Relationship of Cell Wall Staining to Gram Differentiation

Bartholomew, J. W.; Finkelstein, Harold

doi:10.1128/jb.75.1.77-84.1958

Cited by 17 publications

(13 citation statements)

References 9 publications

(15 reference statements)

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“…LC-ESI-MS n on permethylated OS-P showed two glycoforms with compositions of Hex1Hep3Kdo and Hex2Hep3Kdo, respectively. MS [2][3][4] showed hexose substitution in both glycoforms on HepI. In the Hex2 glycoform HepII was additionally substituted by one hexose residue.…”

Section: Resultsmentioning

confidence: 97%

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Expression of a new disialyllacto structure in the lipopolysaccharide of non-typeable Haemophilus influenzae

Twelkmeyer

Burström

et al. 2011

Carbohydrate Research

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Section: Resultsmentioning

confidence: 97%

“…Gram-positive bacteria have a thick cell wall that absorbes a dye as discovered by Gram in 1884, while Gram-negative bacteria do not. This is due to the outer membrane that inhibits the inclusion of the dye [2,3]. LPS is essential for growth of Gram-negative bacteria.…”

Section: Introductionmentioning

confidence: 99%

Expression of a new disialyllacto structure in the lipopolysaccharide of non-typeable Haemophilus influenzae

Twelkmeyer

Burström

et al. 2011

Carbohydrate Research

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“…The growth was observed and recorded at the 7th, 14th, 21st and 28th days of incubation. Gram staining was carried out using standard Gram stain method (Bartholomew and Finkelstein, 1958).…”

Section: Morphological and Physiological Characterizationmentioning

confidence: 99%

Antimicrobial activities of Plantactinospora sp. KBS50, a rare marine-derived actinomycete from the coastal area of Sarawak

Juboi¹,

Hwang²,

Yeo³

et al. 2019

MJM

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Aims: An actinomycete strain, designated KBS50, was isolated from a beach sediment sample collected from the Santubong area in Sarawak, Malaysia. This study reports on the identification, characterization and evaluation of the antimicrobial potential of this rare actinomycete. Methodology and results: KBS50 was identified as a potentially new species of Plantactinospora genus using the 16S rRNA gene sequence analysis. The rare actinomycete showed distinct morphological and physiological characteristics from other species of Plantactinospora. KBS50 exhibited strong antagonistic activities against Gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) and fungi (Aspergillus niger, Ganoderma boninense, and Rhizoctonia solani). The actinomycete also tested positive for proteolytic activity. Meanwhile, secondary screening of the cell-free culture broths and the ethyl acetate crude extracts detected antimicrobial activity against the Gram-positive bacteria only. The minimum inhibitory concentration of the crude extract against B. subtilis and S. aureus was 5.21±1.30 µg/mL and 15.63±0.00 µg/mL, respectively. Conclusion, significance and impact of study: The results presented in this paper provided an insight into the capability of Plantactinospora sp. KBS50 as a potential source of bioactive secondary metabolites compounds. This study also showed that the marine-associated environment such as the coastal area in Sarawak can be a valuable source of unique actinomycetes that can be exploited for natural product discovery.

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“…Gram staining method was done according to [22]. For Gram Staining, a single colony from the plate were picked and placed on a slide.…”

Section: Gram Stainingmentioning

confidence: 99%

Isolation and molecular characterization of phytase producing bacteria from Malaysia hot springs

Alias¹,

Shunmugam²,

Ong³

2018

J. Fundam and Appl Sci.

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Potential phytase producing bacterial were isolated from which were Labis (Johor), Dusun Tua (Selangor), Ulu All bacterial samples were grown in Phytase Screening Media 37 °C, 45 C, 50 C and 55 C for four days. Out of all samples tested, five isolates have showed clearing zone surrounding the colony wh were further analyzed in broth culture using sodium phytate as substrate. Among them, strain L3 was selected as the best producer (0.16 U/ml after 72 h of culture). This phytase showed optimal activity at 37 °C and pH5.5, demonstrating appropriate character for application in feed of several industrial processes. Blast homology of 16S rRNA gene sequence of isolate L3 identified 99% similarity to Acinetobacter baumannii.

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Relationship of Cell Wall Staining to Gram Differentiation

Cited by 17 publications

References 9 publications

Expression of a new disialyllacto structure in the lipopolysaccharide of non-typeable Haemophilus influenzae

Expression of a new disialyllacto structure in the lipopolysaccharide of non-typeable Haemophilus influenzae

Antimicrobial activities of Plantactinospora sp. KBS50, a rare marine-derived actinomycete from the coastal area of Sarawak

Isolation and molecular characterization of phytase producing bacteria from Malaysia hot springs

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