Relationship of drug resistance to phylogenetic groups of E. coli isolates from wound infections

Saeed, Muhammad; Haque, Abdul; Ali, Aamir; Mohsin, Mashkoor; Bashir, Saira; Tariq, Ayesha; Afzal, Amna; Iftikhar, Tayyaba; Sarwar, Yasra

doi:10.3855/jidc.66

Cited by 8 publications

(8 citation statements)

References 7 publications

(16 reference statements)

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“…In this study, group A had the lowest prevalence. This result is in contrast to those noted in some previous studies of extraintestinal E. coli which indicated that most of isolates belonged to group A [ 15 , 21 , 22 ].…”

Section: Discussioncontrasting

confidence: 99%

Phylogenetic Groups ofEscherichia coliStrains from Patients with Urinary Tract Infection in Iran Based on the New Clermont Phylotyping Method

Iranpour

Hassanpour

Ansari

et al. 2015

BioMed Research International

102

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Objectives. In 2013, Clermont classified E. coli strains into eight phylogenetic groups using a new quadruplex PCR method. The aims of this study were to identify the phylogenetic groups of E. coli based on this method and to assess their antibiotic resistance patterns in Bushehr, Iran. Methods. In this cross-sectional study, 140 E. coli isolates were subjected to phylogenetic typing by a quadruplex PCR method. Antimicrobial susceptibility testing was performed by disk diffusion method. Results. Phylogenetic group B2 was most predominant (39.3%), followed by unknown (27.1%), E (9.3%), C and clade I (each 6.4%), B1 (5%), F and D (each 2.9%), and A (0.7%). The most common antibiotic resistance was related to amoxicillin (82.1%) and the least to meropenem (0.7%). 82.14% of isolates were multiple drug resistant (MDR). Antibiotic resistance was mainly detected in group B2 (50%). Conclusions. Our findings showed the high prevalence of MDR E. coli isolates with dominance of group B2. About 25% of E. coli isolates belong to the newly described phylogroups C, E, F, and clade I. Such studies need to be done also in other regions to provide greater understanding of the antibiotic resistance pattern and the prevalences of different phylogenetic groups.

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Section: Discussioncontrasting

confidence: 99%

Phylogenetic Groups ofEscherichia coliStrains from Patients with Urinary Tract Infection in Iran Based on the New Clermont Phylotyping Method

Iranpour

Hassanpour

Ansari

et al. 2015

BioMed Research International

102

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“…This result was in accordance with other studies [35,36]. The results of two independent studies conducted by Saeed and Dadie indicated that most of the isolates belonged to group A, which is in contrast to our study [37,38]. This difference in the phylogroup distribution can be due to several factors such as genetic, dietary, health, environmental and geographic conditions of the host [39][40][41].…”

Section: Discussionsupporting

confidence: 92%

Determination of the frequency of β-lactamase genes (bla SHV, bla TEM, bla CTX-M) and phylogenetic groups among ESBL-producing uropathogenic Escherichia coli isolated from outpatients

Mirkalantari

Masjedian

Irajian

et al. 2019

Journal of Laboratory Medicine

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Background Escherichia coli accounts for 70–95% of community-acquired urinary tract infections (UTIs). Recently, there has been an increase in the prevalence of extended-spectrum β-lactamase (ESBL) in the community which required an accurate identification for better management. Therefore, the current study was performed to determine the antimicrobial resistance pattern, investigate ESBL phenotypes and genotypes (blaCTX-M, bla TEM and bla SHV genes) and determine the phylogenetic groups among ESBL-positive isolates from outpatients. Methods One hundred and eighty-three positive urine samples were collected from 4450 outpatient clinic attendees. Antibiotic susceptibility was determined and ESBL phenotype screening was carried out using disk diffusion agar and combination disk techniques, respectively. The assessment of the presence of the blaCTX-M, bla TEM and blaSHV genes and phylogenetic grouping were performed using the polymerase chain reaction (PCR) method. Results Out of 183 E. coli isolates, 59 (32.2%) showed a positive ESBL phenotype. The prevalence of ESBL-producing E. coli was higher in males (57.4%). Fifty-seven of the ESBL-producing strains carried at least one of the β-lactamase genes (bla CTX-M, bla TEM, bla SHV). Phylotyping of multi-drug resistant isolates indicated that the isolates belonged to B2, A and D phylogroups. Analysis of resistance patterns among these phylogroups revealed that 74.4%, 55.3% and 29.7% of the isolates in the B2 group were resistant to trimethoprim-sulfamethoxazole, ciprofloxacin and gentamicin, respectively. Most of the strains in the phylogroup B2 carried the bla CTX-M gene. Conclusions All the ESBL-producing isolates were placed in one of the four phylogenetic groups. The presence of CTX-M and resistance to quinolones were more frequent in B2 strains than in non-B2 strains.

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“…In case of E. coli, 39% were found to be MDR, which is in accordance with the previous reports where 33.9% and 34.19% MDR were observed from clinical specimens. 19,20 Prevalence of MDR in K. pneumoniae was found to be 45%, which is much higher than 22.5% reported recently from Karachi. 20 This difference could be due to the low number of K. pneumoniae isolates in both studies.…”

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confidence: 51%

Co-existence of bla _NDM-1 and bla _KPC-2 in clinical isolates of Klebsiella pneumoniae from Pakistan

Sattar

Toleman

Nahid

et al. 2016

Journal of Chemotherapy

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Relationship of drug resistance to phylogenetic groups of E. coli isolates from wound infections

Cited by 8 publications

References 7 publications

Phylogenetic Groups ofEscherichia coliStrains from Patients with Urinary Tract Infection in Iran Based on the New Clermont Phylotyping Method

Phylogenetic Groups ofEscherichia coliStrains from Patients with Urinary Tract Infection in Iran Based on the New Clermont Phylotyping Method

Determination of the frequency of β-lactamase genes (bla SHV, bla TEM, bla CTX-M) and phylogenetic groups among ESBL-producing uropathogenic Escherichia coli isolated from outpatients

Co-existence of bla _NDM-1 and bla _KPC-2 in clinical isolates of Klebsiella pneumoniae from Pakistan

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Relationship of drug resistance to phylogenetic groups of E. coli isolates from wound infections

Cited by 8 publications

References 7 publications

Phylogenetic Groups ofEscherichia coliStrains from Patients with Urinary Tract Infection in Iran Based on the New Clermont Phylotyping Method

Phylogenetic Groups ofEscherichia coliStrains from Patients with Urinary Tract Infection in Iran Based on the New Clermont Phylotyping Method

Determination of the frequency of β-lactamase genes (bla SHV, bla TEM, bla CTX-M) and phylogenetic groups among ESBL-producing uropathogenic Escherichia coli isolated from outpatients

Co-existence of bla NDM-1 and bla KPC-2 in clinical isolates of Klebsiella pneumoniae from Pakistan

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Co-existence of bla _NDM-1 and bla _KPC-2 in clinical isolates of Klebsiella pneumoniae from Pakistan