especially on lower decrease of live sperm percentage rate after 120 min of the heat test (P < 0.05). Further studies are needed to assess the optimal concentration of LDL in various kinds of extenders as well to state ideal time and temperature conditions for ensuring LDL reaction with sperm. , bull sperm, extender, LDL cholesterol, sperm survival, cold shock, eosin-nigrosine staining Reproduction

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Relationships among the cervical mucus urea and acetone, accuracy of insemination timing, and sperm survival in Holstein cows

Cited by 17 publications

References 28 publications

Equilibration and freezing interactions affecting bull sperm characteristics after thawing

Equilibration and freezing interactions affecting bull sperm characteristics after thawing

Effects of eCG in Milking Dairy Cows with High Levels of BUN and Synchronized with Ovsynch Protocol

Influence of selected factors on bovine spermatozoa cold shock resistance

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