Relationship of MDR1 gene polymorphism and P-glycoprotein expression in Chinese refractory lupus nephritis

Abstract

To evaluate the association of multidrug resistance 1 (MDR1) polymorphism and the expression of P-glycoprotein (Pgp) in Chinese refractory lupus nephritis (LN) patients. Polymerase chain reaction-direct sequencing was used to analyze MDR1 polymorphism. The genotype distribution of MDR1 polymorphism in 132 SLE (systemic lupus erythematosus) patients was evaluated. ELISA was used to measure the expression of Pgp. Relationship among Pgp expression, MDR1 polymorphism, SLEDAI (SLE disease activity index), and kidney pathological score was analyzed by using One-way ANOVA and Pearson linear correlation. The frequency distribution of the MDR1 gene was consistent with the Hardy-Weinberg equilibrium. Compared with CT and CC, patients with T/T homozygote in MDR1 C3435T had significantly increased Pgp expression in the refractory group (p < 0.05). Additionally, SLEDAI score was positively correlated with Pgp expression (r = 0.481, p < 0.05). Also, Pgp expression was positively correlated with renal pathological activity index (r = 0.76, p < 0.05). MDR1 C3435T polymorphism is significantly associated with Pgp expression in patients with refractory LN. Pgp expression is closely related to SLEDAI and renal pathological score. Thus, Pgp may be useful in evaluation of the prognosis of patients with refractory LN.

Association between SNPs C1236T and C3435T of the ABCB1 Gene and the Neoadjuvant Chemotherapy in Breast Cancer

“…Various reports on the association between MDR1 gene polymorphisms and chemotherapy response have shown conflicting results. While some researchers have found no association between MDR1 and chemotherapy [22], others have argued the opposite [23].…”

Pharmacogenomics as a Tool in Addressing Genetic VariationDependent Adverse Drug Reactions

“…The presence of homozygous or heterozygous T allele which reduces activity of P-gp predisposed the patients to less elimination of warfarin (Lee and An, 2022). In Chinese Han patients with refractory lupus nephritis, a homozygote T allele was associated with increased P-gp expression, while MDR1 CC and CT genotype with lower P-gp expression (Zhou et al, 2021). In a case of cancer, TT genotype increases the risk of lung cancer, associated with accumulation of carcinogenic xenobiotics (Zawadzka et al, 2020).…”