Relationship between the carbohydrate content of apoproteins of vldl, idl and ldl and the plasma level of these lipoproteins in man

Fontaine, M; Malmendier, Claude

doi:10.1016/0021-9150(78)90121-1

Cited by 4 publications

(2 citation statements)

References 18 publications

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“…Other authors have also reported lower contents of N -acetyl glucosamine and mannose in comparison with the expected levels. 16 , 17 In their calculations, Taniguchi et al 22 were based on the expectation that 13–16 of asparagine residues in apo-B are glycosylated. The available data support the suggestion that not all these asparagine residues are glycosylated.…”

Section: Discussionmentioning

confidence: 99%

Carbohydrate composition of circulating multiple-modified low-density lipoprotein

Zakiev

Sobenin

Sukhorukov

et al. 2016

VHRM

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Atherogenic modification of low-density lipoprotein (LDL) plays a crucial role in the pathogenesis of atherosclerosis, as modified LDL, but not native LDL, induces pronounced accumulation of cholesterol and lipids in the arterial wall. It is likely that LDL particles undergo multiple modifications in human plasma: desialylation, changes in size and density, acquisition of negative electric charge, oxidation, and complex formation. In a total LDL preparation isolated from pooled plasma of patients with coronary atherosclerosis and from healthy subjects, two subfractions of LDL could be identified: desialylated LDL bound by a lectin affinity column and normally sialylated (native) LDL that passed through the column. The desialylated LDL subfraction therefore represents circulating modified LDL. In this work, we performed a careful analysis of LDL particles to reveal changes in the composition of glycoconjugates associated with proteins and lipids. Protein fraction of LDL from atherosclerotic patients contained similar amounts of glucosamine, galactose, and mannose, but a 1.6-fold lower level of sialic acid as compared to healthy donors. Lipid-bound glycoconjugates of total LDL from patients with coronary atherosclerosis contained 1.5–2-fold less neutral monosaccharides than total LDL from healthy donors. Patient-derived LDL also contained significantly less sialic acid. Our results demonstrate that carbohydrate composition of LDL from atherosclerotic patients was altered in comparison to healthy controls. In particular, prominent decrease in the sialic acid content was observed. This strengthens the hypothesis of multiple modification of LDL particles in the bloodstream and underscores the clinical importance of desialylated LDL as a possible marker of atherosclerosis progression.

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Section: Discussionmentioning

confidence: 99%

Carbohydrate composition of circulating multiple-modified low-density lipoprotein

Zakiev

Sobenin

Sukhorukov

et al. 2016

VHRM

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“…The validity of correction introduced into standard Warren's assay was assessed in our recent work, where carbohydrate content of LDL was determined also by anion exchange chromatography using the pulsed amperometric detection method, and practically coinciding estimates have been obtained (16). Moreover, we have compared the data on LDL sialic acid content obtained in different studies (Table 2) (2,3,6,7,13,16,(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35). For convenience, the values are expressed as mol sialic acid per mol LDL protein.…”

Section: Discussionmentioning

confidence: 99%

Optimization of the assay for sialic acid determination in low density lipoprotein

Собенин

Tertov

Orekhov

1998

Journal of Lipid Research

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Sialic acid level in blood plasma and circulating glycoproteins is considered to be a marker for a number of pathologic conditions, including atherosclerosis, cancer, etc. The precise measurement of sialic acid level is an important laboratory procedure to allow correct interpretation of results. Colorimetric methods commonly used for the measurement of sialic acid are not highly specific, as interfering substances may alter the results. Among these, malondialdehyde and other aldehydes play the decisive role. In the circulation, aldehydes are commonly produced during lipid peroxidation in the lipid core of lipoprotein particles, especially low density lipoprotein (LDL). To establish the impairment to the sialic acid determination in LDL introduced by interfering substances, the optimized assay based on Warren's traditional method was developed and tested in 606 LDL samples. The optimization implies the comparison of color developed using the standard Warren procedure with that due to contaminating agents, mainly thiobarbituric acid-reactive substances (TBARS). In LDL stored at 4 ؇ C, the estimates obtained by the modified procedure were 41.5% or 30.1 nmol/mg lower, on average, compared to the standard procedure (n ‫؍‬ 45, P Ͻ 0.0001). Even in LDL stored at ؊ 70 ؇ C, sialic acid estimates obtained by the modified procedure were 6.6% or 3.6 nmol/mg lower, on average, compared to the standard measurement (n ‫؍‬ 561, P Ͻ 0.005). Thus, the modified procedure avoids significant distortion of the measurement induced by the presence of interfering agents.-Sobenin, I. A., V. V. Tertov, and A. N. Orekhov. Optimization of the assay for sialic acid determination in low density lipoprotein.

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The effect of fasting on the interactions between human low density lipoproteins and calcium, heparin and chondroitin sulfate

Fontaine¹,

Malmendier²

1979

Clinica Chimica Acta

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Relationship between the carbohydrate content of apoproteins of vldl, idl and ldl and the plasma level of these lipoproteins in man

Cited by 4 publications

References 18 publications

Carbohydrate composition of circulating multiple-modified low-density lipoprotein

Carbohydrate composition of circulating multiple-modified low-density lipoprotein

Optimization of the assay for sialic acid determination in low density lipoprotein

The effect of fasting on the interactions between human low density lipoproteins and calcium, heparin and chondroitin sulfate

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