Relationship of the Levels of microRNA Gene Methylation with the Level of Their Expression and Pathomorphological Characteristics of Breast Cancer

Филиппова, Е. А.; Пронина, И. В.; Лукина, С. С.; Kazubskaya, T. P.; Брага, Э. А.; Бурденный, А. М.; Логинов, В. И.

doi:10.1007/s10517-021-05312-2

Cited by 3 publications

(3 citation statements)

References 11 publications

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“…Moreover, miR129 promoter methylation was also reported in gastric cancer, hematological malignancies, and metastatic colorectal cancer 16–18 . In breast cancer, it is significantly associated with tumor progression and poor prognosis biomarker 34,35 . Specifically, cg14416371, a probe within miR129 CpG island which overlaps the one selected for our study, was previously used to identify pancreatic cancer 36 .…”

Section: Discussionmentioning

confidence: 90%

“… 16 , 17 , 18 In breast cancer, it is significantly associated with tumor progression and poor prognosis biomarker. 34 , 35 Specifically, cg14416371, a probe within miR129 CpG island which overlaps the one selected for our study, was previously used to identify pancreatic cancer. 36 Furthermore, in silico data using two methylation‐based data sets (GSE52068 and GSE62336) identified miR129‐2 as a candidate hypermethylated gene in nasopharyngeal carcinoma.…”

Section: Discussionmentioning

confidence: 99%

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DNA methylation biomarkers accurately detect esophageal cancer prior and post neoadjuvant chemoradiation

et al. 2023

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Background Esophageal cancer (ECa) is associated with high mortality, mostly due to late diagnosis, precluding curativeintent surgery. Hence, neoadjuvant chemoradiation (ChRT) is recommended in most patients regardless of histological subtype. A proportion of these patients, however, achieve complete disease remission and might be spared of radical surgery. The lack of reliable, minimally invasive biomarkers able to detect post‐ChRT disease persistence is, nonetheless, a major drawback. We have previously shown that miRNA promotor methylation enables accurate cancer detection in tissues and liquid biopsies but has been seldom explored in ECa patients. Aims Herein, we sought to unveil and validate novel candidate biomarkers able to detect ECa prior and post ChRT. Materials and Methods Promoter methylation of miR129‐2, miR124‐3 and ZNF569 was assessed, using quantitative methylation‐specific PCR (qMSP), in tissue samples from normal esophagus, treatment‐naïve and post‐ChRT ECa, as well as in liquid biopsies from ECa patients. Results All genes disclosed significantly different promoter methylation levels between ECa and normal esophagus, accurately detecting post‐ChRT disease, especially for adenocarcinoma. Remarkably, miR129‐2me/ZNF569me methylation panel identified ECa in liquid samples with 53% sensitivity and 87% specificity. Discussion MiR129‐2me, miR124‐3me and ZNF569me accurately discriminate ECa, either pre‐ or post‐ChRT, from normal tissue, enabling ECa detection. Furthermore, circulalting methylation‐based biomarkers are promising minimally invasive tools to detect post‐ChRT residual ECa. Conclusion Overall, our results encourage the use of miRNA methylation biomarkers as accurate ECa detection tools as a novel approach for ChRT response monitoring.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

DNA methylation biomarkers accurately detect esophageal cancer prior and post neoadjuvant chemoradiation

et al. 2023

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“…miR-127-5p is a miRNA that has been shown to mediate the polarization of M1 macrophages, inhibiting the migration of cancer cells and polarizing macrophages into the M1 phenotype [ 20 ]. miR-127-5p was downregulated in breast cancers [ 21 ], cervical cancers [ 22 ], gastric cancers [ 23 ], and many other tumor types [ 24 ]. miR-127-5p mediated the phenotype of cutaneous squamous cell carcinoma cells by binding to the ADCY7 gene [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

miR-127-5p Targets JAM3 to Regulate Ferroptosis, Proliferation, and Metastasis in Malignant Meningioma Cells

2022

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Objective. Meningiomas are one of the most common primary tumors of the central nervous system. Most of them are benign and can be cured by surgery, while a few meningiomas are malignant. Ferroptosis gene characteristics might be associated with drug therapy and survival in patients with clinically aggressive, unresectable meningiomas. This study explored the mechanism of differentially expressed miRNAs and ferroptosis in meningioma to provide a new reference to treat meningioma. Methods. Bioinformatics analysis of differential miRNA profiles and functions in patients with meningioma was performed. The contents of lactate dehydrogenase (LDH), malondialdehyde (MDA), and Fe2+ were determined. Reactive oxygen species (ROS) values, as well as cell cycle changes, were analyzed by flow cytometry. The targets of miR-127-5p and JAM3 were detected by dual luciferase assays. Cell counting kit-8 (CCK8) and Transwell assays were used to analyze cell activity. Ki67 expression was analyzed by immunohistochemistry. Expression levels of miR-127-5p and JAM3 were analyzed by RT-qPCR. GPX4 expression was quantified by western blotting. Results. miR-127-5p was expressed at low levels in IOMM-Lee cells, while JAM3 was highly expressed in IOMM-Lee cells. A dual luciferase assay demonstrated that miR-127-5p could target JAM3. Upregulation of miR-127-5p in IOMM-Lee cells resulted in cell cycle arrest and inhibition of cell activity. Upregulation of miR-127-5p increased LDH, MDA, and ROS levels and Fe2+ content and inhibited the expression of GPX4 protein. Upregulation of JAM3 reversed the results of miR-127-5p upregulation. Conclusion. miR-127-5p regulated meningioma formation and ferroptosis through JAM3, providing insights for the development of new treatments for meningioma.

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The Role of Methylation of a Group of microRNA Genes in the Pathogenesis of Metastatic Renal Cell Carcinoma

et al. 2023

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Relationship of the Levels of microRNA Gene Methylation with the Level of Their Expression and Pathomorphological Characteristics of Breast Cancer

Cited by 3 publications

References 11 publications

DNA methylation biomarkers accurately detect esophageal cancer prior and post neoadjuvant chemoradiation

DNA methylation biomarkers accurately detect esophageal cancer prior and post neoadjuvant chemoradiation

miR-127-5p Targets JAM3 to Regulate Ferroptosis, Proliferation, and Metastasis in Malignant Meningioma Cells

The Role of Methylation of a Group of microRNA Genes in the Pathogenesis of Metastatic Renal Cell Carcinoma

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