Relationships among cell attachment, spreading, cytoskeletal organization, and migration rate for anchorage-dependent cells on model surfaces

Webb, K. E.; Hlady, Vladimir; Tresco, Patrick A.

doi:10.1002/(sici)1097-4636(20000305)49:3<362::aid-jbm9>3.0.co;2-s

Cited by 231 publications

(157 citation statements)

References 33 publications

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“…In the case of SAMs, differences in cell attachment [39] as well as differences in myoblast differentiation [2] could be attributed to film chemistry and/ or wettability. The nature of the functional groups was indeed found to be the most important factor [40] . For all these reasons, small changes in (PLL/HA) film chemistry and wettability upon cross-linking are likely to play a minor role in the observed differences in cell behavior.…”

Section: Relative Importance Of Film Stiffness Roughness and Wettabimentioning

confidence: 92%

Polyelectrolyte Multilayer Films of Controlled Stiffness Modulate Myoblast Cell Differentiation

Ren

Crouzier

Roy

et al. 2008

Adv Funct Materials

244

263

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Beside chemical properties and topographical features, mechanical properties of gels have been recently demonstrated to play an important role in various cellular processes, including cell attachment, proliferation, and differentiation. In this work, we used multilayer films made of poly (L-lysine)/Hyaluronan (PLL/HA) of controlled stiffness to investigate the effects of mechanical properties of thin films on skeletal muscle cells (C2C12 cells) differentiation. Prior to differentiation, cells need to adhere and proliferate in growth medium. Stiff films (E 0 > 320 kPa) promoted formation of focal adhesions and organization of the cytoskeleton as well as an enhanced proliferation, whereas soft films were not favorable for cell anchoring, spreading or proliferation. Then C2C12 cells were switched to a low serum containing medium to induce cell differentiation, which was also greatly dependent on film stiffness. Although myogenin and troponin T expressions were only moderately affected by film stiffness, the morphology of the myotubes exhibited striking stiffness-dependent differences. Soft films allowed differentiation only for few days and the myotubes were very short and thick. Cell clumping followed by aggregates detachment could be observed after ~2 to 4 days. On stiffer films, significantly more elongated and thinner myotubes were observed for up to ~ 2 weeks. Myotube striation was also observed but only for the stiffer films. These results demonstrate that film stiffness modulates deeply adhesion, proliferation and differentiation, each of these processes having its own stiffness requirement.

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Section: Relative Importance Of Film Stiffness Roughness and Wettabimentioning

confidence: 92%

Polyelectrolyte Multilayer Films of Controlled Stiffness Modulate Myoblast Cell Differentiation

Ren

Crouzier

Roy

et al. 2008

Adv Funct Materials

244

263

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“…The cells might spread up to 100 μm in size on the APTMS-coated substrates (Fig. 3c), and this effect is attributed to the chemical properties of the NH 2 + functional group of APTMS (Webb et al, 2000).…”

Section: Morphology Of Cho Cells On Different Substratesmentioning

confidence: 99%

Cell adhesion, morphology and biochemistry on nano-topographic oxidized silicon surfaces

Yang

Huang²,

Shen³

et al. 2010

eCM

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Manipulating an incorporated scaffold to direct cell behaviors play a key role in tissue engineering. In this study, we developed novel nano-topographic oxidized silicon nanosponges capable of being modified with various chemicals of a few nm in thickness to gain further insight into the fundamental biology of cell-environment interactions in vitro. A wet etching technique was applied to fabricate the silicon nanosponges in a high-throughput manner and was followed by vapor deposition of various organo-silane chemicals to enable self-assembly on the surfaces of the silicon nanosponges. When Chinese hamster ovary cells were cultured on these chemically modified nano-topographic structures, they displayed distinct morphogenesis, adherent responses, and biochemical properties in comparison with those of their planar oxidized silicon counterparts. There were predominant nano-actin punches and slender protrusions formed while cells were cultured on the nano-topographic structures, indicating that cell behaviors can be influenced by the physical characteristic derived from nano-topography, in addition to the hydrophobicity of contact surfaces. This study demonstrates potential applications of these nanotopographic biomaterials for controlling cell development in tissue engineering as well as in basic cell biology research.

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“…Besides, cell migration also can be dominated by the chemical and physical properties of the substrates. For example, Webb et al found that the migration rate of MC3T3-E1 osteoblasts is significantly slower on the thiol surface compared with the oxidized thiol, quaternary amine and methyl surfaces [14]. Pelham & Wang [6] and Lo et al [15] showed that cell motility can be governed purely by the substrate rigidity.…”

Section: Introductionmentioning

confidence: 99%

Influences of surface chemistry and swelling of salt-treated polyelectrolyte multilayers on migration of smooth muscle cells

Han

Mao

et al. 2012

J. R. Soc. Interface.

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The cell migration plays a crucial role in a variety of physiological and pathological processes and can be regulated by the cell -substrate interactions. We found previously that the poly (sodium 4-styrenesulphonate) (PSS)/poly(diallyldimethylammonium) chloride (PDADMAC) multilayers post-treated in 1-5 M NaCl solutions result in continuous changes of their physicochemical properties such as thickness, chemical composition, surface charge, swelling ratio and wettability. In this study, the responses of human smooth muscle cells (SMCs) on these salt-treated multilayers, particularly the governing factors of cellular migration that offer principles for designing therapeutics and implants, were disclosed. The cell migration rate was slowest on the 3 M NaCl-treated multilayers, which was comparable with that on tissue culture plates, but it was highest on 5 M NaCl-treated multilayers. To elucidate the intrinsic mechanisms, cell adhesion, proliferation, adhesion and related gene expressions were further investigated. The SMCs preferred to attach, spread and proliferate on the PSS-dominated surfaces with well-organized focal adhesion and actin fibres, especially on the 3 M NaCl-treated multilayers, while were kept round and showed low viability on the PDADMAC-dominated surfaces. The relative mRNA expression levels of adhesion-related genes such as fibronectin, laminin and focal adhesion kinase, and migration-related genes such as myosin IIA and Cdc42 were compared to explain the different cellular behaviours. These results reveal that the surface chemistry and the swelling of the salt-treated multilayers govern the cell migration behaviours.

show abstract

Relationships among cell attachment, spreading, cytoskeletal organization, and migration rate for anchorage-dependent cells on model surfaces

Cited by 231 publications

References 33 publications

Polyelectrolyte Multilayer Films of Controlled Stiffness Modulate Myoblast Cell Differentiation

Polyelectrolyte Multilayer Films of Controlled Stiffness Modulate Myoblast Cell Differentiation

Cell adhesion, morphology and biochemistry on nano-topographic oxidized silicon surfaces

Influences of surface chemistry and swelling of salt-treated polyelectrolyte multilayers on migration of smooth muscle cells

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