Relationship between the role of muscarinic M3 receptors in morphine-induced conditioned place preference and the mesolimbic dopamine system

Li, Xuhong; Zheng, Yuqian; Zhao, Xiaoyan; Cui, Ruisi; Li, Xinwang

doi:10.1016/j.neulet.2022.136774

Cited by 4 publications

(1 citation statement)

References 35 publications

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“…Both cannabinoid CB 1 and acetylcholine M 3 receptors are highly expressed in SH-SY5Y cells used in the present study, which is why they serve as good model for areas within the central nervous system, such as nucleus accumbens and amygdala, known to be linked to addiction [ 28 , 29 , 30 , 31 ]. Here, these receptors also play an important role in processes such as sensory and motor processing, sleep, nociception, mood, stress response, attention, arousal, memory, motivation, and reward [ 32 , 33 ].…”

Section: Discussionmentioning

confidence: 99%

M3 Receptor Pathway Stimulates Rapid Transcription of the CB1 Receptor Activation through Calcium Signalling and the CNR1 Gene Promoter

Marini

Cowie

Ayar

et al. 2023

IJMS

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In this study, we have investigated a possible mechanism that enables CB1/M3 receptor cross-talk, using SH-SY5Y cells as a model system. Our results show that M3 receptor activation initiates signaling that rapidly upregulates the CNR1 gene, resulting in a greatly potentiated CB1 receptor response to agonists. Calcium homeostasis plays an essential intermediary role in this functional CB1/M3 receptor cross-talk. We show that M3 receptor-triggered calcium release greatly increases CB1 receptor expression via both transcriptional and translational activity, by enhancing CNR1 promoter activity. The co-expression of M3 and CB1 receptors in brain areas such as the nucleus accumbens and amygdala support the hypothesis that the altered synaptic plasticity observed after exposure to cannabinoids involves cross-talk with the M3 receptor subtype. In this context, M3 receptors and their interaction with the cannabinoid system at the transcriptional level represent a potential pharmacogenomic target not only for the develop of new drugs for addressing addiction and tolerance. but also to understand the mechanisms underpinning response stratification to cannabinoids.

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