Relationship between susceptibility to antimicrobials and virulence factors in paediatric Escherichia coli isolates

Arısoy, Münevver; Rad, Abbas Yousefi; Akin, Ali R.; Akar, Nejat

doi:10.1016/j.ijantimicag.2007.07.030

Cited by 20 publications

(30 citation statements)

References 15 publications

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“…Also, our study, similar to Velasco et al, showed that the resistance to nalidixic acid caused loss of virulence genes; therefore, resistance to nalidixic acid may lead to the loss of the ability to pathogenicity (17). In a study done by Arisoy et al, they showed an association between susceptibility to antibiotics and virulence factors in the E. coli isolates recovered from UTI patients (18). Similar to the present study, they reported that virulence gene hlyA was increased in sensitive isolates.…”

supporting

confidence: 84%

Correlation Between hlyA and cnf1 Virulent Genes with Antibiotic Resistance and non-ESBLs Escherichia coli Isolates Collected from Patient with Urinary Tract Infections in Kerman, Iran

Hashemizadeh

Kalantar-Neyestanaki

Mansouri

2017

Arch Pediatr Infect Dis

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Background: Escherichia coli is the most common cause of urinary tract infections. Different studies have been reported that acquisition of drug resistance mechanism may be related to loss of virulent factors. In this study, we investigated the correlation of antibiotic resistance and ESBLs production with presence of hlyA and cnf1 virulence genes. Methods: During June 2014 to March 2015, 250 Escherichia coli isolates were collected from different patients with urinary tract infections in Kerman, Iran. Disk diffusion method was used for determination of antibiotic susceptibility profile and combined disk method was used for detection of ESBLs producing isolates. The hlyA and cnf1 genes were detected by PCR method. Results: In total, 44.8% of isolates were considered as extended spectrum beta-lactamases (ESBLs) producing by combined disk method. hlyA and cnf1 genes were detected in 28.8% and 29.2% of isolates, respectively. Statistical analysis revealed significant correlations between susceptibility to NA, CIP, CTX, CAZ, PIT antibiotics with hlyA and cnf1 positive isolates and also significant correlation observed between ESBLs negative isolates with hlyA positive isolates (P value < 0.05). Conclusions: Acquire of antibiotic resistance mechanism may lead to loss of virulence factor.

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supporting

confidence: 84%

Correlation Between hlyA and cnf1 Virulent Genes with Antibiotic Resistance and non-ESBLs Escherichia coli Isolates Collected from Patient with Urinary Tract Infections in Kerman, Iran

Hashemizadeh

Kalantar-Neyestanaki

Mansouri

2017

Arch Pediatr Infect Dis

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“…This work was motivated by the fact that antibiotic resistance and attachment factors can be encoded together on mobile genetic elements (23), leading to the proposition of a significant relationship between the attachment phenotype and antibiotic resistance. This relationship has previously been observed with clinical isolates (2,24,25); the work presented here is the first investigation of agricultural isolates. We found that resistance to 6 of the 13 tested antibiotics is significantly and positively associated with quartz attachment (Fig.…”

Section: Discussionsupporting

confidence: 79%

“…For example, Liverell's study showed that antibiotic-resistant bacteria have increased adherence to human intestinal cells relative to antibiotic-sensitive strains (28). Ampicillin resistance has been associated specifically with P pili and Dr-type attachment factors (2,24,25), and tobramycin resistance has been associated with afimbrial adhesion, S fimbriae, and P pili (2). This relationship is not surprising when one considers that mobile genetic elements carrying both attachment and resistance-associated genes have been found in E. coli (29,39).…”

mentioning

confidence: 99%

Association of Antibiotic Resistance in Agricultural Escherichia coli Isolates with Attachment to Quartz

Liu

Soupir

Zwonitzer

et al. 2011

Appl Environ Microbiol

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Surface water can be contaminated by bacteria from various sources, including manure from agricultural facilities. Attachment of these bacteria to soil and organic particles contributes to their transport through the environment, though the mechanism of attachment is unknown. As bacterial attachment to human tissues is known to be correlated with antibiotic resistance, we have investigated here the relationship between bacterial attachment to environmental particles and antibiotic resistance in agricultural isolates. We evaluated 203 Escherichia coli isolates collected from swine facilities for attachment to quartz, resistance to 13 antibiotics, and the presence of genes encoding 13 attachment factors. The genes encoding type I, EcpA, P pili, and Ag43 were detected, though none was significantly related to attachment. Quartz attachment was positively and significantly (P < 0.0038) related to combined resistance to amoxicillin/streptomycin/tetracycline/sulfamethazine/ tylosin/chlortetracycline and negatively and significantly (P < 0.0038) related to combined resistance to nalidixic acid/kanamycin/neomycin. These results provide clear evidence for a link between antibiotic resistance and attachment to quartz in agricultural isolates. We propose that this may be due to encoding by the responsible genes on a mobile genetic element. Further exploration of the relationship between antibiotic resistance and attachment to environmental particles will improve the understanding and modeling of environmental transport processes, with the goal of preventing human exposure to antibiotic-resistant or virulent microorganisms.

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“…Although some investigators suggested that virulence genes increased antibiotic resistance of previously resistant strains (16), others observed that quinoloneresistant E. coli strains were more prone to induce Cys rather than PN (17,18,20) because of decreased renal invasive capacity concomitantly acquired by the mutation. Our findings, in agreement with those reports, showed a lower proportion of E. coli resistance to first-generation quinolones in PN isolates: the risk of resistance in PN isolates to nalidixic acid was reduced in the whole sample (OR ϭ 2.90) and to pipemidic acid in the Tx group (OR ϭ 2.57).…”

Section: Discussionmentioning

confidence: 99%

“…The acquisition of antibiotic resistance may be associated with phenotypic changes in bacteria that render them more virulent (16). On the other hand, other investigators have suggested that quinolone resistance involves a genotypic change associated with the loss of virulence factors in E. coli (17)(18)(19)(20).…”

mentioning

confidence: 99%

The Role of Host Factors and Bacterial Virulence Genes in the Development of Pyelonephritis Caused by Escherichia coli in Renal Transplant Recipients

Siliano

Rocha

Medina-Pestana

et al. 2010

Clinical Journal of the American Society of Nephrology

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Background and objectives: The aim of this study was to determine the role of host factors and bacterial virulence genes in the development of pyelonephritis caused by Escherichia coli in renal transplant (Tx) recipients.Design, setting, participants, & measurements: A total of 328 E. coli isolates from cases of cystitis (Cys; n ‫؍‬ 239) or pyelonephritis (PN; n ‫؍‬ 89), with 169 from renal Tx recipients, were subjected to molecular analyses to identify P-fimbria subunits (PapC, PapG II, and PapGIII), G-and M-fimbriae, and aerobactin. The presence of antibiotic resistance was also determined. Parameters such as gender, age, immunosuppression regimens, causes of ESRD, kidney donor, intraoperative anastomosis, use of double J stent, trimethoprim/sulfamethoxazole (TMP/SMZ) prophylaxis, and time after Tx were evaluated.Results: A multivariate analysis showed a significant association between PN and renal Tx. In renal Tx recipients, the risk of occurrence of PN was significantly higher among males and for those no longer receiving TMP/SMZ prophylaxis. E. coli strains isolated from PN presented a lower prevalence of papGIII and lower rates of resistance to pipemidic acid. Although papGII was more prevalent in PN than in Cys, it was not independently associated with PN.Conclusions: These findings suggested that renal Tx increases the risk for PN, and the male sex represented a host factor independently associated with risk, whereas the prophylaxis with TMP/SMZ was protective. The lack of papGIII and low resistance to first-generation quinolones were bacterial-independent risk factors for PN in Tx.

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Relationship between susceptibility to antimicrobials and virulence factors in paediatric Escherichia coli isolates

Cited by 20 publications

References 15 publications

Correlation Between hlyA and cnf1 Virulent Genes with Antibiotic Resistance and non-ESBLs Escherichia coli Isolates Collected from Patient with Urinary Tract Infections in Kerman, Iran

Correlation Between hlyA and cnf1 Virulent Genes with Antibiotic Resistance and non-ESBLs Escherichia coli Isolates Collected from Patient with Urinary Tract Infections in Kerman, Iran

Association of Antibiotic Resistance in Agricultural Escherichia coli Isolates with Attachment to Quartz

The Role of Host Factors and Bacterial Virulence Genes in the Development of Pyelonephritis Caused by Escherichia coli in Renal Transplant Recipients

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