Relationship of ovarian stimulation response with vascular endothelial growth factor and degree of granulosa cell apoptosis

Quintana, Ramiro; Kopcow, L.; Marconi, Guillermo; Sueldo, Carlos; Speranza, G.; Barañao, Rosa Inés

doi:10.1093/humrep/16.9.1814

Cited by 34 publications

(12 citation statements)

References 32 publications

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“…A possible hypothesis [33] for this finding is that granulosa cells exposed to different oxygen conditions in vitro may suffer hypoxia and stimulate VEGF production [19]. In contrast, high VEGF levels produced by granulosa cells in culture are detected in patients with elevated plasma estradiol concentrations, with a larger number of follicles and retrieved oocytes and higher fertilization and pregnancy rates [34,35].…”

Section: Discussionmentioning

confidence: 99%

Vascular endothelial growth factor in the plasma, follicular fluid and granulosa cells of women with endometriosis submitted toin vitrofertilization – a pilot study

Oliveira¹,

Abreu²,

Ferriani³

et al. 2005

Gynecological Endocrinology

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Vascular endothelial growth factor (VEGF), a potent angiogenic factor that is altered in endometriosis, supports the immunological mechanism involved in this disease. The aim of the present study was to assess VEGF concentration in the plasma, follicular fluid (FF) and culture medium (CM) of granulosa cells from patients with endometriosis submitted to in vitro fertilization (IVF). A case-control study was conducted on 14 patients with endometriosis and 14 women without endometriosis submitted to IVF. Peripheral blood samples were collected before and after administration of human chorionic gonadotropin (hCG), in addition to FF and CM samples. Plasma VEGF levels increased after hCG administration in women with endometriosis and in controls, but were significant only in controls. VEGF levels were lower in FF but were significantly increased in the CM of patients with endometriosis. There was no correlation between VEGF and age, response to ovarian stimulation, oocyte or embryo quality, and pregnancy result. The increase of VEGF levels after hCG in both groups demonstrated a positive effect of this hormone on VEGF. VEGF in the FF and CM presented opposite results in endometriosis, suggesting that granulosa cells may show a different behavior in vivo and in vitro.

show abstract

Section: Discussionmentioning

confidence: 99%

Vascular endothelial growth factor in the plasma, follicular fluid and granulosa cells of women with endometriosis submitted toin vitrofertilization – a pilot study

Oliveira¹,

Abreu²,

Ferriani³

et al. 2005

Gynecological Endocrinology

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show abstract

“…Furthermore, in a previous report [21], we have demonstrated that the concentration of cytokines in follicular fluid does not correlate with the concentration in the supernatant of granulosa cell cultures. We found that the concentrations of VEGF in follicular fluid (FF) and in the supernatant of GC cultures were inversely proportional.…”

Section: Discussionmentioning

confidence: 58%

“…In summary, we conclude that the low response to gonadotropin stimulation as seen in hyporesponders, is likely the result of decreased angiogenesis in the gonad, and specifically in the perifollicular areas, resulting in an increased granulosa cell apoptosis and decreased production of IL-1β and VEGF [17,21]. The concentration of macrophages in hyporesponders is increased within the follicular aspirates, primarily in order to eliminate through phagocytosis the increased apoptotic bodies among the granulosa cells.…”

Section: Discussionmentioning

confidence: 84%

Significance of ovarian macrophages in the follicular aspirates from ART patients

Barañao

Quintana²,

Martín

et al. 2007

J Assist Reprod Genet

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Purpose: To evaluate the percentages of macrophages present in granulosa cells (GC) cultures from patients with different responses to the hyperstimulation, in relation to the percentages of apoptotic cells (ApC), as well as to the release of cytokines.Methods: We studied 42 patients: 12 Hyporesponders, (with ≤ 4 follicles), 15 Normoresponders, (5-14 follicles), and 15 Hyperresponders, ( ≥15 follicles). In GC cultures percentages of macrophages and ApC were counted and, in the conditioned media, cytokines were measuredResults: Percentages of macrophages were significantly higher in GC cultures from Hyporesponders compared with Hyperresponders patients. Also, the percentages of ApC cells were the highest in Hyporesponders. On the contrary, cytokines concentrations were the lowest in this group.Conclusions: The low ovarian response is probably due to the decreased angiogenesis, which in turn produces in-

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“…The apoptosis rate in mural cells has been proposed to be a predictor of ovarian reserves in women undergoing in vitro fertilization (during natural or gonadotropin-stimulated cycles), and high levels of apoptosis were evident in patients with low response, advanced age, and endometriosis (13)(14)(15)(16)(17).…”

mentioning

confidence: 99%

Cumulus cell apoptosis changes with exposure to spermatozoa and pathologies involved in infertility

Diaz‐Fontdevila

Pommer

Smith

2009

Fertility and Sterility

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Relationship of ovarian stimulation response with vascular endothelial growth factor and degree of granulosa cell apoptosis

Cited by 34 publications

References 32 publications

Vascular endothelial growth factor in the plasma, follicular fluid and granulosa cells of women with endometriosis submitted toin vitrofertilization – a pilot study

Vascular endothelial growth factor in the plasma, follicular fluid and granulosa cells of women with endometriosis submitted toin vitrofertilization – a pilot study

Significance of ovarian macrophages in the follicular aspirates from ART patients

Cumulus cell apoptosis changes with exposure to spermatozoa and pathologies involved in infertility

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