Relationship of blood flow to antral lesion incidence in rat gastric mucosa after hemorrhage and retransfusion

Abstract

The relationship of gastric mucosal hemodynamics, energy metabolism, and acid to antral histological lesion incidence following hemorrhage and retransfusion was investigated in rats. Decrease of mucosal blood flow and of the index of mucosal oxygen saturation (ISO2) during hemorrhage were slightly greater in the corpus than in the antrum. ATP content and redox ratio in the corpus were decreased by shock; however, these hardly changed in the antrum. The index of tissue hemoglobin concentration (IHb) recovered to above the prehemorrhage value only in the antrum after retransfusion. Histological lesion incidence in the antral mucosa was more markedly increased by acid instillation and/or retransfusion than in the corpus. From these results, it was concluded that the decrease of blood flow is related as an underlying factor to induction of ischemic damage in the antral mucosa and that intraluminal acid and/or postischemic hyperemia play a causative role in the development of histologic lesions in the antral mucosa during shock and retransfusion in the rat stomach.

Protective Effect of Teprenone on Blood Flow and Incidence of Histologic Lesions in Rat Gastric Mucosa after Hemorrhage and Retransfusion

“…Mucosal tissue samples were collected from both the corpus and antral mucosae, using a local freezing method with the frosted round tip of a glass rod (about 3 mm in diameter) and a thin razor blade as described previously (14). This method is simple, easy, and reproducible, although a small amount of muscle tissue is excised together with mucosal tissue from both the gastric regions.…”

“…This method is simple, easy, and reproducible, although a small amount of muscle tissue is excised together with mucosal tissue from both the gastric regions. The problem in sampling of antral mucosa has been discussed previously (14). Sampling was performed at set time points: before hemorrhage, after 30 and 45min of shock, and 15min after retransfusion.…”

“…4 M perchloric acid solution and was measured bioluminescently, using firefly luciferin-luciferase (Bio-Orbit Oy, Turku, Finland) (14). The protein content in the perchloric acid precipitates was determined by the Lowry method (16).…”

“…Recently, we measured gastric mucosal blood flow and related variables-that is, the index of mucosal oxygen saturation (KO2) and the index of mucosal hemoglobin concentration ( 1HB) -i n a rat hemorrhage-retransfusion model and discussed the relationships among these variables, energy metabolism, and the incidence of histologic lesions in both corpus and antral mucosal regions (14). This model was considered to be more suitable than cold restraint (7) and cirrhosis (8) models to investigate directly the relationship between GMBF and mucosal lesion incidence and the effect of antiulcer drugs on both these variables.…”

“…In the Tween-80 and teprenone groups 2 ml/kg body weight of each solution was additionally administered orally 2 h before blood withdrawal. The procedures used in the following experiments have been described previously in detail (14). All reagents were commercially available reagentgrade compounds.…”