“…The RAG2 gene encodes components of the recombinase involved in recombination of immunoglobin and T-cell receptor genes and appears as conserved single copies in all examined vertebrates (Hansen and Kaattari, 1996;Willett et al, 1997). The RAG2 gene has been widely used to evaluate intrageneric and intraspeciWc relationships (Baker et al, 2000;Clements et al, 2004;Hardman, 2004;Lewis-Oritt et al, 2001;Lovejoy and Collette, 2001), and it is also used to reveal higher-level phylogenetic relationships (Brinkmann et al, 2004;Calcagnotto et al, 2005). Although the considerable morphological variability of East Asian cyprinids represents a challenge to phylogenetic analyses based on morphology, we included representative species from all hypothesized subfamilies (Chen, 1998) in our present molecular analysis.…”