Relationship between viral detection and turbidity in a watershed contaminated with group A rotavirus

Abstract

Enteric viruses are present in aquatic environments due to contamination by raw sewage, even in the absence of fecal coliforms, which are considered to be significant indicators when it comes to microbial water quality assessment. This study investigated the presence of group A rotavirus (RVA) in surface water from a river basin in Minas Gerais, Brazil, assessing the influence of the urbanization, the rainfall, and the microbiological and physico-chemical parameters regarding water quality. Forty-eight surface water samples collected in urbanized and non-urbanized areas, both in dry and rainy periods, were obtained throughout the study. The viral particles were concentrated by adsorption-elution in a negatively charged membrane. Fecal coliforms, as well as physico-chemical water parameters were determined at each point in all collections. The RVA was detected in 62.5 % (30/48) of the water samples through a real-time PCR assay. All the sequenced RVA strains belonged to genotype I1. The RVA was detected in 50.0 % (11/22) of the water samples regarded as being acceptable by current microbiological standards. The presence of the RVA and the viral load were influenced by the collection area (p < 0.05). It was also observed a significant association between the RVA and detecting the turbidity of water (p < 0.05). The collected data showed a high level of contamination in this watershed by the discharge of raw sewage, highlighting the need for urgent measures to improve water quality, ensuring the safe use of this matrix.

Enteric viruses’ dissemination in a private reserve of natural heritage

“…), although other studies with environmental samples in the country showed higher frequency of this virus in rainy seasons (Assis et al . ; Vieira et al . ).…”

“…), it is still one of the most important viral contaminant in environmental samples (Assis et al . ; Elmahdy et al . ; Vieira et al .…”

Prevalence of Rotavirus Genogroup A and Norovirus Genogroup II in Bassaseachic Falls National Park Surface Waters in Chihuahua, Mexico

“…In contrast, Fongaro et al [28] detected up to 65% positive samples in all seasons, which indicated RV prevalence during the year, although this was found to be higher in the winter season. Assis et al [40] detected 54.1% of RV in winter and spring seasons as well as 70.8% during the summer, thus indicating that the transmission of these enteric viruses resulted in higher infection rates through the winter and spring seasons, as stated by the Center for Disease Control and Prevention [41]. Patel et al [42] indicated that transmission patterns, host behavior and susceptibility may contribute to the seasonal prevalence of RV, along with climate conditions.…”

Experimental investigation of suspended particles transport in porous medium under variable temperatures

“…Thus, the traditional oven drying method is unsuitable for our tests, and we need a new way to accurately and rapidly obtain particle concentrations. Previous studies have shown that the turbidity and concentration of particles have a close relationship (Assis et al, 2015). Thus, correlations between turbidity and concentration were studied.…”