Relationship between the electric conductivity and phosphorus concentration variations in an enhanced biological nutrient removal process

Kim, Kwangsoo; Yoo, Jiseon; Kim, Sung-Youn; Lee, Hee Ja; Ahn, Kwang–Ho; Kim, In Soo

doi:10.2166/wst.2007.053

Cited by 12 publications

(7 citation statements)

References 4 publications

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“…The use of the conductivity sensor was efficient for rapid monitoring of the anaerobic metabolic tests conducted in the off‐line batch cell under standardized medium composition and pH and temperature conditions. As previously explained, conductivity profiles nevertheless comprise the contribution of all ions present in the medium, and might be altered by the evolutions of nitrate in SBRs operated for full BNR (Kim et al, ; Villez et al, ). Further investigations are required toward a possible transposition of this method for the in situ assessment of the active PAO fractions during the anaerobic phase of these SBRs.…”

Section: Discussionmentioning

confidence: 99%

“…including both phosphorus and nitrogen removal (Villez et al, ). Although correlations between conductivity and soluble phosphate concentrations become insignificant in the presence of nitrate in such systems, conductivity has nevertheless been shown to display a direct positive correlation with Pi‐release during strict anaerobic batch phases (Kim et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Multilevel correlations in the biological phosphorus removal process: From bacterial enrichment to conductivity‐based metabolic batch tests and polyphosphatase assays

Weissbrodt

Maillard

Brovelli

et al. 2014

Biotech & Bioengineering

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Enhanced biological phosphorus removal (EBPR) from wastewater relies on the preferential selection of active polyphosphate-accumulating organisms (PAO) in the underlying bacterial community continuum. Efficient management of the bacterial resource requires understanding of population dynamics as well as availability of bioanalytical methods for rapid and regular assessment of relative abundances of active PAOs and their glycogen-accumulating competitors (GAO). A systems approach was adopted here toward the investigation of multilevel correlations from the EBPR bioprocess to the bacterial community, metabolic, and enzymatic levels. Two anaerobic-aerobic sequencing-batch reactors were operated to enrich activated sludge in PAOs and GAOs affiliating with "Candidati Accumulibacter and Competibacter phosphates", respectively. Bacterial selection was optimized by dynamic control of the organic loading rate and the anaerobic contact time. The distinct core bacteriomes mainly comprised populations related to the classes Betaproteobacteria, Cytophagia, and Chloroflexi in the PAO enrichment and of Gammaproteobacteria, Alphaproteobacteria, Acidobacteria, and Sphingobacteria in the GAO enrichment. An anaerobic metabolic batch test based on electrical conductivity evolution and a polyphosphatase enzymatic assay were developed for rapid and low-cost assessment of the active PAO fraction and dephosphatation potential of activated sludge. Linear correlations were obtained between the PAO fraction, biomass specific rate of conductivity increase under anaerobic conditions, and polyphosphate-hydrolyzing activity of PAO/GAO mixtures. The correlations between PAO/GAO ratios, metabolic activities, and conductivity profiles were confirmed by simulations with a mathematical model developed in the aqueous geochemistry software PHREEQC.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Multilevel correlations in the biological phosphorus removal process: From bacterial enrichment to conductivity‐based metabolic batch tests and polyphosphatase assays

Weissbrodt

Maillard

Brovelli

et al. 2014

Biotech & Bioengineering

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“…According to [28] the level of EC is categorized as normal. [29] reported that under anaerobic condition EC has a tendency of showing positive correlation with phosphorus concentrations. The texture of the soil was sand clay.…”

Section: Physico-chemical Properties Of the Soil Used In The Incubation Experimentsmentioning

confidence: 98%

Assessment of Phosphorous Release from Bat Guano with Respect to Their use as Organic Fertilizers in Crop Production

Hatibu¹,

Shitindi²,

Marwa³

2020

IJASRE

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“…Temperature was controlled at 20 ± 1°C. Conductivity in the bulk liquid was used to follow phosphate release and uptake profiles as described in Kim et al (2007). The on-line profiles were used to verify steady operation of the reactor.…”

Section: Reactor Operationmentioning

confidence: 99%

Trehalose as an osmolyte in Candidatus Accumulibacter phosphatis

Graaff

Loosdrecht

Pronk

2020

Appl Microbiol Biotechnol

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Candidatus Accumulibacter phosphatis is an important microorganism for enhanced biological phosphorus removal (EBPR). In a previous study, we found a remarkable flexibility regarding salinity, since this same microorganism could thrive in both freshwater- and seawater-based environments, but the mechanism for the tolerance to saline conditions remained unknown. Here, we identified and described the role of trehalose as an osmolyte in Ca. Accumulibacter phosphatis. A freshwater-adapted culture was exposed to a single batch cycle of hyperosmotic and hypo-osmotic shock, which led to the release of trehalose up to 5.34 mg trehalose/g volatile suspended solids (VSS). Long-term adaptation to 30% seawater-based medium in a sequencing batch reactor (SBR) gave a stable operation with complete anaerobic uptake of acetate and propionate along with phosphate release of 0.73 Pmol/Cmol, and complete aerobic uptake of phosphate. Microbial analysis showed Ca. Accumulibacter phosphatis clade I as the dominant organism in both the freshwater- and seawater-adapted cultures (> 90% presence). Exposure of the seawater-adapted culture to a single batch cycle of hyperosmotic incubation and hypo-osmotic shock led to an increase in trehalose release upon hypo-osmotic shock when higher salinity is used for the hyperosmotic incubation. Maximum trehalose release upon hypo-osmotic shock was achieved after hyperosmotic incubation with 3× salinity increase relative to the salinity in the SBR adaptation reactor, resulting in the release of 11.9 mg trehalose/g VSS. Genome analysis shows the possibility of Ca. Accumulibacter phosphatis to convert glycogen into trehalose by the presence of treX, treY, and treZ genes. Addition of trehalose to the reactor led to its consumption, both during anaerobic and aerobic phases. These results indicate the flexibility of the metabolism of Ca. Accumulibacter phosphatis towards variations in salinity. Key points • Trehalose is identified as an osmolyte in Candidatus Accumulibacter phosphatis. • Ca. Accumulibacter phosphatis can convert glycogen into trehalose. • Ca. Accumulibacter phosphatis clade I is present and active in both seawater and freshwater.

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Relationship between the electric conductivity and phosphorus concentration variations in an enhanced biological nutrient removal process

Cited by 12 publications

References 4 publications

Multilevel correlations in the biological phosphorus removal process: From bacterial enrichment to conductivity‐based metabolic batch tests and polyphosphatase assays

Multilevel correlations in the biological phosphorus removal process: From bacterial enrichment to conductivity‐based metabolic batch tests and polyphosphatase assays

Assessment of Phosphorous Release from Bat Guano with Respect to Their use as Organic Fertilizers in Crop Production

Trehalose as an osmolyte in Candidatus Accumulibacter phosphatis

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