1993
DOI: 10.1136/vr.133.14.341
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Relationship between the levels of antibodies to bovine viral diarrhoea virus in bulk tank milk and the prevalence of cows exposed to the virus

Abstract: A positive relationship was found between the prevalence of cows in a herd which were antibody-positive for bovine viral diarrhoea virus (BVDV) and the level of antibodies to the virus in bulk tank milk as determined by an indirect enzyme-linked immunosorbent assay (ELISA). In herds with an ELISA absorbance value of up to 0.20 there were very few or no antibody-positive cows, whereas in herds with an ELISA absorbance value of at least 0.81, 87 to 100 per cent of the lactating cows were antibody-positive to BVD… Show more

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Cited by 108 publications
(87 citation statements)
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“…Using an indirect ELISA, Niskanen (1993) reported a direct relationship between the prevalence in herds of cows that were positive for the anti-BVDV antibody and antibody titers recorded in BTM. Our results support this idea that antibodies in BTM can be a valuable tool for estimating the prevalence of this viral disease in a herd.…”
Section: Discussionmentioning
confidence: 99%
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“…Using an indirect ELISA, Niskanen (1993) reported a direct relationship between the prevalence in herds of cows that were positive for the anti-BVDV antibody and antibody titers recorded in BTM. Our results support this idea that antibodies in BTM can be a valuable tool for estimating the prevalence of this viral disease in a herd.…”
Section: Discussionmentioning
confidence: 99%
“…The use of this technique to measure antibody titers in the bulk tank milk (BTM) has also been recognized as a valuable tool for this purpose (Niskanen 1993).…”
Section: Introductionmentioning
confidence: 99%
“…O desenvolvimento de um teste ELISA para a detecção de anticorpos anti-BVDV em amostras coletivas de leite (Niskanen et al 1991), aliado à correlação existente entre o nível de anticorpos no leite e a proporção de animais soropositivos nos rebanhos (Niskanen 1993, Beaudeau et al 2001, tem impulsionado programas de vigilância, controle e erradicação da infecção pelo BVDV nos países escandinavos (Paton et al 1998, Lindberg & Alenius 1999. Testes sorológicos em amostras coletadas de recipientes coletivos têm sido utilizados para triagem inicial dos rebanhos.…”
Section: Discus Discus Discus Discus Discussão São São São Sãounclassified
“…Rebanhos que possuem animais PI podem ser identificados por sorologia, já que a presença do vírus resulta em níveis altos de anticorpos na maioria dos animais (Houe 1995). Várias estratégias têm sido utilizadas para a triagem e identificação inicial desses rebanhos: (1) Sorologia de amostras individuais de animais jovens ou de todo o rebanho; (2) Sorologia em amostras coletivas (pool) de soro de animais entre oito e 24 meses de idade; (3) Detecção de anticorpos em amostras individuais de leite; (4) Sorologia em amostras coletivas de leite (Niskanen 1993, Alenius et al 1996, Paton et al 1998. Nesses programas, a utilização de um teste imunoenzimático do tipo ELISA para a pesquisa de anticorpos em amostras coletivas de leite tem viabilizado a realização de triagens de rebanhos em grande escala (Niskanen et al 1991, Paton et al 1998).…”
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