Relationship between the mechanism of hepatitis B virus father–infant transmission and pregnancy outcome

Kong, Ying; Liu, Yi; Liu, Xiaojing; Li, Na; Zhu, Zirong; Zhang, Aiyun; Liu, Jianxue; Ye, Feng; Su, Lin

doi:10.1007/s00404-016-4231-6

Cited by 18 publications

(24 citation statements)

References 16 publications

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“…In this scenario, some studies stated that HBV mRNA was identified in the embryos of HBV-infected fathers, implying the transcription and replication of HBV genes [10,11]. Nevertheless, while previous authors showed significantly lower pregnancy rate (PR) in HBV discordant couples likened to age-matched controls [12], other ones detected higher pregnancy, live birth and implantation rates (IRs) in HBV group [13].…”

Section: Introductionmentioning

confidence: 97%

Hepatitis B Surface Antigen Seropositive Men in Serodiscordant Couples: Effects on the Assisted Reproductive Outcomes

Cito

Coccia

Fucci

et al. 2021

World J Mens Health

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Purpose: To evaluate the influence of hepatitis B virus (HBV) infection in men of serodiscordant couples on the reproductive outcomes. Materials and Methods: A total of 134 infertile couples were included in this retrospective single-center cohort study. Sixty-six couples had hepatitis B surface antigen (HBsAg)-seropositive men and seronegative partners, while 68 couples were controls with both seronegative men and women. Overall, 134 fresh in-vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) treatments were performed. As the main outcome measures, on the day of the fresh IVF/ICSI cycle, we assessed seminal parameters Before and after sperm preparation techniques. Two-pronuclear (2PN) fertilization, 1-2-3PN fertilization, cleavage, miscarriage, pregnancy and live birth rates were collected. Results: No significant differences were found between groups in terms of oocytes retrieved, oocytes injected and embryos obtained (p=0.64, p=0.97, and p=0.40, respectively). The 2PN fertilization rate (FR) was comparable among groups (p=0.51). The 1-2-3PN FR was significantly lower in the HBsAg group than in the control group (66.6% vs. 69.7%, respectively). The clinical pregnancy per cycle, implantation, miscarriage and live birth rate were comparable between the HBsAg group and the control group. The median sperm concentration/ml and total sperm count, measured at baseline and after sperm preparation, was comparable between groups (p>0.05). There was a trend toward significant lower progressive motility (35.0% vs. 55.0%; p<0.05) in the HBsAg group at baseline and after sperm preparation (p<0.05). Conclusions: HBV infected men have the same chance to became father, compared to seronegative patients.

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Section: Introductionmentioning

confidence: 97%

Hepatitis B Surface Antigen Seropositive Men in Serodiscordant Couples: Effects on the Assisted Reproductive Outcomes

Cito

Coccia

Fucci

et al. 2021

World J Mens Health

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“…Sperm with these chromosomal abnormalities can achieve normal fertilization and introduce these aberrations into the embryo, which may increase the risk of abortion, stillbirth, or birth defects [8]. In a recent clinical literature, HBV mRNA was found in abandoned IVF embryos of HBV-infected fathers, which confirmed that HBV could not only enter early cleavage embryos via sperm but also replicate in embryos, resulting in early abortion [47]. The aformentioned findings suggested that HBV may interfere with early embryonic development and thus affect pregnancy outcome.…”

Section: Discussionmentioning

confidence: 93%

Host genes regulate transcription of sperm-introduced hepatitis B virus genes in embryo

Zhong

Liu

Ahmed

et al. 2017

Reproductive Toxicology

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Hepatitis B virus (HBV) can invade the male germline, and sperm-introduced HBV genes could be transcribed in embryo. This study was to explore whether viral gene transcription is regulated by host genes. Embryos were produced by in vitro fertilization of hamster oocytes with human sperm containing the HBV genome. Total RNA extracted from test and control embryos were subjected to SMART-PCR, SSH, microarray hybridization, sequencing and BLAST analysis. Twenty-nine sequences showing significant identity to five human gene families were identified, with CSH2, EIF4G2, PCBD2, PSG4 and TTN selected to represent target genes. Using qRT-PCR, when CSH2 and PCBD2 (or EIF4G2, PSG4 and TTN) were silenced by RNAi, transcriptional levels of HBV s and x genes decreased (or increased). This is the first report that host genes participate in regulation of sperm-introduced HBV gene transcription in embryo, which is critical to prevent negative impact of HBV infection on early embryonic development.

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“…One study showed that when men have an active HBV infection, using testicular sperm aspiration/ percutaneous epididymal sperm aspiration sperm may cause lower fertilization, a high miscarriage rate, and a lower live birth rate (14). Another study reported that IVF embryos of an HBV-positive father were successfully implanted, but early abortion occurred (24). Moreover, although ART dose not increase the risk for mother-to-child transmission of HBV, there is a degree of baseline risk through transplacental, intrapartum, and breastfeeding exposure that ART cannot offset.…”

Section: Discussionmentioning

confidence: 99%

“…Second, the risk for father-to-child transmission of HBV during ART was not investigated in the present study. Previous studies have detected HBV DNA and RNA in embryos from couples with the husband alone being HBsAg-positive, suggesting that the sperm may act as a vector for the vertical transmission of HBV to the embryos, resulting in clinically significant father-to-child HBV transmission (7,24). However, there is evidence that the risk for fatherto-child HBV transmission in natural conception is limited (26).…”

Section: Discussionmentioning

confidence: 99%

Assisted conception does not increase the risk for mother-to-child transmission of hepatitis B virus, compared with natural conception: a prospective cohort study

Nie

Wang

Liao

et al. 2019

Fertility and Sterility

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Objective: To determine whether assisted conception increases the risk for mother-to-child transmission of hepatitis B virus (HBV) infection compared with natural conception. Design: Prospective cohort study. Setting: Research laboratory. Patient(s): A total of 305 children, 176 born with assisted conception and 129 born with natural conception, were born to a total of 251 hepatitis B surface antigen-(HBsAg-) positive women. Intervention(s): None. Main Outcome Measure(s): The seropositive rates of HBsAg in children at birth and HBV infection rates at 9-15 months of age. Result(s): Overall, 7.5% (23/305) of children were HBsAg-positive at birth. The rate of HBsAg-positive children at birth did not significantly differ between children in the assisted conception group compared with those in the natural conception group (6.3% [11/176] vs. 9.3% [12/129]). Multivariate logistic regression analysis showed that conception method is not related to the rate of HBsAg-positive children at birth. All children who were positive for HBsAg at birth and were followed up for 9-15 months became negative for HBsAg after hepatitis B immunization. Conclusion(s): Assisted conception does not increase the risk for mother-to-child transmission of HBV compared with natural conception. (Fertil Steril Ò 2019;111:348-56. Ó2018 by American Society for Reproductive Medicine.) El resumen está disponible en Español al final del artículo.

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Relationship between the mechanism of hepatitis B virus father–infant transmission and pregnancy outcome

Cited by 18 publications

References 16 publications

Hepatitis B Surface Antigen Seropositive Men in Serodiscordant Couples: Effects on the Assisted Reproductive Outcomes

Hepatitis B Surface Antigen Seropositive Men in Serodiscordant Couples: Effects on the Assisted Reproductive Outcomes

Host genes regulate transcription of sperm-introduced hepatitis B virus genes in embryo

Assisted conception does not increase the risk for mother-to-child transmission of hepatitis B virus, compared with natural conception: a prospective cohort study

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