Relationship of sperm DNA fragmentation, apoptosis and dysfunction of mitochondrial membrane potential with semen parameters and ART outcome after intracytoplasmic sperm injection

Sharbatoghli, Mina; Valojerdi, Mojtaba Rezazadeh; Amanlou, Massoud; Khosravi, Fariba; Jafarabadi, Mohammad Asghari

doi:10.1007/s00404-012-2440-1

Cited by 45 publications

(31 citation statements)

References 39 publications

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“…Several investigators observed lower sDNAfrag in men with higher sperm concentration, total motility, progressive motility and normal morphology (Belloc et al, 2014;Moskovtsev et al, 2009;Oosterhuis et al, 2000;Shen et al, 2002;Zhang et al, 2008), whereas others showed that these parameters are not correlated with sDNAfrag (Ricci et al, 2002). However, after use of sperm-preparation techniques, results appear more consistent, several reports finding that after swim-up the percentage of sDNAfrag was negatively correlated with motility and morphology (Lopes et al, 1998;Sharbatoghli et al, 2012), with the same results observed after the use of DGC (Sakkas et al, 1999). In relation to sperm pathologies, using DGC followed by wash and swim-up, a significant correlation between the degree of sDNAfrag and asthenozoospermia was not found, but a significantly higher percentage of sDNAfrag was reported in men with teratozoospermia (Mehdi et al, 2009).…”

Section: Tunel+ H-tunel+ Tunel+ H-tunel+ Concentrationmentioning

confidence: 76%

Sperm DNA fragmentation is related to sperm morphological staining patterns

Sá

Cunha

Rocha

et al. 2015

Reproductive BioMedicine Online

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Section: Tunel+ H-tunel+ Tunel+ H-tunel+ Concentrationmentioning

confidence: 76%

Sperm DNA fragmentation is related to sperm morphological staining patterns

Sá

Cunha

Rocha

et al. 2015

Reproductive BioMedicine Online

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“…These studies were excluded from meta-analysis, because no cut-off values were used so a 2x2 table could not be constructed from the data. Some of these studies found an association between sperm DNA fragmentation and pregnancy [83,92,93,97,104,107], while others did not find an association between sperm DNA fragmentation and conception [47,116]. In conclusion, we think that the best way to judge the value of DNA fragmentation tests is by their predictive capacity for the outcome of interest, ongoing pregnancy.…”

Section: Discussionmentioning

confidence: 95%

“…We found 21 studies reported on the SCSA [103,110–112,114,117,118,123–136], 18 studies on the SCD test [97,98,101,102,106–109,113,115,116,120,122,137–141], 18 studies reported on the TUNEL assay [92–95,96,99,100,104,119,134,142–149] and seven studies reported on the alkaline Comet assay [95,105,121,134,150–152]. …”

Section: Resultsmentioning

confidence: 99%

Measuring Sperm DNA Fragmentation and Clinical Outcomes of Medically Assisted Reproduction: A Systematic Review and Meta-Analysis

et al. 2016

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Sperm DNA fragmentation has been associated with reduced fertilization rates, embryo quality, pregnancy rates and increased miscarriage rates. Various methods exist to test sperm DNA fragmentation such as the sperm chromatin structure assay (SCSA), the sperm chromatin dispersion (SCD) test, the terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate nick end labelling (TUNEL) assay and the single cell gel electrophoresis (Comet) assay. We performed a systematic review and meta-analysis to assess the value of measuring sperm DNA fragmentation in predicting chance of ongoing pregnancy with IVF or ICSI. Out of 658 unique studies, 30 had extractable data and were thus included in the meta-analysis. Overall, the sperm DNA fragmentation tests had a reasonable to good sensitivity. A wide variety of other factors may also affect the IVF/ICSI outcome, reflected by limited to very low specificity. The constructed hierarchical summary receiver operating characteristic (HSROC) curve indicated a fair discriminatory capacity of the TUNEL assay (area under the curve (AUC) of 0.71; 95% CI 0.66 to 0.74) and Comet assay (AUC of 0.73; 95% CI 0.19 to 0.97). The SCSA and the SCD test had poor predictive capacity. Importantly, for the TUNEL assay, SCD test and Comet assay, meta-regression showed no differences in predictive value between IVF and ICSI. For the SCSA meta-regression indicated the predictive values for IVF and ICSI were different. The present review suggests that current sperm DNA fragmentation tests have limited capacity to predict the chance of pregnancy in the context of MAR. Furthermore, sperm DNA fragmentation tests have little or no difference in predictive value between IVF and ICSI. At this moment, there is insufficient evidence to recommend the routine use of sperm DNA fragmentation tests in couples undergoing MAR both for the prediction of pregnancy and for the choice of treatment. Given the significant limitations of the evidence and the methodological weakness and design of the included studies, we do urge for further research on the predictive value of sperm DNA fragmentation for the chance of pregnancy after MAR, also in comparison with other predictors of pregnancy after MAR.

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“…Although Degheidy et al [41] reported that post-MACS samples showed no difference in sperm motility (80.6%±6.9%) compared with control samples (80.9%±7.7%), de Vantery Arrighi et al [42] reported that the progressive sperm motility of post-MACS semen samples (67.9%±3.8%, p <0.05) was significantly better than pre-MACS control samples (48.4%±2.4%). Additionally, other studies have reported a significant inverse correlation between apoptosis and progressive motility [434445]. Therefore, the positive effect of MACS on the isolation of sperm with high DNA integrity may result from the selection of non-apoptotic sperm with progressive motility.…”

Section: Discussionmentioning

confidence: 99%

Efficient isolation of sperm with high DNA integrity and stable chromatin packaging by a combination of density-gradient centrifugation and magnetic-activated cell sorting

Chi¹,

Kwak²,

Kim³

et al. 2016

Clin Exp Reprod Med

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ObjectiveThis study was carried out to investigate the correlations of the sperm DNA fragmentation index (DFI) with semen parameters and apoptosis, and to investigate the effects of density-gradient centrifugation (DGC) and magnetic-activated cell sorting (MACS) on reducing the proportion of sperm with DNA fragmentation and protamine deficiency.MethodsSemen analysis and a sperm DNA fragmentation assay were performed to assess the correlations between semen parameters and the DFI in 458 semen samples. Sperm with progressive motility or non-apoptosis were isolated by DGC or MACS, respectively, in 29 normozoospermic semen samples. The effects of DGC or MACS alone and of DGC and MACS combined on reducing the amount of sperm in the sample with DNA fragmentation and protamine deficiency were investigated.ResultsThe sperm DFI showed a significant correlation (r=–0.347, p<0.001) with sperm motility and morphology (r=–0.114, p<0.05) but not with other semen parameters. The DFI (11.5%±2.0%) of semen samples was significantly reduced by DGC (8.1%±4.1%) or MACS alone (7.4%±3.9%) (p<0.05). The DFI was significantly further reduced by a combination of DGC and MACS (4.1%±1.3%, p<0.05). Moreover, the combination of DGC and MACS (1.6%±1.1%, p<0.05) significantly reduced the protamine deficiency rate of semen samples compared to DGC (4.4%±3.2%) or MACS alone (3.4%±2.2%).ConclusionThe combination of DGC and MACS may be an effective method to isolate high-quality sperm with progressive motility, non-apoptosis, high DNA integrity, and low protamine deficiency in clinical use.

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Relationship of sperm DNA fragmentation, apoptosis and dysfunction of mitochondrial membrane potential with semen parameters and ART outcome after intracytoplasmic sperm injection

Abstract: In the present study; DF, apoptosis and dysfunction of sperm MMP indicated negative relationship with sperm parameters. Although there was a negative correlation between early apoptosis and pregnancy rate, no significant correlation was observed between these parameters and ICSI outcomes.

Cited by 45 publications

References 39 publications

Sperm DNA fragmentation is related to sperm morphological staining patterns

Sperm DNA fragmentation is related to sperm morphological staining patterns

Measuring Sperm DNA Fragmentation and Clinical Outcomes of Medically Assisted Reproduction: A Systematic Review and Meta-Analysis

Efficient isolation of sperm with high DNA integrity and stable chromatin packaging by a combination of density-gradient centrifugation and magnetic-activated cell sorting

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