Relationship between the nuclear morphology of the sperm of 10 bulls and their fertility

Vieytes, A. L.; Cisale, H.; Ferrari, María Rosa

doi:10.1136/vr.163.21.625

Cited by 8 publications

(12 citation statements)

References 31 publications

(54 reference statements)

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“…2a). We propose that these sperm nuclei were diploid (2n) in agreement descriptions made in other species (Gledhill, 1965;Giuliano et al, 1997;Ferrari et al, 1998;Vieytes et al, 2008). The size of sperm nuclei of the third subpopulation was greater than that of the haploid and diploid nuclei and the intensity of the Feulgen colouration was similar to that of the diploid nuclei (Fig.…”

Section: Discussionsupporting

confidence: 79%

“…The percentage of diploid spermatozoa observed in human semen from fertile men is approximately 0.3% and they originate 8.3% of diandric triploids (Codina-Pascual et al, 2006). Some authors have observed that the shape and size of bovine diploid spermatozoa are very similar to those of normal spermatozoa, and that when they are stained with the Feulgen reaction, diploid spermatozoa are notoriously darker than normal and have a post-acrosomal zone broader than normal (Gledhill, 1965;Ferrari et al, 1998Ferrari et al, , 2006Vieytes et al, 2008). A similar observation has been made in llama diploid spermatozoa (Giuliano et al, 1997).…”

Section: Introductionmentioning

confidence: 92%

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Equine sperm nuclei with different ploidy levels: relationship between the nuclear DNA content and the nuclear area

et al. 2011

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The aims of this study were to estimate the ability of the Feulgen reaction to identify equine sperm nuclei with different ploidy levels, to determine the frequency of haploid, diploid and polyploid sperm nuclei in the semen of fertile equines and to evaluate the relationship between the nuclear DNA content and the nuclear area. Determination of the ploidy level of Feulgen-stained spermatozoa using a scanning microspectrophotometer was very similar to the subjective estimations made with a light microscope. This indicates that the Feulgen reaction is a simple, inexpensive and reliable technique to recognise the ploidy level of equine spermatozoa. The incidence of diploid and polyploid spermatozoa, determined with a light microscope in 11 fertile equines, was 0.17 ± 0.08% and 0.027 ± 0.027% respectively. DNA content values obtained by microspectrophotometry in the only equine that presented polyploid spermatozoa allowed us to discriminate between haploid, diploid and polyploid subpopulations. Measurement of the nuclear area discriminated only two subpopulations: one including the haploid and diploid subpopulations and the other including the polyploid one. The similarity between the area of the haploid and diploid sperm nuclei suggests that the increase in DNA content is anisotropic, with a privileged direction of growth perpendicular to the nuclear flattening plane.

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Section: Discussionsupporting

confidence: 79%

Section: Introductionmentioning

confidence: 92%

Equine sperm nuclei with different ploidy levels: relationship between the nuclear DNA content and the nuclear area

et al. 2011

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“…Thin smears for light microscopy were made from the fixed semen samples and stained with Wright's stain (Rapidiff, Clinical Sciences Diagnostics, Johannesburg, South Africa) before viewing with a 100x oil immersion lens using both bright field and phase contrast microscopy. Smears were also stained with the Feulgen stain and acidic aniline blue according to the methods described by Vieytes et al [30] to evaluate the degree of chromatin condensation. The incidence of the two defects was determined by examining the smears using a 100x oil immersion objective and counting the number 5 of abnormal spermatozoa present in a total of 300 cells for each bird.…”

Section: Methodsmentioning

confidence: 99%

Head-base bending and disjointed spermatozoa in the emu (Dromaius novaehollandiae): A morphological comparison of two closely related defects

Plessis

Soley

2011

Theriogenology

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The accurate assessment of avian sperm abnormalities is hampered by a lack of descriptive data and by the confusing terminology currently in use. Critical appraisal of semen samples from the distal ductus deferens of the emu revealed that two closely related yet separate (distinct) defects previously collectively referred to as "bent sperm" or "crooked-necked sperm" could be identified by light and electron microscopy. Head-base bending typically involved a 180° bend at the base of the nucleus which placed the head and midpiece into close apposition and parallel to each other. No part of the neck or midpiece was involved and bending was restricted exclusively to the base of the nucleus. Incomplete chromatin condensation was always associated with the bend. Disjointed sperm, which superficially resembled "bent" sperm, showed complete separation of the neck from 2 the head-base at the level of the connecting piece. All structural elements of the neck region appeared normal. In both defects the region of contact between the head (nucleus) and the neck/midpiece was enclosed as a unit by the plasmalemma. Both defects were observed to originate in the testis; however, their subsequent expression in the ductus deferens cannot be ruled out. These results confirm that head-base bending of emu sperm represents a head defect, whereas disjointed sperm should be classified as a tail (neck/midpiece) defect.

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“…The majority of studies using the ANBL test have been conducted in humans, suggesting its predictive value for male fertility (Auger et al 1990, Hammadeh et al 2001. Relatively few studies have examined the association of the ANBL test and bull fertility (Vieytes et al 2008). Among the species, the percentage of ANBL-positive sperm cells has been found to be variable, such as 1% in mice (Shokri et al 2013), 10% in cats (Hingst et al 1995), 10% in rabbits (Steger et al 2005), and 50% in roosters (Santiago-Moreno et al 2009).…”

Section: Discussionmentioning

confidence: 99%

Molecular morphology and function of bull spermatozoa linked to histones and associated with fertility

et al. 2013

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Sub-par fertility in bulls is influenced by alterations in sperm chromatin, and it might not be solved with increased sperm concentration in artificial insemination. Appropriate histone retention during sperm chromatin condensation plays critical roles in male fertility. The objective of this study was to determine failures of sperm chromatin condensation associated with abnormal persistence or accessibility of histones by aniline blue (ANBL) test, expression levels, and cellular localizations of one variant and two core histones (H3.3, H2B, and H4 respectively) in the spermatozoa of low-fertility (LF) vs high-fertility (HF) bulls. The expression levels and cellular localizations of histones in spermatozoa were studied using immunoblotting, immunocytochemistry, and staining methods. The bioinformatics focused on the sequence identity and evolutionary distance of these proteins among three mammalian species: bovine, mouse, and human. We demonstrated that ANBL staining was different within the LF (1.73 (0.55, 0.19)) and HF (0.67 (0.17, 0.06)) groups (P!0.0001), which was also negatively correlated with in vivo bull fertility (rZK0.90, P!0.0001). Although these histones were consistently detectable and specifically localized in bull sperm cells, they were not different between the two groups. Except H2B variants, H3.3 and H4 showed 100% identity and were evolutionarily conserved in bulls, mice and humans. The H2B variants were more conserved between bulls and humans, than in mice. In conclusion, we showed that H2B, H3.3, and H4 were detectable in bull spermatozoa and that sperm chromatin condensation status, changed by histone retention, is related to bull fertility.

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Relationship between the nuclear morphology of the sperm of 10 bulls and their fertility

Cited by 8 publications

References 31 publications

Equine sperm nuclei with different ploidy levels: relationship between the nuclear DNA content and the nuclear area

Equine sperm nuclei with different ploidy levels: relationship between the nuclear DNA content and the nuclear area

Head-base bending and disjointed spermatozoa in the emu (Dromaius novaehollandiae): A morphological comparison of two closely related defects

Molecular morphology and function of bull spermatozoa linked to histones and associated with fertility

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