Relationship between the Contents of Adrenomedullin and Distributions of Neutral Endopeptidase in Blood and Tissues of Spontaneously Hypertensive Rats

Jiang, Hongfeng; Pan, Chun‐Shui; Cai, Daozhang; Qi, Yong-Feng; Pang, Yilin; Tang, Chaoshu

doi:10.1291/hypres.27.109

Cited by 19 publications

(23 citation statements)

References 23 publications

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“…The plasma was separated by centrifugation (1600 g for 15 min, 4 1C) and stored at À70 1C for assaying. 16 Chopped left cardiac ventricles were boiled for 10 min in 1 mol l À1 acetic acid and homogenized with a Polytron at 4 1C. The extract solution was centrifuged at 24 000 g for 30 min.…”

Section: Determination Of Levels Of Ir-imd In Plasma and Cardiac Tissuementioning

confidence: 99%

“…The plasma and tissue extract solution were loaded onto a Sep-Pak C18 cartridge (Phoenix Pharmaceutical) and pre-equilibrated with 0.5 mmol l À1 acetic acid, and the adsorbed material was eluted with 4 ml of 50% CH 3 CN containing 0.1% trifluoroacetic acid. 16 After the samples were lyophilized, the residue was dissolved in radioimmunoassay buffer and assayed according to the manufacturer's instructions. The IC 50 of IMD assay was 614.60 pg ml À1 , and the reactivity with mouse IMD was 100%.…”

Section: Determination Of Levels Of Ir-imd In Plasma and Cardiac Tissuementioning

confidence: 99%

“…The levels of ir-IMD were calculated, and are expressed as pmol l À1 for plasma and pmol per gram protein for tissues. 16 …”

Section: Determination Of Levels Of Ir-imd In Plasma and Cardiac Tissuementioning

confidence: 99%

“…The immunohistochemical localization of IMD was studied according to the method described earlier, 3,16 with some modifications. At 48 h after reperfusion, the hearts were harvested and fixed in Carnoy's fluid, then cut into three sections corresponding approximately to the apex, mid-papillary and base.…”

Section: Imd Immunohistochemistrymentioning

confidence: 99%

“…16 Total tissue RNAs of the control mice and ischemic mice 3 and 48 h after reperfusion (three in each group) were extracted and reverse transcribed. Subsequently, the products were used for PCR amplification in 25 ml of a PCR reaction mixture containing 1 ml 2.5 mmol l À1 dNTP, 2.5 ml 10ÂPCR buffer (20 mmol l À1 MgCl2, 500 mmol l À1 KCl, 1.5 mol l À1 Tris-HCl, pH 8.7), 2 ml cDNA, 200 nmol l À1 of the appropriate paired primers ( (Table 1), followed by 74 1C for 5 min.…”

Section: Reverse Transcriptase-pcr Studymentioning

confidence: 99%

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Intermedin is upregulated and has protective roles in a mouse ischemia/reperfusion model

et al. 2009

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Intermedin (IMD), a new calcitonin/calcitonin gene-related peptide family peptide with vasodilatory and positive inotropic properties, has multiple functions in regulating cardiovascular homeostasis and is of particular interest in the pathophysiology of myocardial ischemia/reperfusion (MI/R). We created a mouse model of MI/R by ligating the cardiac left anterior descending artery to study the possible pathophysiological role of IMD and its receptor complexes in MI/R. Compared with the control, infarcted mice showed increased content, mRNA and protein expression of IMD in plasma and cardiac tissue. The mRNA expression of the receptor activity-modifying protein 3 (RAMP3) gene increased very early, and the calcitonin receptor-like receptor and RAMP2 mRNA levels increased later after reperfusion. However, the RAMP1 gene expression did not change. The tissue IMD content was positively correlated with the diastolic blood pressure and negatively correlated with pulse pressure. In addition, exogenous IMD treatment significantly ameliorated the MI/R injury by rescuing the pulse pressure, inhibiting neutrophil infiltration in the peri-infarction area, and decreasing the creatine kinase and lactate dehydrogenase activities in plasma. Our results indicated that IMD was upregulated in the ischemic myocardium and may induce important beneficial cytoprotection against cardiac ischemic injury.

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Section: Determination Of Levels Of Ir-imd In Plasma and Cardiac Tissuementioning

confidence: 99%

Section: Determination Of Levels Of Ir-imd In Plasma and Cardiac Tissuementioning

confidence: 99%

“…The levels of ir-IMD were calculated, and are expressed as pmol l À1 for plasma and pmol per gram protein for tissues. 16 …”

Section: Determination Of Levels Of Ir-imd In Plasma and Cardiac Tissuementioning

confidence: 99%

Section: Imd Immunohistochemistrymentioning

confidence: 99%

Section: Reverse Transcriptase-pcr Studymentioning

confidence: 99%

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Intermedin is upregulated and has protective roles in a mouse ischemia/reperfusion model

et al. 2009

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Effect of Valsartan on Cerebellar Adrenomedullin System Dysregulation During Hypertension

Figueira

Israel

2016

Cerebellum

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Adrenomedullin (AM) and its receptors components, calcitonin-receptor-like receptor (CRLR), and receptor activity-modifying protein (RAMP1, RAMP2, and RAMP3) are expressed in cerebellum. Cerebellar AM, AM binding sites and receptor components are altered during hypertension, suggesting a role for cerebellar AM in blood pressure regulation. Thus, we assessed the effect of valsartan, on AM and its receptor components expression in the cerebellar vermis of Wistar Kyoto (WKY) and spontaneously hypertensive (SHR) rats. Additionally, we evaluated AM action on superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activity, and thiobarbituric acid reactive substances (TBARS) production in cerebellar vermis. Animals were treated with valsartan or vehicle for 11 days. Rats were sacrificed by decapitation; cerebellar vermis was dissected; and AM, CRLR, RAMP1, RAMP2, and RAMP3 expression was quantified by Western blot analysis. CAT, SOD, and GPx activity was determined spectrophotometrically and blood pressure by non-invasive plethysmography. We demonstrate that AM and RAMP2 expression was lower in cerebellum of SHR rats, while CRLR, RAMP1, and RAMP3 expression was higher than those of WKY rats. AM reduced cerebellar CAT, SOD, GPx activities, and TBARS production in WKY rats, but not in SHR rats. Valsartan reduced blood pressure and reversed the altered expression of AM and its receptors components, as well the loss of AM capacity to reduce antioxidant enzyme activity and TBARS production in SHR rats. These findings demonstrate that valsartan is able to reverse the dysregulation of cerebellar adrenomedullinergic system; and they suggest that altered AM system in the cerebellum could represent the primary abnormality leading to hypertension.

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Chronic Effect of Combined Treatment With Omapatrilat and Adrenomedullin on the Progression of Heart Failure in Rats

Nishikimi

Mori

Ishimura

et al. 2006

American Journal of Hypertension

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Relationship between the Contents of Adrenomedullin and Distributions of Neutral Endopeptidase in Blood and Tissues of Spontaneously Hypertensive Rats

Cited by 19 publications

References 23 publications

Intermedin is upregulated and has protective roles in a mouse ischemia/reperfusion model

Intermedin is upregulated and has protective roles in a mouse ischemia/reperfusion model

Effect of Valsartan on Cerebellar Adrenomedullin System Dysregulation During Hypertension

Chronic Effect of Combined Treatment With Omapatrilat and Adrenomedullin on the Progression of Heart Failure in Rats

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