Relationship between sperm telomere length and sperm quality in infertile men

Darmishonnejad, Zahra; Zarei-Kheirabadi, Farzaneh; Tavalaee, Marziyeh; Zarei-Kheirabadi, Masoumeh; Zohrabi, Dina; Nasr-Esfahani, Mohammad Hossein

doi:10.1111/and.13546

Cited by 38 publications

(51 citation statements)

References 43 publications

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“…This study used TUNEL and CMA3 staining to assess DNA fragmentation and protamine deficiency in sperm cells respectively. In line with other studies (Darmishonnejad et al., 2020; Rocca et al., 2016; Tahamtan et al., 2019), our finding showed a high level of DNA fragmentation and protamine deficiency in men with oligozoospermia.…”

Section: Discussionsupporting

confidence: 93%

“…In line with other studies (Darmishonnejad et al, 2020;Rocca et al, 2016;Tahamtan et al, 2019), our finding showed a high The telomere in spermatozoa is a repetitive sequence that protects the chromosome terminal from both endogenous and exogenous damage (Von Zglinicki, 2002). So it is more likely that shortened sperm telomere may contribute to sperm DNA damage in oligozoospermic individuals.…”

Section: Discussionsupporting

confidence: 91%

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Analysis of leukocyte and sperm telomere length in oligozoospermic men

et al. 2021

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Nowadays, researchers are stating that, in most cases, standard semen analyses on their own cannot discern the fertile from the infertile populations, and therefore, in addition to semen analysis, assessment of sperm functional tests, including sperm DNA damage, may assist clinicians in achieving this goal (Rocca et al., 2016).Telomeres are conserved repetitive DNA sequences found at the ends of chromosomes and play an important role in maintaining genomic integrity, chromosome pairing, and homologous chromosome synapsis. In addition, these sequences prevent chromosome end joining and facilitate meiosis II segregation. Telomeres are made up of noncoding hexameric tandem repeats (5′-TTAGGG-3′) of DNA and are linked to proteins at the chromosome's end (Li et al., 2017;Turner et al., 2019). The length of telomeres in somatic cells shortens with each cycle of cell division, and this shortening may be accelerated by diseases (cancers, obesity, diabetes and cardiovascular disease), smoking, social interaction, mental health conditions,

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 91%

Analysis of leukocyte and sperm telomere length in oligozoospermic men

et al. 2021

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“…Another layer of complexity in the aetiology of male infertility is due to the role of telomeres [25][26][27][28][29][30][31], with several studies investigating sperm telomere length (STL) and leukocyte telomere length (LTL) in male infertility [26,29,[31][32][33][34][35]. These studies have been conducted using different study designs, measuring techniques, exposure variables and outcomes, and have a limited sample size, ranging from 20 to 100 in Caucasian males.…”

Section: Introductionmentioning

confidence: 99%

Telomere Length and Male Fertility

Gentiluomo

Luddi

Cingolani

et al. 2021

IJMS

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Over the past decade, telomeres have attracted increasing attention due to the role they play in human fertility. However, conflicting results have been reported on the possible association between sperm telomere length (STL) and leukocyte telomere length (LTL) and the quality of the sperm parameters. The aim of this study was to run a comprehensive study to investigate the role of STL and LTL in male spermatogenesis and infertility. Moreover, the association between the sperm parameters and 11 candidate single nucleotide polymorphisms (SNPs), identified in the literature for their association with telomere length (TL), was investigated. We observed no associations between sperm parameters and STL nor LTL. For the individual SNPs, we observed five statistically significant associations with sperm parameters: considering a p < 0.05. Namely, ACYP2˗rs11125529 and decreased sperm motility (p = 0.03); PXK˗rs6772228 with a lower sperm count (p = 0.02); NAF1˗rs7675998 with increased probability of having abnormal acrosomes (p = 0.03) and abnormal flagellum (p = 0.04); ZNF208˗rs8105767 and reduction of sperms with normal heads (p = 0.009). This study suggests a moderate involvement of telomere length in male fertility; however, in our analyses four SNPs were weakly associated with sperm variables, suggesting the SNPs to be pleiotropic and involved in other regulatory mechanisms independent of telomere homeostasis, but involved in the spermatogenic process.

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“…In addition, our study did not find evidence of any correlation between TL in the male pronucleus and such parameters of sperm quality as concentration, progressive motility or normal morphology. While some studies registered correlations between TL in spermatozoa and total sperm count [ 54 , 55 ], sperm concentration [ 56 ], motility [ 57 , 58 ], sperm DNA fragmentation [ 57 , 58 ] and oligozoospermia [ 54 ], other studies did not show such associations [ 30 , 55 , 57 , 58 ]. There is a possible explanation for such discrepancies in the results.…”

Section: Discussionmentioning

confidence: 99%

Telomere Length in Metaphase Chromosomes of Human Triploid Zygotes

Pendina

Krapivin

Efimova

et al. 2021

IJMS

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The human lifespan is strongly influenced by telomere length (TL) which is defined in a zygote—when two highly specialised haploid cells form a new diploid organism. Although TL is a variable parameter, it fluctuates in a limited range. We aimed to establish the determining factors of TL in chromosomes of maternal and paternal origin in human triploid zygotes. Using Q-FISH, we examined TL in the metaphase chromosomes of 28 human triploid zygotes obtained from 22 couples. The chromosomes’ parental origin was identified immunocytochemically through weak DNA methylation and strong hydroxymethylation in the sperm-derived (paternal) chromosomes versus strong DNA methylation and weak hydroxymethylation in the oocyte-derived (maternal) ones. In 24 zygotes, one maternal and two paternal chromosome sets were identified, while the four remaining zygotes contained one paternal and two maternal sets. For each zygote, we compared mean relative TLs between parental chromosomes, identifying a significant difference in favour of the paternal chromosomes, which attests to a certain “imprinting” of these regions. Mean relative TLs in paternal or maternal chromosomes did not correlate with the respective parent’s age. Similarly, no correlation was observed between the mean relative TL and sperm quality parameters: concentration, progressive motility and normal morphology. Based on the comparison of TLs in chromosomes inherited from a single individual’s gametes with those in chromosomes inherited from different individuals’ gametes, we compared intraindividual (intercellular) and interindividual variability, obtaining significance in favour of the latter and thus validating the role of heredity in determining TL in zygotes. A comparison of the interchromatid TL differences across the chromosomes from sets of different parental origin with those from PHA-stimulated lymphocytes showed an absence of a significant difference between the maternal and paternal sets but a significant excess over the lymphocytes. Therefore, interchromatid TL differences are more pronounced in zygotes than in lymphocytes. To summarise, TL in human zygotes is determined both by heredity and parental origin; the input of other factors is possible within the individual’s reaction norm.

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Relationship between sperm telomere length and sperm quality in infertile men

Cited by 38 publications

References 43 publications

Analysis of leukocyte and sperm telomere length in oligozoospermic men

Analysis of leukocyte and sperm telomere length in oligozoospermic men

Telomere Length and Male Fertility

Telomere Length in Metaphase Chromosomes of Human Triploid Zygotes

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