Relationship between the state of the surface of four commercial quartz flours and their biological activity in vitro and in vivo

Fubini, Bice; Fenoglio, Ivana; Ceschino, Raffaella; Ghiazza, Mara; Martra, Gianmario; Tomatis, Maura; Borm, Paul J. A.; Schins, Roel P. F.; Bruch, Joachim

doi:10.1078/1438-4639-00277

Cited by 74 publications

(62 citation statements)

References 36 publications

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“…4 In a recent multidisciplinary study [42][43][44][45] on industrial quartz dusts two were nearly inactive and two very active both in cellular toxicity and "in vivo" inflammogenicity. Inactivity was then mainly ascribed to their aluminium content, much higher in the two "less toxic" samples.…”

Section: Cytotoxicity Oxidative Stress and Inflammationmentioning

confidence: 99%

Carbon in Intimate Contact with Quartz Reduces the Biological Activity of Crystalline Silica Dusts

Ghiazza

Tomatis

Doublier

et al. 2012

Chem. Res. Toxicol.

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In order to evaluate the effect of carbonaceous materials on the pathogenic activity of quartz dusts, mixtures of carbon soot (1% and 10%) and quartz (Min-U-Sil ) have been prepared, then milled so to attain an intimate association of carbon and the quartz surface. Both cellular and cell-free tests show that carbon associated to quartz completely inhibits the typical free radical generation of quartz dusts (through Fenton activity and homolytic cleavage of a C-H bond) and suppresses the oxidative stress and inflammation induced by quartz alone on MH-S murine macrophage cells (lipid peroxidation, nitric oxide release, tumour necrosis factor-α synthesis). Cytotoxic response to quartz is also largely reduced. An extremely pure quartz milled with 10% of soot showed inactivating effects on the adverse reactions to quartz similar to Min-U-Sil quartz. None of these effects takes place when the same experiments are carried out with mechanically mixed samples, which suggests that carbon acts not just as a radical quencher, but because of its association to the quartz surface.4

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Section: Cytotoxicity Oxidative Stress and Inflammationmentioning

confidence: 99%

Carbon in Intimate Contact with Quartz Reduces the Biological Activity of Crystalline Silica Dusts

Ghiazza

Tomatis

Doublier

et al. 2012

Chem. Res. Toxicol.

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“…A standard curve was established, and results were expressed in picograms per milliliter of TNF-α. Each experiment was repeated independently three times for each sample and included controls: cells alone (negative control) and DQ12 quartz (toxicological positive control) Fubini et al, 2004).…”

Section: Pro-inflammatory Effectmentioning

confidence: 99%

Adsorption at cell surface and cellular uptake of silica nanoparticles with different surface chemical functionalizations: impact on cytotoxicity

et al. 2014

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International audienceSilica nanoparticles are particularly interesting for medical applications because of the high inertness and chemical stability of silica material. However, at the nanoscale their innocuousness must be carefully verified before clinical use. The aim of this study was to investigate the in vitro biological toxicity of silica nanoparticles depending on their surface chemical functionalization. To that purpose, three kinds of 50 nm fluorescent silica-based nanoparticles were synthesized: 1) sterically stabilized silica nanoparticles coated with neutral polyethylene glycol (PEG) molecules, 2) positively charged silica nanoparticles coated with amine groups and 3) negatively charged silica nanoparticles coated with carboxylic acid groups. RAW 264.7 murine macrophages were incubated for 20 hours with each kind of nanoparticles. Their cellular uptake and adsorption at the cell membrane were assessed by a fluorimetric assay and cellular responses were evaluated in terms of cytotoxicity, pro-inflammatory factor production and oxidative stress. Results showed that the highly positive charged nanoparticle, were the most adsorbed at cell surface and triggered more cytotoxicity than other nanoparticles types. To conclude, this study clearly demonstrated that silica nanoparticles surface functionalization represents a key parameter in their cellular uptake and biological toxicity

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“…34,35 Importantly, the surface properties also play a key role in particle toxicity, as was demonstrated with quartz particles. 33,36,37 For example, increasing the positive surface charge by amino groups directly increases particle uptake 35 and toxicity. 38 Since the cellular interactions and internalization pathways of inhaled particles are going to drive the cellular response and ultimately the impact on the pulmonary system, we investigated the surface-dependent interaction of individual or small aggregates of submicrometer and nanoscale particles, as they are likely to be presented in vivo.…”

mentioning

confidence: 99%

Submicrometer and Nanoscale Inorganic Particles Exploit the Actin Machinery To Be Propelled along Microvilli-like Structures into Alveolar Cells

et al. 2007

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The growing commerce in micro- and nanotechnology is expected to increase human exposure to submicrometer and nanoscale particles, including certain forms of amorphous silica. When inhaled, these particles are likely to reach the alveoli, where alveolar type II epithelial cells that are distinguished by apical microvilli are found. These cells play critical roles in the function of the alveoli and participate in the immune response to amorphous silica and other particles by releasing chemokines. The cellular interactions of the particles, which drive the cellular responses, are still unclear. Adverse effects of nanoparticles have been attributed, in part, to the unique properties of materials at the nanoscale. However, little is known about the cellular interactions of individual or small nanoparticle aggregates, mostly because of their tendency to agglomerate under experimental conditions. Here we investigate the interaction and internalization pathway of individual precipitated amorphous silica particles with specific surface properties and size, by following one particle at a time. We find that both 100 and 500 nm particles can take advantage of the actin turnover machinery within filopodia and microvilli-like structures to advance their way into alveolar type II epithelial cells. This pathway is strictly dependent on the positive surface charge of the particle and on the integrity of the actin filaments, unraveling the coupling of the particle with the intracellular environment across the cell membrane. The retrograde pathway brings a new mechanism by which positive surface charge supports particle recruitment, and potential subsequent toxicity, by polarized epithelial cells bearing microvilli.

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Relationship between the state of the surface of four commercial quartz flours and their biological activity in vitro and in vivo

Cited by 74 publications

References 36 publications

Carbon in Intimate Contact with Quartz Reduces the Biological Activity of Crystalline Silica Dusts

Carbon in Intimate Contact with Quartz Reduces the Biological Activity of Crystalline Silica Dusts

Adsorption at cell surface and cellular uptake of silica nanoparticles with different surface chemical functionalizations: impact on cytotoxicity

Submicrometer and Nanoscale Inorganic Particles Exploit the Actin Machinery To Be Propelled along Microvilli-like Structures into Alveolar Cells

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