Relationships among genera in Saniculoideae and selected Apioideae (Umbelliferae) inferred from nrITS sequences

Abstract

The internal transcribed spacers (ITS1 and ITS2) of 18S-26S nuclear ribosomal DNA were newly sequenced for eight species of Umbelliferae (six species from subfamily Saniculoideae: Actinolema macrolema, Astrantia minor, Eryngium giganteum, E. coeruleum, Hacquetia epipactis, and Lagoecia cuminoides, two species from subfamily Hydrocotyloideae: Dickinsia hydrocotyloides and Azorella trifurcata), as well as Hohenackeria exscapa, a species of uncertain position in the family. Phylogenetic analyses of new data, plus previously reported sequences of 52 other species using neighbor-joining, maximum parsimony, and maxim um likelihood methods yielded similar results: (1) Actinolema is sister to Astrantia corresponding to Drude's treatments; (2) in Astrantia, molecular divergence is revealed between sects. Astrantiella (A. minor) and Astrantia (A. major, A. maxima); (3) Eryngium appears to be paraphyletic; (4) Hacquetia might be treated as a part of Sanicula; and (5) Lagoecia is very distant from all other Saniculoideae and close to some genera of Apioideae. Our results correspond to matK data previously published: (1) Hohenackeria forms a clade with Bupleurum, in a position near the base of the Apioideae tree; (2) Azorella is sister to a large cluster uniting all Saniculoideae and Apioideae, being slightly closer to them than to the Hydrocotyle-Araliaceae clade; (3) Dickinsia is very distant from phenetically similar Hydrocotyle, falling within a large cluster of Apioideae, but also including Lagoecia and Naufraga.

Integrated Molecular and Morphological Studies of the <I>Daucus guttatus</I> Complex (Apiaceae)

“…Sáenz (1981), Pujadas Salvà (2003, Baranski et al (2006), and Mezghani et al (2014) indicated that fruits provided critical taxonomic characters distinguishing taxa within Daucus since fruits tend to show less phenotypic plasticity in comparison with other plant organs. Other studies questioned the utility of fruit characters alone as a practical tool to classify taxa in the Apiales (Downie and KatzDownie 1999;Katz-Downie et al 1999;Spalik et al 2001;Vallejo-Roman et al 2002;Lowry et al 2004). We investigated this issue in the present study by examining only the 10 continuously variable fruit characters.…”

Composition of the essential oil of Petagnaea gussonei (Sprengel) Rauschert, a relict species from Sicily (Southern Italy)

“…Besides, the diagnostic components of P. gussonei (such as germacrene D) are absent in Lagoecia. Considering the data on chemical composition of the essential oil as well as the molecular and fruit-anatomy characteristics, [33][34][35] the taxonomy of the tribe Lagoecieae might be considered to be reviewed. The tribe of Lagoecieae might be judged as very arti¼cial, in fact Lagoecia is very distant from P. gussonei and the other taxa belonging to the Saniculoideae.…”

Are Lotus creticus and Lotus cytisoides (Leguminosae) closely related species? Evidence from nuclear ribosomal ITS sequence data

“…Total DNA was isolated from leaf tissue using NucleoSpin Plant isolation kit (Macherey-Nagel, Düren, Germany) following the manufacturer's instructions. Details of PCR amplifications of ITS1-2 region and sequencing strategies used (including primer locations and characteristics) are provided in Valiejo-Roman et al (2002). PCR products were purified using the DNA cleaning kit from Cytokine (St.-Petersburg, Russia) following the manufacturer's instructions.…”