Relationship between substances in seminal plasma and Acrobeads Test∗ results

Komori, Kazuhiko; Tsujimura, Akira; Okamoto, Yasuaki; Matsuoka, Yoshikazu; Takao, Tetsuya; Miyagawa, Yasushi; Takada, Shingo; Nonomura, Norio; Okuyama, Äkïhïko

doi:10.1016/j.fertnstert.2007.11.003

Cited by 5 publications

(6 citation statements)

References 41 publications

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“…In our previous report, the Acrobeads score was found to be related to the sperm concentration and sperm motility in 81 ejaculates [27]. Komori et al reported that sperm motility and the percentage of sperm with an abnormal morphology had an effect on the Acrobeads test results in 114 ejaculates [33]. In the present study, a similar trend was observed; however, it was not statistically significant.…”

Section: Discussionsupporting

confidence: 80%

“…In this context, the Acrobeads test can be used to evaluate acrosome reactions in the sperm using a monoclonal antibody that binds to the anterior portion of acrosome-reacted sperm [23, 24]. The results of tests using Acrobeads show good reproducibility and are correlated with the results of sperm penetration assays using zona-free hamster eggs and IVF [24, 33, 34]. Therefore, the acrosome status determined according to the Acrobeads test is a valuable parameter for estimating the capacity for fertilization in males with infertility.…”

Section: Discussionmentioning

confidence: 99%

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Sperm Nuclear Vacuoles in relation to Acrosome Reactions and Sperm Motility

Komiya

Kawauchi

Kato

et al. 2014

The Scientific World Journal

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We investigated sperm nuclear vacuolation in relation to acrosome reactions and the maintenance of sperm motility. Thirty male patients who visited our Male Infertility Clinic were enrolled. These patients underwent conventional semen analyses, Acrobeads tests, and high-magnification observation of the sperm head to evaluate the degree of nuclear vacuolation on the Acrobeads test scoring after 24 hours of incubation. The presence of acrosome reactions was evaluated using the Acrobeads test. The spermatozoa were classified into three groups: (I) those bound to MH61-beads, (II) motile spermatozoa that did not bind to MH61-beads, and (III) immotile spermatozoa that did not bind to MH61-beads. The percentage of spermatozoa with large nuclear vacuoles (%LNV) was compared between the three groups. The degree of sperm nuclear vacuolation was evaluated in 17,992 ejaculated spermatozoa. The mean %LNVs were 2.4% in group I, 5.8% in group II, and 9.8% in group III. These values were significantly different from each other (P < 0.001, paired t-test). There were no correlations between the %LNV values and the Acrobeads scores. In conclusion, the degree of sperm nuclear vacuolation was significantly lower in the acrosome-reacted spermatozoa and spermatozoa with maintained motility, and higher in the immotile spermatozoa that did not bind to MH61-beads.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Sperm Nuclear Vacuoles in relation to Acrosome Reactions and Sperm Motility

Komiya

Kawauchi

Kato

et al. 2014

The Scientific World Journal

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“…DFI is a clinically useful marker of male infertility. 48 Komori et al 49 reported that MIF in seminal plasma was significantly higher in patients with abnormal acrosome reactions. These results are in agreement with previous findings.…”

Section: Discussionmentioning

confidence: 99%

Effect of scrotal heating on sperm quality, seminal biochemical substances, and reproductive hormones in human fertile men

Zhang

Zhai

Fang

et al. 2018

J of Cellular Biochemistry

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At present, male contraceptive methods are only vasectomy and condoms, so it is necessary to research on male contraceptive techniques. The aim of this study is to observe the effects of scrotal heating (SH) on semen parameters, seminal l-carnitine (LC), epidermal growth factor (EGF), macrophage migration inhibitory factor (MIF), reproductive hormones and sperm chromosome numbers of adult healthy men, and to provide the experimental data for male contraception. The scrotums of 30 healthy male volunteers were exposed to the condition of 40 to 43°C SH belt warming 40 minutes each day for successive 2 days per week. The course of SH was continuous for 3 months. Computer-assisted semen analysis and hypo-osmotic swelling test, sperm DNA integrity, l-carnitine, MIF and EGF, and sperm fluorescence in situ hybridization were performed before, during, and after SH. The serum level of follicle stimulating hormone (FSH), luteinizing hormone (LH), and testosterone (T) were measured by chemiluminescent immunoassay. The mean parameters of sperm concentration, vitality, and normal morphological sperm were significantly decreased in groups with sperms being collected during 1, 2, and 3 months of SH when compared with those in groups of pre-SH (P < 0.01). Statistically significant differences of sperm DNA fragmentation, normal sperm membrane functionality, levels of LC and MIF in semen, and LH, FSH, and T in serum were observed between the groups of before SH and after SH 3 months and the groups of during SH 1, 2, and 3 months (P < 0.001). The total rate of chromosome number for 13, 18, 21, X, and Y in the 3 months of SH was 13.7-fold greater (13.72%/1.69%) than before SH (P < 0.001). The constant SH can impact the semen quality, sperm DNA integrity, sperm chromosome, LC and MIF, and LH, FSH, and T in serum. Transient SH may be a new method for male contraception.

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“…An analysis of semen from infertile men has shown that 14.4% of anomalous patriarchal methylation occurred in H19 and gene trap locus 2 gene (93)(94)(95). In spermatozoa from oligozoospermic men, the occurrence of hypermethylation at several maternal DMRs or hypomethylation of H19 and intergenic-DMR was increased, particularly in patients with ejaculation volumes of <10x10 6 /ml (96)(97)(98)(99).…”

Section: Dna Methylation and Male Infertilitymentioning

confidence: 99%

DNA methylation in spermatogenesis and male infertility

Cui¹,

Xuan

Wu³

et al. 2016

Experimental and Therapeutic Medicine

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Abstract. Infertility is a significant problem for human reproduction, with males and females equally affected. However, the molecular mechanisms underlying male infertility remain unclear. Spermatogenesis is a highly complex process involving mitotic cell division, meiosis cell division and spermiogenesis; during this period, unique and extensive chromatin and epigenetic modifications occur to bring about specific epigenetic profiles in spermatozoa. It has recently been suggested that the dysregulation of epigenetic modifications, in particular the methylation of sperm genomic DNA, may serve an important role in the development of numerous diseases. The present study is a comprehensive review on the topic of male infertility, aiming to elucidate the association between sperm genomic DNA methylation and poor semen quality in male infertility. In addition, the current status of the genetic and epigenetic determinants of spermatogenesis in humans is discussed. Contents1. Introduction 2. DNA methylation 3. DNA methylation and spermatogenesis 4. DNA methylation and genomic imprinting 5. DNA methylation and male infertility 6. Conclusion IntroductionEpigenetics is the study of genomic structural modifications that affect gene expression without altering the underlying nucleotide sequence (1-3). Epigenetic mechanisms involved in the regulation of gene expression include the regulation of non-coding RNA, chromatin remodeling, DNA methylation and histone modifications (4,5). Of these mechanisms, DNA methylation has been implicated in numerous biological functions, such as the development of spermatozoa and early embryos, and the repression of endogenous retrotransposons, while it also has a wide range of effects in gene expression (2,3,6). The dysregulation of DNA methylation has previously been associated with various human disorders, and has been shown to increase the risk of fertilization failure, dysfunction in embryogenesis, perinatal mortality, congenital abnormalities, preterm birth and low birth weight (7-11). The present review assesses the significance of DNA methylation in spermatogenesis in order to elucidate the association between the dysregulation of DNA methylation and male infertility. This may provide a basis for the prevention and treatment of male infertility, as well as permit the evaluation of the epigenetic quality of sperm in order to reduce the risk of epigenetic diseases in cases where conception is performed by assisted reproductive technology (ART). DNA methylationEpigenetic mechanisms are critical regulators of gene expression during spermatogenesis that may influence male fertility (12-14). Cytosine, a key DNA base, is methylated at the position, typically in the context of CpG dinucleotides. The methylation of constitutive heterochromatic and promoter regions is generally associated with reduced gene transcription (Fig. 1A) (15-18). Therefore, DNA methylation is a type of epigenetic modification that can effectively promote gene silencing (Fig. 1B). The methyl group for this chemical modif...

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Relationship between substances in seminal plasma and Acrobeads Test∗ results

Cited by 5 publications

References 41 publications

Sperm Nuclear Vacuoles in relation to Acrosome Reactions and Sperm Motility

Sperm Nuclear Vacuoles in relation to Acrosome Reactions and Sperm Motility

Effect of scrotal heating on sperm quality, seminal biochemical substances, and reproductive hormones in human fertile men

DNA methylation in spermatogenesis and male infertility

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