Relationship between the Pathogenicity of the Pine Wood Nematode,<i>Bursaphelenchus xylophilus</i>, and Phenylacetic Acid Production

Kawazu, Kazuyoshi; Zhang, Hong; Yamashita, Hideaki; Kanzaki, Hiroshi

doi:10.1271/bbb.60.1413

Cited by 48 publications

(48 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…There has been no report on the collection of an avirulent PWN isolate since 1984, when OKD1 was isolated (Kawazu et al, 1996); however, some subpopulations had both S and C alleles in the two areas, the region around Kasama City and the southern part of Kujukurihama Coast (Fig. 5).…”

Section: Discussionmentioning

confidence: 99%

Polymorphism of Japanese isolates of the pinewood nematode, Bursaphelenchus xylophilus (Aphelenchida: Aphelenchoididae), at heat-shock protein 70A lucus and the field detection of polymorphic populations

Takemoto

Futai

2007

Appl. Entomol. Zool.

View full text Add to dashboard Cite

Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) pattern of heat-shock protein 70A gene (hsp70A) was examined for 29 isolates of the pinewood nematode. Among them, two known avirulent and five virulent isolates generated banding patterns identical within each of the two isolate groups but distinctive between the two. On the other hand, field populations were collected in two geographical areas, around Kasama City, Ibaraki Prefecture and the southern part of Kujukurihama coast, Chiba Prefecture, Japan. PCR-RFLP image analysis revealed that some of the populations possessed both of the two alleles. The geographical distribution of the alleles was random in the two sampling areas. On the basis of the results, we hypothesize that the avirulent strain might have a certain contribution to the gene pool of the pinewood nematode, although there has been no report on the collection of an avirulent population from Japan recently.

show abstract

Section: Discussionmentioning

confidence: 99%

Polymorphism of Japanese isolates of the pinewood nematode, Bursaphelenchus xylophilus (Aphelenchida: Aphelenchoididae), at heat-shock protein 70A lucus and the field detection of polymorphic populations

Takemoto

Futai

2007

Appl. Entomol. Zool.

View full text Add to dashboard Cite

show abstract

“…375 (e.g. Kawazu et al, 1996;Aikawa et al, 1997Aikawa et al, , 2003Iwahori et al, 1998;Kosaka et al, 2001). Table 1 shows the geographical origin, year established and virulence of each isolate.…”

Section: Methodsmentioning

confidence: 99%

Demonstration of interbreeding between virulent and avirulent populations of Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae) by PCR-RFLP method

Aikawa

Kikuchi

Kosaka

2003

Appl. Entomol. Zool.

View full text Add to dashboard Cite

The pinewood nematode, Bursaphelenchus xylophilus, is the causative agent of pine wilt disease. The virulence of nematodes significantly varies among pine trees, but does not vary within a single pine. To elucidate the reason for no variation in virulence within a single pine, a technique to investigate the population structure of nematodes of different virulence is needed. In this study, the demonstration of interbreeding between virulent and avirulent populations of B. xylophilus in vitro was attempted using PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) method. Ribosomal DNA containing the 5.8S gene, the internal transcribed spacer regions 1 and 2, and partial regions of 18S and 28S genes were used for analysis. First, PCR-RFLP patterns of offspring produced by interbreeding between two individuals of virulent and avirulent isolates were analyzed. The offspring from a single-pair interbreeding showed 3-digested fragment patterns with the restriction enzyme HhaI, patterns the same as the virulent isolate, the avirulent isolate and a hybrid pattern containing both fragments of the virulent and avirulent isolates. The virulent population was mixed with the avirulent one in vitro and propagated nematodes were individually analyzed by PCR-RFLP method. The nematodes showed the same 3 PCR-RFLP patterns as the offspring from a single-pair interbreeding. The detection of nematodes with a hybrid pattern demonstrates the occurrence of interbreeding between virulent and avirulent populations of B. xylophilus.

show abstract

“…The nematode was isolated from a wilted pine tree in the research forest at Okayama University in 1994, and was subcultured on the fungus Botrytis cinerea as described in the previous paper (5 Aseptic Pinus denszflora seedlings inoculated with the aseptic nematode OKD-3 under aseptic conditions survived as well as the control for 16 days after inoculation (Table 1). However, the non-aseptic pine seedlings inoculated with the aseptic nematodes wilted, though a few days later than those inoculated with the non-aseptic nematodes ( Table 2).…”

Section: Methodsmentioning

confidence: 99%

Asepsis of the Pine Wood Nematode Isolate OKD-3 Causes it to Lose its Pathogenicity

Kawazu

Kaneko

1997

Jpn. J. Nematol.

Self Cite

View full text Add to dashboard Cite

Relationship between the Pathogenicity of the Pine Wood Nematode,Bursaphelenchus xylophilus, and Phenylacetic Acid Production

Cited by 48 publications

References 1 publication

Polymorphism of Japanese isolates of the pinewood nematode, Bursaphelenchus xylophilus (Aphelenchida: Aphelenchoididae), at heat-shock protein 70A lucus and the field detection of polymorphic populations

Polymorphism of Japanese isolates of the pinewood nematode, Bursaphelenchus xylophilus (Aphelenchida: Aphelenchoididae), at heat-shock protein 70A lucus and the field detection of polymorphic populations

Demonstration of interbreeding between virulent and avirulent populations of Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae) by PCR-RFLP method

Asepsis of the Pine Wood Nematode Isolate OKD-3 Causes it to Lose its Pathogenicity

Contact Info

Product

Resources

About