Relationship between sex ratio and time of insemination according to both time of ovulation and maturational state of oocyte

Gutiérrez-Adán, A.; Pérez-Garnelo, S. S.; Granados, J; Garde, José Julián; Pérez-Guzmán, M. D.; Pintado, Beatriz; Fuente, J. de la

doi:10.1016/s0093-691x(99)91956-8

Cited by 18 publications

(27 citation statements)

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“…A similar, but much less prominent, imbalance in favor of males among in vitro-produced bovine blastocysts has been reported earlier by some [49][50][51][52][53] but not all publications [30]. The difference in sex ratio has been attributed to several factors such as gene expression [50,54]; detoxification of oxygen radicals [54]; developmental speed [49,52]; oocyte maturation and fertilization [51,52,55]; and laboratory conditions [55]. Moreover, Gutierrez-Á dan [52] found that higher glucose concentration in embryo culture medium is more detrimental to female embryos.…”

Section: Discussionsupporting

confidence: 82%

Post Hatching Development: a Novel System for Extended in Vitro Culture of Bovine Embryos

Brandão¹,

Maddox-Hyttel²,

Løvendahl³

et al. 2004

Biology of Reproduction

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Although acceptable rates of blastocyst formation are achieved with in vitro production of bovine embryos, several problems still compromise the subsequent development of the fetus and newborn, especially in embryos originating from somatic cell nuclear transfer. Routinely, the potential development of a bovine conceptus is predicted either on blastocyst quality or on various parameters related to the embryonic-fetal development in a foster mother. These methods are either imprecise or costly, highlighting the need for more reliable and practical methods to evaluate early embryonic development and differentiation. Thus, our aim was to improve the in vitro culture of embryos post hatching and to define a stable and repeatable system to monitor the development of bovine embryos. For that, in vitro-derived embryos were cultured in agarose gel tunnels in a modified culture medium (SOFaaci within 10% fetal bovine serum and 27.7 mM glucose). Daily monitoring of embryo length revealed that 56%-67% of the embryos in culture showed rapid growth and elongated until Day 13. Electron microscopy of elongated embryos at Day 14 confirmed successful localization of differentiated cells forming the trophoblast and hypoblast, with the definition of the Rauber layer. In conclusion, a stable culture system of post hatching embryos was first defined and can be used as a model for rapid growth, elongation, and initial differentiation of bovine post hatching embryos produced entirely in vitro.

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Section: Discussionsupporting

confidence: 82%

Post Hatching Development: a Novel System for Extended in Vitro Culture of Bovine Embryos

Brandão¹,

Maddox-Hyttel²,

Løvendahl³

et al. 2004

Biology of Reproduction

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“…The timing of insemination, in relation to the maturation state of an oocyte has also shown to influence the sex ratio of bovine pre‐attachment embryos (Dominko and First 1997; Gutierrez‐Adan et al 1999; Rorie 1999). Oocytes inseminated immediately after the first polar body (pb) extrusion or a few hours before ovulation in vivo , tend to develop into female embryos.…”

Section: Discussionmentioning

confidence: 99%

Sperm Pre‐Incubation Prior to Insemination Affects the Sex Ratio of Bovine Embryos Produced in vitro

Lechniak¹,

Strabel²,

Bousquet

et al. 2003

Reprod Domestic Animals

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The objective of the present study was to determine whether sperm incubation prior to oocyte insemination in vitro affects the sex ratio of resulting blastocyst. Cumulus-oocyte-complexes (COCs) collected from slaughterhouse ovaries were matured in vitro and inseminated with frozen-thawed semen of three proven artificial insemination (AI) bulls pre-incubated in vitro in Sperm-Talp for 6 and 24 h. On day-9 blastocysts were collected and processed for sex determination. More than 80% of blastocyst were successfully sexed. There were no significant differences in cleavage and blastocyst rates using sperm pre-incubated for 6 h as compared with the 0-h pre-incubation control group. The cleavage and blastocyst rates were significantly lower in the 24-h pre-incubation group. The male to female ratio, when compared with the theoretical 1 : 1, differed significantly in favour of females among hatched (viable) blastocysts derived from sperm pre-incubated for 24 h prior to insemination as well as among all blastocytsts in the 6-h group. Moreover, when the sperm treatment was considered, the sex ratio was affected only among hatched blastocysts in 24-h pre-incubation group. It was concluded that prolonged sperm pre-incubation influences the rate of development and the sex ratio among hatched blastocysts.

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“…Several factors have been reported to influence sex ratio in cattle including: (i) timing of insemination in vivo (Gutierrez‐Adan et al. 1999; Rorie 1999; Roelofs et al.…”

Section: Post‐fertilization Culture Environment and Sex Ratiomentioning

confidence: 99%

Consequences of In Vitro Culture Conditions on Embryo Development and Quality

Rizos

Clemente

Bermejo-Álvarez

et al. 2008

Reprod Domestic Animals

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115

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Despite major efforts directed at improving the yield of blastocysts from immature oocytes in vitro, the quality of such blastocysts continually lags behind that of blastocysts produced in vivo. These differences are manifested at the level of morphology, metabolism, gene expression and cryotolerance, and may have a knock-on effect further along the developmental axis. Evidence suggesting that in vitro culture conditions, while capable of producing blastocysts in relatively high numbers, are far from optimal with deficiencies being manifested in terms of abnormally large offspring. It is clear nowadays that modification of the post-fertilization culture environment in vitro can improve blastocyst quality to some extent.

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Relationship between sex ratio and time of insemination according to both time of ovulation and maturational state of oocyte

Cited by 18 publications

References 0 publications

Post Hatching Development: a Novel System for Extended in Vitro Culture of Bovine Embryos

Post Hatching Development: a Novel System for Extended in Vitro Culture of Bovine Embryos

Sperm Pre‐Incubation Prior to Insemination Affects the Sex Ratio of Bovine Embryos Produced in vitro

Consequences of In Vitro Culture Conditions on Embryo Development and Quality

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