Random isonymy and inbreeding in a population may be affected by demographic variation, particularly population size and migration. Total levels of isonymy and inbreeding are also affected by cultural behaviors, which may themselves be affected by demographic factors. This paper investigates the relationship of demographic variation and inbreeding components (total, random, nonrandom) in five towns in north-central historical Massachusetts, 1741-1849. Estimates of exogamy rates (the proportion of individuals who are migrants) were derived from 6,038 marriage records, and population sizes were obtained from census data. Inbreeding components were computed using the surnames of 5,791 marriages. A total of 72 marriages are isonymous, giving a n estimated total inbreeding coefficient of 0.0031. Demographic and inbreeding measures were computed for 33 samples defined on the basis of town and marriage cohort. The interrelationship between demographic variation and inbreeding was tested using rank order correlation over all 33 samples. Total inbreeding within samples shows no significant correlation with any of the demographic variables. Random inbreeding within samples shows a significant negative correlation with population size, and nonrandom inbreeding shows a positive correlation with population size. Various explanations for these correlations are offered. The opposite influences on random and nonrandom inbreeding cancel one another. These results demonstrate the complex interaction between cultural factors, demographic variation, and genetic structure in human populations.The study of inbreeding in human populations provides a unique focus for the analysis of interactions between cultural and biological processes. In theory, the level of inbreeding in a population depends on population size, migration rates, and the number of generations since the population was founded (Crow and Kimura, 1970). Inbreeding in human populations may also be influenced by cultural patterns of mate choice, demographic constraints, and geographic dispersal, among other factors. At the level of the local population, the cultural, demographic, and genetic aspects of inbreeding may be interrelated, making interpretation difficult.Inbreeding may be ascertained through analysis of church dispensations, pedigrees, or surnames. Inbreeding is relatively easy to estimate from isonymy (shared surnames) in certain populations, particularly in Western Europe and North America, where records are more abundant than in other parts of the world and assumptions of measurement techniques are most often met (Lasker, 1985). Given this ease of collection, it is not surprising that numerous studies of isonymy and inbreeding have been performed on historical and contemporary human populations.Many studies of inbreeding, however, have focused on the presentation of inbreeding levels in one or more populations without a thorough analysis of the determinants of inbreeding. In theory, inbreeding levels in a population should increase to reach an equi-