Relationship of the internal solute composition to the salt tolerance of <i>Halomonas elongata</i>

Vreeland, Russell H.; Mierau, Bradley D.; Litchfield, Carol D.; Martin, Eugene L.

doi:10.1139/m83-066

Cited by 48 publications

(20 citation statements)

References 5 publications

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“…We determined a maximum total solute concentration of 20-27% of the cellular dry weight. The slightly lower values at elevated salt concentrations and temperatures are probably due to an increased amino acid pool and higher internal ion concentrations as have been reported before (Vreeland et al, 1983 ;Sadler et al, 1980).…”

Section: Discussionsupporting

confidence: 63%

“…This would be equivalent to a cytoplasmic concentration of 2.25 M-ectoine. Added up with about 400 pmol of cations and amino acids (Vreeland et al, 1983), and assuming equivalent amounts of inorganic ions, this would amount to a total solute concentration of 3-05 M, which is close to achieving osmotic equilibrium.…”

Section: Discussionmentioning

confidence: 99%

“…Studies on the cellular ion content of halotolerant and halophilic aerobic heterotrophic micro-organisms have revealed that the intracellular ion concentration is too low to balance the osmotic pressure, as shown for Pseudomonas halosaccharolytica (Matheson et al, 1976), Halomonas elongata (Vreeland et al, 1983) and Paracoccus halodenitrijicans (Sadler et al, 1980). As no organic solutes have been detected in concentrations sufficiently high to account for a function as compatible solutes, the osmoadaptive mechanism remains obscure.…”

Section: Introductionmentioning

confidence: 99%

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The spectrum of compatible solutes in heterotrophic halophilic eubacteria of the family Halomonadaceae

Wohlfarth

Severin

Galinski

1990

Journal of General Microbiology

139

101

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A new family, the Hafomonadaceae, has recently been proposed for members of the genera Deleya and Halornonas. The three strains investigated, Deleya haiuphila, Halomonas elongata and Flavobacterium halmephilum (reclassified as H. hahophila), are aerobic heterotrophic micro-organisms exhibiting an extreme salt tolerance. The major organic osmoregulatory solutes of these organisms were examined using 3C-nuclear magnetic resonance spectroscopy. The relative proportions of the solutes varied with respect to salt concentration, temperature and carbon source. The recently described amino acid ectoine was found to be a dominant solute. For the first time it could be shown for halophilic eubacteria that the intracellular concentration of solutes is sufficient to balance the osmotic pressure of the medium. Thus, there is no need to postulate a hypo-osmotic cytoplasm.

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Section: Discussionsupporting

confidence: 63%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The spectrum of compatible solutes in heterotrophic halophilic eubacteria of the family Halomonadaceae

Wohlfarth

Severin

Galinski

1990

Journal of General Microbiology

139

101

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“…novas et al, , 1997(Ca! novas et al, , 1998aGo$ ller et al, 1998 ;Hart & Vreeland, 1988 ;Martin et al, 1983 ; Vargas et al, 1995 ;Vreeland et al, 1983). In the For our study, we used the strains listed in Table 1.…”

mentioning

confidence: 99%

Chromohalobacter salexigens sp. nov., a moderately halophilic species that includes Halomonas elongata DSM 3043 and ATCC 33174.

Arahal¹,

Garcı́a²,

Vargas³

et al. 2001

International Journal of Systematic and Evolutionary Microbiology

131

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NOTE Chromohalobacter salexigens sp. nov., a moderately halophilic species that includes Halomonas elongata DSM 3043 and ATCC 33174 Departamento de Microbiologı! a y Parasitologı! a, Two strains that were originally isolated and characterized as members of the moderately halophilic species Halomonas elongata, strains DSM 3043 (l 1H11) and ATCC 33174 (l 1H15), were studied in detail. Their complete 16S rRNA sequences were determined and, when compared to sequences available from the databases, they showed a close phylogenetic relationship to Chromohalobacter marismortui. In addition, DNA-DNA hybridization experiments showed that both strains are members of the same species, but their DNA relatedness to the type strains of Halomonas elongata, ATCC 33173 T , and Chromohalobacter marismortui, ATCC 17056 T , is very low. Phenotypically, the two strains showed very similar features, related to those of Chromohalobacter, but clear differences were found between these two strains and Chromohalobacter marismortui. On the basis of these data, it is proposed that Halomonas elongata DSM 3043 and ATCC 33174 should be included in a new species of the genus Chromohalobacter, Chromohalobacter salexigens sp. nov. The type strain is DSM 3043 T Chromohalobacter salexigens sp. nov. ornithine , -proline and -serine. The following compounds are not utilized as sole sources of carbon, nitrogen and energy : -alanine, creatine, -methion-ine, putrescine, sarcosine, -threonine and -valine. The GjC content of the DNA is 64n2-66n0 mol% (T m method). The EMBL accession numbers of the 16S rRNA gene sequences of strains DSM 3043 T and ATCC 33174 determined in this study are AJ295146 and AJ295147, respectively. Isolated from salterns. The type strain is DSM 3043 T

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“…On the other hand, slightly and moderately halophilic microorganisms had the tendency to increase the internal Nay concentrations in proportion to the NaCI concentration in the medium (15,22,26,28). Internal K t concentrations of these halophiles, however, was little affected by the external NaCI concentrations (27), except for some bacteria (20,28). In this respect, it seems to be of interest to examine quantitatively the changes of cation concentrations in the cells grown in the presence of salts other than NaCI.…”

Section: Nagatamentioning

confidence: 99%

Internal cation concentrations of the halotolerant bacterium Brevibacterium sp. in response to the concentrations and species of external salts.

Nagata

Ogawa

Mimura

1991

J. Gen. Appl. Microbiol.

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Internal cation concentrations of the halotolerant bacterium Brevibacterium sp. were investigated, with the cells grown in a complex media supplemented with nine kinds of salts. With increases in the external NaCI concentration, the amount of Na+ ion in the cells increased, while the intracellular concentration of the K+ ion remained almost constant (about 300 mM). The same tendency was observed when NaCI was replaced with KCI, although the Na+ ion was very low (about 15 mM). On the contrary, intracellular concentrations of Mg2+ and Ca2+ decreased slightly with the increase in NaCI or KCI concentrations in the medium. When the RbCI concentration in the medium was increased to more than 100 mM, Rb + ions in the cells increased about one hundred times, 550-600 mM, while K+ ions decreased to one-tenth. The same experiment was resumed using the chemically defined medium. We observed two different phenomena. First, the intracellular Na+ content (about 200 mM) was more than ten-fold higher than that of the cells grown in the complex medium. Second, the release of K+ ions from the cells at alkaline pH was not as remarkable as that in the complex medium. The release of K+ ions observed in the complex medium was correlated with the addition of 50 mM diethanolamine-hydrochloride for adjusting medium pH at 9.0. Na+ H+ Concerning the energetic systems -rich environments and require Na+ antiporter via H+ pump (3,13,16) of halophilic bacteria which inhabit ions for growth, the importance of Na+l and respiration-dependent primary Na+

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Relationship of the internal solute composition to the salt tolerance of Halomonas elongata

Cited by 48 publications

References 5 publications

The spectrum of compatible solutes in heterotrophic halophilic eubacteria of the family Halomonadaceae

The spectrum of compatible solutes in heterotrophic halophilic eubacteria of the family Halomonadaceae

Chromohalobacter salexigens sp. nov., a moderately halophilic species that includes Halomonas elongata DSM 3043 and ATCC 33174.

Internal cation concentrations of the halotolerant bacterium Brevibacterium sp. in response to the concentrations and species of external salts.

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