Relationship between the Degree of Cardiovascular Adaptation and Th1/Th2 Polarization of Immune Response

Donnikov, Donnikov A.E.; Shkurnikov, M. Yu.; Акимов, Е. Б.; Tonevitsky, Alexander G.

doi:10.1007/s10517-009-0334-4

Cited by 7 publications

(7 citation statements)

References 9 publications

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“…The results by the authors cited above confirm a diversity of HSPA1A expression depending on the intensity of muscle work, or a physiological character of physical effort. Also Donnikow et al [7] or Sakharov et al [9] obtained similar results. They reported an increase in HSPA1A expression in human white blood cells as a result of short intensive exertion.…”

Section: Discussionmentioning

confidence: 55%

“…thermal stress [1], oxidative stress [2,3], physical effort [4] and many more exogenous or endogenous factors [5,6]. In the recent years, the scientists have studied the effect of physical exertion on the expression of genes including those that encode the heat shock proteins [7,8,9,10]. Antiapoptotic effects of proteins that are encoded by HSPA1A and HSPB1 constitute a protective mechanism of the organism during intensive physical exertion accompanied by severe homeostasis disturbances [5].…”

Section: Introductionmentioning

confidence: 99%

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Are changes in HSPA1A, HSPB1 and LDHb genes expression during physical performance ”till exhaustion” independent of their exercise possibility?

Jastrzębski

Żychowska

2014

Baltic Journal of Health and Physical Activity

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Background: The aim of the study was to designate changes in the expression of HSPA1A, HSPB1 and LDHb in elite rowers after completing a test “till exhaustion” on a rowing ergometer. Finally, we searched for the answer whether there are significant correlations between the expression of the genes and anaerobic threshold (AnT) or the maximal oxygen uptake (VO2max).Material/Methods: The research was conducted on the sample of 9 Polish lightweight male rowers (23.7 ±3.77 yrs, 72.7 ±1.76 kg, 183.6 ±4.58 cm). To determine AnT and VO2max, the subjects performed the test “till exhaustion” with an increasing load on a rowing ergometer. Directly before and after the test, blood samples were collected from the ulnar vein in order to isolate genetic material. RNA was extracted from white cells of venous blood by the chemical method. 2 μg RNA for the reverse transcription was used and the expression of HSPA1A, HSPB1 and LDHb was determined by Real time PCR reaction. To assess the intensity of expression, the ΔΔCt method was used.Results: The study showed an increased expression of HSPA1A and HSPB1 and a decreased one of LDHb. Moreover, post-training changes of the genes activity in white blood cells occurred immediately and could be determined directly after the termination of exertion.Conclusions: No significant correlations between the expression of the genes and anaerobic threshold (AnT), maximal oxygen uptake (VO2max) were stated

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Section: Discussionmentioning

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Are changes in HSPA1A, HSPB1 and LDHb genes expression during physical performance ”till exhaustion” independent of their exercise possibility?

Jastrzębski

Żychowska

2014

Baltic Journal of Health and Physical Activity

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“…It is now clear that measuring changes in the expression of genes associated with the cellular stress response, such as HSPA1A and HSPB1 , synthesized by the cells of an organism in response to a variety of stimuli, including heat, oxidative, metabolic and chemical stress [ 9 , 10 ]may be useful in determining physical workload [ 11 ] and intensity [ 12 – 14 ]or to monitor training [ 15 ]. The literature also suggests that type of physical work [ 16 ] and type of training [ 17 ] may affect the expression of genes encoding heat shock protein (HSP) and interleukins.…”

Section: Introductionmentioning

confidence: 99%

Cellular Stress Response Gene Expression During Upper and Lower Body High Intensity Exercises

et al. 2017

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ObjectivesThe aim was to compare the effect of upper and lower body high-intensity exercise on chosen genes expression in athletes and non-athletes.MethodFourteen elite male artistic gymnasts (EAG) aged 20.6 ± 3.3 years and 14 physically active men (PAM) aged 19.9 ± 1.0 years performed lower and upper body 30 s Wingate Tests. Blood samples were collected before, 5 and 30 minutes after each effort to assess gene expression via PCR.ResultsSignificantly higher mechanical parameters after lower body exercise was observed in both groups, for relative power (8.7 ± 1.2 W/kg in gymnasts, 7.2 ± 1.2 W/kg in controls, p = 0.01) and mean power (6.7 ± 0.7 W/kg in gymnasts, 5.4 ± 0.8 W/kg in controls, p = 0.01). No differences in lower versus upper body gene expression were detected for all tested genes as well as between gymnasts and physical active man. For IL-6 m-RNA time-dependent effect was observed.ConclusionsBecause of no significant differences in expression of genes associated with cellular stress response the similar adaptive effect to exercise may be obtained so by lower and upper body exercise.

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“…The authors of this study are inclined to conclude that excessively long periods of low-intensity training increases the adaptation to the exhaustive efforts so that the same load induces relatively less stress. The varying activity of HSPA1A depending on the intensity of muscle effort and its duration has been reported by such authors as Ryan et al (1991), Donnikov et al (2008), and Sakharov et al (2009). Our studies indicate that different training loads also determine the quantity of transcripts of genes tested.…”

Section: Discussionmentioning

confidence: 67%

Effects of 6-week specific low-intensity training on selected aerobic capacity parameters and HSPA1A, HSPB1, and LDHb gene expression in high-level rowers

Jastrzębski¹,

Żychowska²

2015

Genet. Mol. Res.

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ABSTRACT. The aim of this study was to demonstrate the effects of 6-week low-intensity training on changes in indicators of aerobic capacity and on HSPA1A, HSPB1, and LDHb expression in white blood cells in high level rowers. We hypothesized that the type of training would have an impact not only on the adaptation of athletes to the aerobic nature of the exercises, but also on the expression of genes, designated during exercises "until refusal". Nine Polish lightweight male rowers (21.8 ± 3.77 years of age, 74.2 ± 1.7 6 kg, 184.8 ± 4.58 cm) of high level participated in the experiment. To determine the anaerobic threshold (AnT) and peak oxygen uptake (VO 2max ) at the beginning and end of the 6-week training period, the subjects performed the test "till exhaustion", with increasing load. Directly before and after the test, blood samples were collected from the ulnar vein for isolation of RNA. Consecutively, reverse transcription and real time polymerase chain reaction amplification was performed. A significant influence of applied training on physiological parameters such as VO 2max (P = 0.0001), AnT (W/AT) (P = 0.0007), and maximal acid lactate concentration (P = 0.018) as well as on HSPA1A expression (P = 0.0129) in rowers were detected. The 6-week low-intensity aerobic training significantly affected the physiological parameters and HSPA1A expression in the rowers. Therefore, we suggest that the response of leukocytes by activating HSPA1A was dependent on the type of training. The 6-week period proved sufficiently long to of adapting leukocytes in athletes to high intensity exercises.

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Relationship between the Degree of Cardiovascular Adaptation and Th1/Th2 Polarization of Immune Response

Cited by 7 publications

References 9 publications

Are changes in HSPA1A, HSPB1 and LDHb genes expression during physical performance ”till exhaustion” independent of their exercise possibility?

Are changes in HSPA1A, HSPB1 and LDHb genes expression during physical performance ”till exhaustion” independent of their exercise possibility?

Cellular Stress Response Gene Expression During Upper and Lower Body High Intensity Exercises

Effects of 6-week specific low-intensity training on selected aerobic capacity parameters and HSPA1A, HSPB1, and LDHb gene expression in high-level rowers

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