Relationship of vaginal discharge characteristics evaluated by Metricheck device to metabolic status in postpartum dairy cows

Kawashima, Chiho; Suwanai, Masashi; Honda, Toshihiko; Teramura, Makoto; Kida, Katsuya; Hanada, Masaaki; Miyamoto, Akio; Matsui, Motozumi

doi:10.1111/rda.13255

Cited by 6 publications

(14 citation statements)

References 36 publications

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“…Both groups showed smooth recovery compared to previous studies (Kawashima et al, 2018;Stephen et al, 2019). The PMN percentages at 3 and 5 weeks were consistent with those of previous reports (Gilbert & Santos, 2016;Stephen et al, 2019) and were comparable between the RPL and CON groups.…”

Section: Itemsupporting

confidence: 91%

Supplementation of rumen‐protected lysine during the close‐up period improves vaginal discharge clearance in Holstein dairy cows

Kawashima,

Hayakawa,

Taniguchi

et al. 2024

Reprod Domestic Animals

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We aimed to evaluate the effects of rumen‐protected lysine (RPL) supplementation during the close‐up period on uterine involution and the resumption of ovarian function in dairy cows. Fifty‐two multiparous Holstein cows were categorized based on parity and expected calving date and randomly assigned to the RPL or control (CON) groups. The RPL group received 80 g of RPL daily from day 21 before the expected calving date until parturition. Blood samples were obtained twice weekly from pre‐supplementation to 6 weeks postpartum. The onset of luteal activity postpartum was determined via ultrasonography twice weekly for up to 6 weeks postpartum. Uterine involution was tracked at 3 and 5 weeks postpartum through the vaginal discharge score, percentage of polymorphonuclear cells (PMN) in endometrial cytology samples, presence of intrauterine fluid, and gravid horn diameter via ultrasonography. Before supplementation, the RPL group showed amino acid imbalance, which was improved by RPL supplementation. There were no significant differences in the onset of luteal activity, percentage of PMN, intrauterine fluid, or the diameter of the uterine horn between the two groups. The vaginal discharge score in the RPL group decreased from 3 to 5 weeks postpartum, whereas that in the CON groups did not decrease. The number of cows with clinical endometritis was lower in the RPL group. Overall, RPL supplementation during the close‐up period enhanced vaginal discharge clearance, potentially averting clinical endometritis, but did not affect the first ovulation in dairy cows.

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Section: Itemsupporting

confidence: 91%

Supplementation of rumen‐protected lysine during the close‐up period improves vaginal discharge clearance in Holstein dairy cows

Kawashima,

Hayakawa,

Taniguchi

et al. 2024

Reprod Domestic Animals

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“…Others have reported that endometrial epithelial cells exhibited reduced PGF 2α production when treated with exosomes isolated from plasma of cows with a uterine infection compared with those treated with exosomes isolated from healthy cows (Almughlliq et al, 2017). Additionally, concentrations of PGFM tended to be lower in cows with endometritis (based on Metricheck reading) than healthy cows (Kawashima et al, 2018). Differences between these studies could be due to differences in cow breeds, methods of classifica-tion of endometritis or uterine infection, and in vitro versus in vivo analysis.…”

Section: Discussionmentioning

confidence: 97%

Relationships between prostaglandin concentrations, a single nucleotide polymorphism in HSD17B12, and reproductive performance in dairy cows

Juengel

Mosaad

Mitchell

et al. 2022

Journal of Dairy Science

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Prostaglandins are involved in multiple processes important for fertility, with previous work in mice highlighting a potential role for the HSD17B12 gene in prostaglandin biosynthesis. This study aimed to determine the associations among circulating prostaglandin concentrations, a missense SNP in the HSD17B12 gene predicted to disrupt protein function, and fertility traits in first-lactation Holstein-Friesian dairy cows. We used a study population of approximately 500 animals specifically bred to have either a positive (POS, +5%) or negative (NEG, −5%) genetic merit for fertility (FertBV). Genotypes of a previously identified SNP (rs109711583) in HSD17B12 were determined, with 116 animals genotyped as AA, 215 genotyped as AG, and 153 genotyped as GG. Plasma concentrations of prostaglandin E 2 and the PGF 2α metabolite PGFM were determined at 3 time points (12 mo of age, 4 d postpartum, and 5 wk postpartum during first lactation) in a selection of animals with AA and GG genotypes from both the POS and NEG FertBV groups (n = 33-40 in each genotype for each FertBV group). Binary reproductive traits (yes or no) examined included submission for artificial breeding in the first 3 or 6 wk of the seasonal breeding period; conception to first service; conception during the first 6 wk of the breeding period; and pregnant at the end of the breeding period. Uterine health at 6 wk after calving was examined by evaluating the percentage of polymorphonuclear leukocytes following uterine cytology and by scoring vaginal discharge based on the presence of purulent material. The 3-wk submission rate was increased in animals that carried the G allele of the missense SNP in HSD17B12, but no differences were present among genotypes for 6-wk submission rate. The trait was additive, with each increase of the G allele increasing the 3-wk submission rate by 6 to 7%. We did not observe any consistent associations between SNP alleles and circulating PGE 2 concentrations; however, a complex 3-way interaction among time, fertility group, and SNP allele was present for PGFM concentrations. Plasma concentrations of PGE 2 were increased approximately 40% at 5 wk postpartum in animals that were submitted for breeding within 3 or 6 wk of the start of the breeding season, and in those that conceived during the first 6 wk of breeding, compared with those that did not. Plasma concentrations of PGFM were decreased approximately 20% in those animals that conceived to their first service and tended to be decreased in animals that were pregnant at the end of the breeding period, compared with those that were not. In summary, associations were observed between the SNP in HSD17B12 and submission rate by d 21 of the breeding season, as well as between circulating prostaglandin concentrations and fertility traits, but the SNP was not consistently linked to changes in prostaglandin concentrations. Thus, the association between submission rate by d 21 of the breeding season and the SNP in HSD17B12 were unlikely driven by changes in prostaglandins.

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“…PGF 2α is necessary for the process of spontaneous uterine involution [19][20][21]. Kawashima et al [11] reported that the blood concentration of the PGF 2α metabolite 13,14-dihydro-15-keto-PGF 2α (PGFM) tended to be higher in cows with delayed spontaneous uterine involution and lower in cows with endometritis than in healthy cows during W3. The cows with normal uterine involution showed a negative correlation between the blood PGFM level and the time to complete uterine involution.…”

Section: Discussionmentioning

confidence: 99%

“…The VDS was determined at W2, W3, and W5 using a Metricheck device (Simcro Tech Ltd., Hamilton, New Zealand) that comprised a stainless-steel rod and a silicon cup. The VDS assessment method was similar to the method described by [11] with slight modifications as follows: VDS1 = clear or translucent mucus; VDS2 = mucus containing flecks of white pus or off-white purulent material; VDS3 = discharge containing <50% purulent material; and VDS4 = discharge containing >50% purulent material.…”

Section: Experiments 1: Comparison Of the Effects Of Intrauterine Infusion Of A Chitosan Solution Versus Intramuscular Administration Of mentioning

confidence: 99%

Effects of Intrauterine Infusion of a Chitosan Solution on Recovery and Subsequent Reproductive Performance of Early Postpartum Dairy Cows with Endometritis: A Pilot Field Trial

Okawa

Wijayagunawardane

Vos

et al. 2021

Animals

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This study investigated the efficacy of intrauterine infusion of a chitosan solution (CHT) on uterine recovery in early postpartum dairy cows with or without endometritis, and their subsequent reproductive performance. In Experiment 1, cows with endometritis at 3 weeks postpartum were administered CHT (n = 5) and prostaglandin F2α (PGF2α) (n = 4). Untreated cows (n = 7) served as the control group. In Experiment 2, 18 cows with a normally recovered uterus at the fresh cow check (mean, 35 days postpartum) were assigned to the CHT (n = 10) and control (n = 8) groups, and intrauterine infusion was conducted in the CHT group. Overall, in Experiment 1, the percentage of polymorphonuclear leukocytes significantly declined in the CHT group (32.3 ± 10.2 to 5.5 ± 2.4, p < 0.05) from week 3 to week 5, but no decline occurred in the PGF2α and control groups. In Experiment 2, the CHT and control groups showed no significant differences in reproductive parameters, suggesting the absence of adverse effects of CHT on fertility. These results suggest that intrauterine infusion of CHT in the early postpartum period effectively accelerates uterine recovery from endometritis and might be a suitable replacement for PGF2α administration.

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Relationship of vaginal discharge characteristics evaluated by Metricheck device to metabolic status in postpartum dairy cows

Cited by 6 publications

References 36 publications

Supplementation of rumen‐protected lysine during the close‐up period improves vaginal discharge clearance in Holstein dairy cows

Supplementation of rumen‐protected lysine during the close‐up period improves vaginal discharge clearance in Holstein dairy cows

Relationships between prostaglandin concentrations, a single nucleotide polymorphism in HSD17B12, and reproductive performance in dairy cows

Effects of Intrauterine Infusion of a Chitosan Solution on Recovery and Subsequent Reproductive Performance of Early Postpartum Dairy Cows with Endometritis: A Pilot Field Trial

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